Self-contained device integrating nucleic acid extraction, amplification and detection
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12P-019/34
C12M-001/34
C07H-021/04
G01N-030/96
G01N-030/02
출원번호
US-0705043
(2000-11-02)
발명자
/ 주소
Kozwich, Diane L.
Gerdes, John C.
출원인 / 주소
Xtrana, Inc.
대리인 / 주소
Petersen, Steven C.O'Rourke, Sarah S.Hogan & Hartson LLP
인용정보
피인용 횟수 :
32인용 특허 :
14
초록▼
Self-contained devices are described that integrate nucleic acid extraction, specific target amplification and detection into a single device. This integration permits rapid and accurate nucleic acid sequence detection. The invention may be used, for example, in the screening for nucleic acid sequen
Self-contained devices are described that integrate nucleic acid extraction, specific target amplification and detection into a single device. This integration permits rapid and accurate nucleic acid sequence detection. The invention may be used, for example, in the screening for nucleic acid sequences which may be indicative of genetic defects or contagious diseases, as well as for monitoring efficacy in the treatment of contagious diseases.
대표청구항▼
Self-contained devices are described that integrate nucleic acid extraction, specific target amplification and detection into a single device. This integration permits rapid and accurate nucleic acid sequence detection. The invention may be used, for example, in the screening for nucleic acid sequen
Self-contained devices are described that integrate nucleic acid extraction, specific target amplification and detection into a single device. This integration permits rapid and accurate nucleic acid sequence detection. The invention may be used, for example, in the screening for nucleic acid sequences which may be indicative of genetic defects or contagious diseases, as well as for monitoring efficacy in the treatment of contagious diseases. 3-160. Carbonell, L. et al., "Synthesis of Gene Coding for an Insect-Specific Scorpion Neurotoxin and Attempts to Express Its Using Baculovirus Vectors", Gene, 1988, 73, 409-418. Cerrone, M. et al., "Cloning and Sequence of the Gene for Heat Shock Protein 60 from Chlamydia trachomatis and Immunological Reactivity of the Protein", Infect. Immun., 1991, 59(1), 79-90. Chassy, B. and Flickinger, "Transformation of Lactobacillus casei by Electroporation", FEMS Microbiology Letters, 1987, 44, 173-177. Cohen, J. et al., "Functional Expression in Yeast of the Escherichia coli Plasmis Gene Coding for Chloramphenicol Acetyltransferase", PNAS USA, 1980, 77(2), 1078-1082. Cohen, S. et al., "Nonchromosomal Antibiotic Resistance in Bacterial: Genetic Transformation of Escherichia coli by R-Factor DNA", PNAS USA, 1972, 69(8), 2110-2114. Cregg, J. et al., "Pichia pastoris as a Host System for Transformations", Mol. Cell. Biol., 1985, 5(12), 3376-3385. Das, S. et al., "Transformation of Kluyveromyces fragilis", J. Bacteriol., 1984, 158(3), 1165-1167. Davidow, L. et al., "Integrative Transformation of the Yeast Yarrowia lipolytica", Curr. Genet., 1985, 10, 39-48. Davies, J. and Smith, "Plasmid-Determined Resistance to Antimicrobial Agents", Ann. Rev. Microbiol., 1978, 32, 469-518. De Boer, H. et al., "The TAC Promoter: A Functional Hybrid Derived from the trp and lac Promoters", PNAS USA, 1983, 80, 21-25. De Louvencourt, L. et al., "Transformation