초록 ▼

A system and method for real time monitoring and control of energy consumption at a number of facilities to allow aggregate control over the power consumption. A central location receives information over a communications network, such as a wireless network, from nodes placed at facilities. The node

A system and method for real time monitoring and control of energy consumption at a number of facilities to allow aggregate control over the power consumption. A central location receives information over a communications network, such as a wireless network, from nodes placed at facilities. The nodes communicate with devices within the facility that monitor power consumption, and control electrical devices within the facility. The electrical devices may be activated or deactivated remotely by the central location. This provides the ability to load balance a power consumption grid and thereby proactively conserve power consumption as well as avoid expensive spikes in power consumption. The present invention also includes a wireless network for communicating with the number of facilities, and which allows other information to be collected and processed.

대표청구항 ▼

A system and method for real time monitoring and control of energy consumption at a number of facilities to allow aggregate control over the power consumption. A central location receives information over a communications network, such as a wireless network, from nodes placed at facilities. The node

A system and method for real time monitoring and control of energy consumption at a number of facilities to allow aggregate control over the power consumption. A central location receives information over a communications network, such as a wireless network, from nodes placed at facilities. The nodes communicate with devices within the facility that monitor power consumption, and control electrical devices within the facility. The electrical devices may be activated or deactivated remotely by the central location. This provides the ability to load balance a power consumption grid and thereby proactively conserve power consumption as well as avoid expensive spikes in power consumption. The present invention also includes a wireless network for communicating with the number of facilities, and which allows other information to be collected and processed. r at the cDNA's second terminus; (f) generating a cRNA preparation of antisense cRNA transcripts by incubation of the linearized fragments with a bacteriophage-specific RNA polymerase capable of initiating transcription from the bacteriophage-specific promoter; (g) dividing the cRNA preparation into subpools and transcribing first-strand cDNA from each subpool, using a reverse transcriptase and a 5'-RT primer, the 5'-RT primers defined as having a 3'-terminus consisting of -Nx,wherein "N" is one of the four deoxyribonucleotides A, C, G, or T, and "x" is an integer from 1 to 5, the primer being 15 to 30 nucleotides in length and complementary to the 5' flanking vector sequence with the primer's complementarity extending across into the insert-specific nucleotides of the cRNA in a number of nucleotides equal to "x", wherein a different one of said primers is used in different subpools and wherein there are 4 subpools if "x"=1, 16 subpools if "x"=2, 64 subpools if "x"=3,256 subpools if "x"=4, and 1,024 subpools if "x"=5; (h) Using the product of first-strand cDNA produced by transcribing each of the subpools as a template for a polymerase chain reaction with a 3'-PCR primer of 15 to 30 nucleotides in length that is complementary to 3' flanking vector sequences between said first restriction endonuclease site and the site defining transcription initiation by the bacteriophage-specific promoter and a 5'-PCR primer having a 3'-terminus consisting of -Nx-Ny,where "N" and "x" are as in step (g), -Nxis the same sequence as in the 5'-RT primer from which first-strand cDNA was made for that subpool, and "y" is a whole integer such that x+y equals an integer selected from the group consisting of 3, 4, 5 and 6, the 5'-PCR primer being 15 to 30 nucleotides in length and complementary to the 5' flanking vector sequence with the primer's complementarity extending across into the insert-specific nucleotides of the cRNA in a number of nucleotides equal to "x+y", to produce polymerase chain reaction amplified fragments; (i) resolving the polymerase chain reaction amplified fragments to generate a display of sequence-specific products representing the 3'-ends of different mRNAs present in the mRNA population; (j) determining the sequence of a 5' component of a sequence identifier of each sequence-specific product, the sequence of a 3' component of a sequence identifier of each sequence-specific product, the length of each sequence-specific product, and the intensity of labeling of each sequence-specific product; (k) storing data comprising the sequence of a 5' component of a sequence identifier of each sequence-specific product, the sequence of a 3' component of a sequence identifier of each sequence-specific product, the length of each sequence-specific product; and the intensity of labeling of each sequence-specific product, on a computer readable medium; and (l) comparing the data stored on the computer readable medium to indicate sequence relationship matches between sequence-specific products and compare levels of expression. 2. The method of claim 1 wherein the step of comparing comprises comparing the data stored on the computer readable medium to data in a database. 3. A method of comparing mRNA sequence data from a first RNA sample to mRNA sequence data from a second RNA sample using a database comprising the steps of: (a) isolating an mRNA population from a first RNA sample; (b) by performing steps (a)-(k) of claim 1 to provide stored data comprising the sequence of a 5' component of a sequence identifier of each sequence-specific product, the sequence of a 3' component of a sequence identifier of each sequence-specific product, the length of each sequence-specific product; and the intensity of labeling of each sequence-specific product on a computer readable medium representing the 3'-ends of mRNAs present in the first sample; (c) isolating an mRNA population from a second RNA s