IPC분류정보
| 국가/구분 |
United States(US) Patent
등록
|
| 국제특허분류(IPC7판) |
|
| 출원번호 |
US-0831425
(2001-05-07)
|
| 국제출원번호 |
PCT/US99/23386
(1999-10-06)
|
| 국제공개번호 |
WO00/21053
(2000-04-13)
|
|
발명자
/ 주소 |
- Marman, Douglas H.
- Liu, Kai Bang
|
| 출원인 / 주소 |
|
| 대리인 / 주소 |
|
| 인용정보 |
피인용 횟수 :
265 인용 특허 :
20 |
초록
▼
A wireless alarm system (10) employs two-way transceivers (32, 60) in a network of smoke detectors (16), a base station (12), and other sensors. A keypad (14) is not needed because the system is reset by pressing a Test/Silence button (66) built into every detector or sensor. A siren is also elimina
A wireless alarm system (10) employs two-way transceivers (32, 60) in a network of smoke detectors (16), a base station (12), and other sensors. A keypad (14) is not needed because the system is reset by pressing a Test/Silence button (66) built into every detector or sensor. A siren is also eliminated because a sounder (64) in every detector sounds an alarm when any sensor is triggered. This is possible because every detector includes a transceiver that can receive alarm messages from any other detector. AC power wiring is also eliminated because the base station and sensors are battery powered. Only a telephone connection (48) is needed if the system is to be monitored. In apartments or dormitory installations, smoke detectors in one apartment relay alarm messages to the next apartment, and onto the next, and so on, to a centralized base station for the entire facility. The centralized base station can be located in an apartment manager's office for immediate notification of an alarm, improper smoke detector operation, low or missing battery indications, and dirty smoke detector indications. The two-way wireless alarm system can save many lives in apartments, where smoke detectors batteries are often depleted or removed.
대표청구항
▼
A wireless alarm system (10) employs two-way transceivers (32, 60) in a network of smoke detectors (16), a base station (12), and other sensors. A keypad (14) is not needed because the system is reset by pressing a Test/Silence button (66) built into every detector or sensor. A siren is also elimina
A wireless alarm system (10) employs two-way transceivers (32, 60) in a network of smoke detectors (16), a base station (12), and other sensors. A keypad (14) is not needed because the system is reset by pressing a Test/Silence button (66) built into every detector or sensor. A siren is also eliminated because a sounder (64) in every detector sounds an alarm when any sensor is triggered. This is possible because every detector includes a transceiver that can receive alarm messages from any other detector. AC power wiring is also eliminated because the base station and sensors are battery powered. Only a telephone connection (48) is needed if the system is to be monitored. In apartments or dormitory installations, smoke detectors in one apartment relay alarm messages to the next apartment, and onto the next, and so on, to a centralized base station for the entire facility. The centralized base station can be located in an apartment manager's office for immediate notification of an alarm, improper smoke detector operation, low or missing battery indications, and dirty smoke detector indications. The two-way wireless alarm system can save many lives in apartments, where smoke detectors batteries are often depleted or removed. d host cell population having a second predetermined level of reporter gene expression which is distinct from said first predetermined level; and (g) recovering cell-type specific cis-regulatory elements from said members having said distinct second predetermined level of reporter gene expression. 3. The method of claim 1 or 2, further comprising reintroducing the cell-type specific cis-regulatory elements recovered in step (g) into the first population of host cells, and repeating steps (c) through (g). 4. The method of claim 3, wherein the cell-type specific cis-regulatory elements recovered in step (g) are manipulated in vitro prior to the reintroducing step. 5. The method of claim 4, wherein the manipulation in vitro is alteration of the cell-type specific cis-regulatory elements by a technique selected from the group consisting of mutation in vitro and recombination in vitro. 6. The method of claim 1 or 2, further comprising reintroducing the pooled sub-library of oligonuclootides recovered in step (d) into the first population of host cells, and repeating step (c). 