of Kluyveromyces lactis by Killer Plasmid DNA", J. Bacteriol., 1983, 154(2), 737-742. Dijkema, R. et al., "Cloning and Expresssion of the Chromosomal Immune Interferon Gene of the Rat", EMBO J., 1985, 4(3), 761-767. Dower, W. et al., "High Efficiency Transformation of E. Coli by High Voltage Electroporation", Nucl. Acids Res., 1988, 16(13), 6127-6145. Fiedler, S. and Wirth, "Transformation of Bacteria with Plasmid DNA by Electroporation", Analytical Biochem., 1988, 170, 38-44. Fraser, M.J., "Expression of Eucaryotic Genes in Insect Cell Cultures", In Vitro Cellular & Developmental Biology, 1989, 25(3), Part I, 225-235. Friesen, P.D. and Miller, "The Regulation of Baculovirus Gene Expression", Current Topics in Microbiology, 1986, 131, 31-49. Gaillardin, C. et al., "Integrative Transformation of the Yeast Yarrowia lipolytica", Curr. Genet., 1985, 10, 49-58. Ghrayeb, J. et al., "Secretion Cloning Vectors in Escherichia coli", EMBO J., 1984, 3(10), 2437-2442. Gleeson, M.A. et al., "Transformation of the Methylotrophic Yeast Hansenula polymorpha", J. Gen. Microbiol., 1986, 132, 3459-3465. Gluzman, Y., "SV40-Transformed Simian Cells Support the Replication of Early SV40 Mutants", Cell, 1981, 23, 175-182. Goeddel, D. et al., "Synthesis of Human Fibroblast Interferon by E. coli", Nucl. Acids Res., 1980, 8(18), 4057-4074. Gorman, C. et al., "The Rous Sarcoma Virus Long Terminal Repeat is a Strong Promoter When Introduced Into a Variety of Eukaryotic Cells By DNA-Mediated Transfection", PNAS USA, 1982, 79, 6777-6781. Green, M.R., "Pre-mRNA Splicing", Ann. Rev. Genet., 1986, 20, 671-708. Harlander, S., "Transformation of Streptococcus lactis by Electroporation", in Streptococcal Genetics, Ferretti and Curtiss III (eds.), Am. Soc. For Microbiology, Washington, D.C., 1987. Hatt, C. et al., "Analysis of the Entire Nucleotide Sequence of the Cryptic Plasmid of Chlamydia trachomatis Serovar L1. Evidence for Involvement in DNA Replication", Nucl. Acids Res., 1988, 16(9), 4053-4067. Henikoff, S. et al., "Isolation of a Gene from Drosophila By Complementation in Yeast", Nature, 1981, 289, 33-37. Hinnen, A. et al., "Transformation of Yeast", PNAS USA, 1978, 75(4), 1929-1933. Hollenberg, C., "The Expression of Bacterial Antibiotic Resistance Genes in the Yeast Saccharomyces cerevisiae", in Plasmids of Medical, Environmental and Commercial Importance, Timmis and Puhler (eds.), Elsevier/North-Holland Biomedical Press, 1979, pp. 481-492. Hollenberg, C.P., "Cloning with 20-μm DNA Vectors and the Expression of Foreign Genes in Saccharomyces cerevisiae", Curr. Topics Microbiol. Immunol., 1981, 96, 119-144. Ito, H. et al., "Transformation of Intact Yeast Cells Treated with Alkali Cations", J. Bacteriol. 1983, 153(1), 163-168. Jia, S. et al., "Carbohydrate-Binding Protein 35: Molecular Cloning and Expression of a Recombinant Polypeptide with Lectin Activity in Escherichia coli", Gene, 1987, 60, 197-204. Ju, Y.S. et al., "Effect of Naloxon on Counter Insulin Hormone Secretion in Insulin-Induced Hypoglycemia", The Korean J. Internal Med., 1987, 2(2), 190-195. Kaufman, R. et al., "The Phosphorylation State of Eucaryotic Initiation Factor 2 Alters Translational Efficiency of Specific mRNAs", Mol. Cell. Biol., 1989, 9(3), 946-958. Krainer, A. and Maniatis, "RNA Splicing", in Transcription and