[미국특허]
Methods and compositions for polypeptide engineering
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/68
C12P-019/34
C07K-014/00
C07H-021/04
출원번호
US-0559671
(2000-04-27)
발명자
/ 주소
Patten, Phillip A.
Stemmer, Willem P. C.
출원인 / 주소
Maxygen, Inc.
대리인 / 주소
Kruse, Norman J.Sappenfield, Christopher C.Quine, Jonathan A.
인용정보
피인용 횟수 :
32인용 특허 :
124
초록
Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
대표청구항▼
Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed. or replacing at least a portion of hydrogen atoms of a polyvinylphenolic polymeric m
Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed. or replacing at least a portion of hydrogen atoms of a polyvinylphenolic polymeric material by a substituent containing a propyl group, or replacing at least a portion of hydrogen atoms of a siloxane polymeric material by a substituent containing a propyl group and/or an aminopropyl group, or replacing at least a portion of hydrogen atoms of an acrylic polymeric material by a substituent containing a phenyl group and/or a naphthalene group, or replacing at least a portion of hydrogen atoms of a polyvinylphenolic polymeric material by a substituent containing a phenyl group, a naphthalene group and/or a methylphenyl group, or replacing at least a portion of hydrogen atoms of a siloxane polymeric material by a substituent containing a phenyl group and/or a naphthalene group, said hydrogen atoms being replaced to provide a line absorption coefficient in an X-ray wavelength range of the polymeric material constituting said resist material of 3.80μ-1or less; and exposing said resist layer selectively to X-rays, vacuum ultraviolet rays, extreme ultraviolet rays or soft X-rays to pattern the resist layer to a pre-set shape. 8. The light exposure method according to claim 7, wherein the wavelength of said ultraviolet rays is 7 to 16 nm. he photographic material having a dry layer thickness of not more than 20 μm. ein the fatty acid silver salt is produced by a producing method, said producing method comprising: adding (1) a solution of silver ions comprising water or a mixed solution of an organic solvent and water containing therein silver ions, and (2) a solution of a fatty acid alkali metal salt which is a solution or a suspension comprising water, an organic solvent, or a mixed solution of water and an organic solvent, containing therein an alkali metal salt of a fatty acid to a closed mixing means to react the solution (1) and the solution (2), wherein from 50 to 99.5 mol % of the entire fatty acid alkali metal salt solution is added to the closed mixing means under such a condition that the concentration of the fatty acid alkali metal salt is higher than the silver ion concentration, and from 0.5 to 30 mol % of the entire fatty acid alkali metal salt solution is added to the closed mixing means or downstream of the closed mixing means after the silver ion solution has been added to the closed mixing means; said photothermographic image-recording material further contains a photosensitive silver halide on a support; and the ratio of the aqueous latex solid content weight to the fatty acid silver weight in the layer containing the fatty acid silver salt is from 1.0 to 2.5. 12. The photothermographic image-recording material as claimed in claim 11, wherein from 0.5 to 30 mol % of the entire fatty acid alkali metal salt solution is added to a formation tank equipped downstream from the closed mixing means after the silver ion solution has been added to the closed mixing means. 13. The photothermographic image-recording material of claim 11, wherein said fatty acid silver salt is a scaly fatty acid silver salt. 14. The photothermographic image-recording material of claim 11, wherein said salt of the alkali metal of the fatty acid is selected from the group consisting of a Na salt, a K salt and a Li salt. 15. The photothermographic image-recording material of claim 11, wherein said fatty acid is a long chain aliphatic carboxylic acid having 10 to 30 carbon atoms. 16. The photothermographic image-recording material of claim 11, wherein said fatty acid is selected from the group consisting of cerotic acid, lignoceric acid, behenic acid, erucic acid, arachidic acid, stearic acid, oleic acid, lauric acid, caproic acid, myristic acid, palmitic acid, maleic acid, fumaric acid, tartaric acid, linoleic acid, butyric acid, camphoric acid and mixtures of any of said acids. 17. The photothermographic image-recording material of claim 11, wherein 70 to 95 mol % of said entire fatty acid alkali metal salt solution is added to the closed mixing means. 18. The photothermographic image-recording material of claim 11, wherein said solution (1) or solution (2) further comprises an auxiliary dispersant. 19. The photothermographic image-recording material of claim 12, wherein 3 to 20 mol % of the entire fatty acid alkali metal salt solution is added to the formation tank equipped downstream from the closed mixing means after the silver ion solution has been added to the closed mixing means. 20. The photothermographic image-recording material of claim 11, wherein said photothermographic image-recording material comprises 0.1 to 5 g/m2of said fatty acid silver salt.
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