7. The method of claim 1 or 2, further comprising reintroducing the cell type specific cis-regulatory elements recovered in step (g) into the second population of host cells, and repeating step (f). 8. The method of claim 1 or 2, wherein the pooled sub-library of oligonucleotides recovered in step (d) and the cell-type specific cis-regulatory elements recovered in step (g) include expression vector sequences. 9. The method of claim 1 or 2, wherein the expression vector further comprises a second sequence encoding a second reporter molecule. 10. The method of claim 1 or 2, wherein the steps of selecting comprise the use of a fluorescence activated cell sorter. 11. The method of claim 1, wherein said library of synthetic oligonucleotides comprises all possible sequences of a pre-selected length of up to about 10. 12. The method of claim 1, wherein said library of synthetic oligonucleotides comprises a subset of all possible sequences of a pre-selected length. ut 55 bases including about 22 bases consisting essentially of LTR-specific bases contained in SEQ ID NO:2 and at least one oligomer that hybridizes specifically to a pol sequence. 6. The method of claim 1, wherein the amplifying step uses at least two amplification oligomers that bind specifically to LTR or pol sequences or sequences complementary to LTR or pol sequences. 7. The method of claim 6, wherein the amplifying step uses amplification oligomers for amplifying LTR sequences consisting essentially of SEQ ID NO:7 and SEQ ID NO:9. 8. The method of claim 6, wherein the amplifying step uses at least two amplification oligomers for amplifying pol sequences consisting essentially of sequences of: SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 or SEQ ID NO:14. 9. The method of claim 1, wherein the amplifying step comprises a transcription-associated amplification method that uses: at least one promoter-primer comprising a promoter sequence that is recognized by an RNA polymerase when the promoter sequence is double stranded, wherein the promoter sequence is covalently attached to the 5' end of a LTR-specific sequence consisting essentially of SEQ ID NO:7, or a pol-specific sequence consisting essentially of SEQ ID NO:12 or SEQ ID NO:14; and at least one primer selected from a LTR-specific sequence consisting essentially of SEQ ID NO:9, or a pol-specific sequence consisting essentially of SEQ ID NO:10 or SEQ ID NO:11, provided that the LTR-specific promoter-primer is combined with the LTR-specific primer for amplifying a LTR target region, and/or at least one pol-specific promoter-primer is combined with at least one pol-specifics primer for amplifying a pol target region. 10. The method of claim 9, wherein the amplifying step uses any of the following combinations of promoter-primers and primers: promoter-primers consisting essentially of SEQ ID NO:12 and SEQ ID NO:14, with primers consisting essentially of SEQ ID NO:10 and SEQ ID NO:11; promoter-primers consisting essentially of SEQ ID NO:7, SEQ ID NO:12 and SEQ ID NO:14, with primers consisting essentially of SEQ ID NO:9, SEQ ID NO:10 and SEQ ID NO:11; a promoter-primer consisting essentially of SEQ ID NO:12, and a primer consisting essentially of SEQ ID NO:10; or a promoter-primer consisting essentially of SEQ ID NO:7, and a primer consisting essentially of SEQ ID NO:9. 11. The method of claim 1, wherein the detecting step uses at least one labeled detection probe having a base sequence selected from: a LTR-specific sequence consisting essentially of SEQ ID NO:16, a pol-specific sequence consisting essentially of SEQ ID NO:17, or a pol-specific sequence consisting essentially of SEQ ID NO:18. 12. The method of claim 1, wherein the detecting step uses a combination of at least two labeled detection probes, wherein the probe base sequences are about 20 bases consisting essentially of SEQ ID NO:16, about 22 to about 30 bases consisting essentially of SEQ ID NO:17, or about 17 to about 20 bases consisting essentially of SEQ ID NO:18. 13. The method of claim 12, wherein the labeled detection probe consisting essentially of SEQ ID NO:16 has an inosine at position 7. 14. The method of claim 1, wherein the detecting step uses a labeled detection probe of about 20 bases consisting essentially of SEQ ID NO:16. 15. The method of claim 1, wherein the detecting step uses at least one labeled detection probe of about 22 to about 30 bases consisting essentially of SEQ ID NO:17, or about 17 to about 20 bases consisting essentially of SEQ ID NO:18. 16. The method of claim 1, wherein the detecting step uses at least one labeled detection probe that includes at least one 2'-methoxy backbone linkage. 17. The method of claim 1, wherein: the contacting step uses capture oligomers made up of LTR-specific bases consisting essentially of about 22 LTR-specific bases contained in SEQ ID NO:2 and a tail sequence of about 5 to 50 bases that are not LTR-specific and provide a means of capturing HIV-1 nucleic acid hybridized to the LTR-specific sequence from the other components in the biological sample; the amplifying step uses promoter-primers consisting essentially of the sequences of SEQ ID NO:7, SEQ ID NO:12 and SEQ ID NO:14 and primers consisting essentially of the sequences of SEQ ID NO:9, SEQ ID NO:10 and SEQ ID NO:11; and the detecting step uses labeled detection probes wherein probe base sequences are about 20 bases consisting essentially of SEQ ID NO:16, about 22 to about 30 bases consisting essentially of SEQ ID NO:17 and about 17 to about 20 bases consisting essentially of SEQ ID NO:18. 18. The method of claim 1, wherein: the contacting step uses at least two capture oligomers that hybridize to different sequences in the target region; the amplifying step uses at least two different promoter-primers that hybridize to a first set of sequences within the target region and at least two different primers that hybridize to a second set of sequences within the target region; and the detecting step uses at least two labeled probes that bind specifically to different sequences located between the first set and second set of sequences within the target region. 19. The method of claim 18, wherein: the contacting step uses capture oligomers having sequences that hybridize specifically to pol sequences; the amplifying step uses promoter-primers consisting essentially of sequences of SEQ ID NO:12 and SEQ ID NO:14 and primers consisting essentially of the sequences of SEQ ID NO:10 and SEQ ID NO:11; and the detecting step uses labeled probes of about 22 to about 30 bases consisting essentially of SEQ ID NO:17 and about 17 to about 20 bases consisting essentially of SEQ ID NO:18. 20. The method of claim 18, wherein the amplifying step uses at least two promoter-primers that hybridize to a first set of overlapping sequences within the target region, at least two primers that hybridize to a second set of overlapping sequences within the target region, or at least two promoter-primers that hybridize to a first set of overlapping sequences within the target region and at least two primers that hybridize to a second set of overlapping sequences within the target region. 21. A kit comprising a combination of oligomers, wherein the oligomers contained in the kit have sequences consisting essentially of SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:17 and SEQ ID NO:18, and wherein the oligomers consisting essentially of the sequences of SEQ ID NO:17 and SEQ ID NO:18 are labeled with a detectable label. 22. The kit of claim 21, also containing oligomers with base sequences consisting essentially of the sequences of SEQ ID NO:7, SEQ ID NO:9 and SEQ ID NO:16, wherein the oligomer consisting essentially of SEQ ID NO:16 is labeled with a detectable label. 23. The method of claim 21, wherein the oligomer consisting essentially of SEQ ID NO:16 has an inosine at position 7. 24. A composition comprising a combination of at least two oligomers selected from the group consisting of: SEQ ID NO:7, optionally with a promoter sequence covalently attached to the 5' end of SEQ ID NO:7; and SEQ ID NO;9. 25. The composition of claim 24, wherein the promoter sequence is a T7 RNA polymerase promoter sequence. 26. The composition of claim 24, wherein SEQ ID NO:7 with the promoter sequence covalently attached to the 5' end is SEQ ID NO:8. 27. The composition of claim 24, wherein the composition further comprises an oligomer of SEQ ID NO:16 containing an inosine base. 28. The composition of claim 27, wherein an oligomer base sequence is linked by a backbone that includes at least one 2'-methoxy RNA group, at least one 2' fluoro-substituted RNA group, at least one peptide nucleic acid linkage, at least one phosphorohioate linkage, at least one methylphosphonate linkage or any combination thereof. 29. The composition of claim 27, wherein the oligomer of SEQ ID NO:16 containing an inosine base comprises at
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