Splicing, Hames and Glover (eds.), IRL Press, pp. 131-207. Kunze, G. et al., "Transformation of the Industrially Important Yeasts Candida maltosa and Pichia guilliermondii", J. Basic Microbiol., 1985, 25(2), 141-144. Kurtz, M. et al., "Integrative Transformation of Candida albicans, Using a Cloned Candida ADE2 Gene", Mol. and Cell. Biol., 1986, 6(1), 142-149. Kushner, S., "An Improved Method for Transformation of Escherichia coli With ColE1 Derived Plasmids", in Genetic Engineering, Proceedings of the Int. Symposium on Genetic Engineering, Milan, Italy, Mar. 29-31, 1978, published by Elsevier/North-Holland Press, Amsterdam, 17-23. Lebacq-Verheyden, A.-M. et al., "Posttranslational Processing of Endogenous and of Baculovirus-Expressed Human Gastrin-Releasing Peptide Precursor", Mol. Cell. Biol., 1988, 8(8), 3129-3135. Luckow, V. and Summers, "High Level Expression of Nonfused Foriegn Genes with Autographa californica Nuclear Polyhedrosis Virus Expression Vectors", Virology, 1989, 170, 31-39. Maeda, S. et al., "Production of Human α-Interferon in Silkworm Using a Baculovirus Vector", Nature, 1985, 315, 592-594. Makoff, A. et al., "Translational Pusions with Fragments of the trpE Gene Improve the Expression of a Poorly Expressed Heterologous Gene in Escherichia coli", J. Gen. Microbiol., 1989, 135, 11-24. Mandel, M. and Higa, "Calcium-Dependent Bacteriophage DNA Infection", J. Mol. Biol., 1970, 53, 159-162. Maniatis, T. et al., "Regulation of Inducible and Tissue-Specific Gene Expression", Science, 1987, 236, 1237-1245. Martin, B. et al., "Glycosylation and Processing of High Levels of Active Human Glucocerebrosidase in Invertebrate Cells Using a Baculovirus Expression Vector", DNA, 1988, 7(2), 99-106. Masson, L. et al., "Transformation of Bacillus thuringiensis Vegetative Cells by Electroporation", FEMS Microbiology Letters, 1989, 60, 273-278. Mercereau-Puijalon, O. et al., "Synthesis of a Chicken Ovalbumin-Like Protein in the Yeast Saccharomyces cerevisiae", Gene, 1980, 11, 163-167. Miller, H. et al., "Cloning and Expression of a Yeast Ubiquitin-Protein Cleaving Activity in Escherichia coli", Bio/Technology, 1989, 7, 698-704. Miller, L., "Insect Baculoviruses: Powerful Gene Expression Vectors", BioEssays, 1989, 11(4), 91-95. Miller, J. et al., "High-Voltage Electroporation of Bacteria: Genetic Transformation of Campylobacter jejuni With Plasmid DNA", PNAS USA, 1988, 85, 856-860. Miller,L., "Baculoviruses as Gene Expression Vectors", Ann. Rev. Microbiol., 1988, 42, 177-199. Miyajima, A. et al., "Use of the Silkworm, Bombyx mori, and an Insect Baculovirus Vector for High-Level Expression and Secretion of Biologically Active Mouse Interleukin-3", Gene, 1987, 58, 273-281. Miyanohara, A. et al., "Expression of Hepatitis B Surface Antigen Gene in Yeast", PNAS USA, 1983, 80, 1-5. Nagai, K. and Thogersen, "Generation of β-Globin by Sequence-Specific Proteolysis of a Hybrid Protein produced in Escherichia coli", Nature, 1984, 309, 810-812. Nevins, J., "The Pathway of Eukaryotic mRNA Formation", Ann. Rev. Biochem., 1983, 52, 441-466. Oka, T. et al., "Synthesis and Secretion of Human Epidermal Growth Factor by Escherichia coli", PNAS USA, 1985, 82, 7212-7216. Orr-Weaver, T. et al., "Genetic Applications of Yeast Transformation with Linear and Gapped Plasmids", Methods in Enzymology, 1983, 101, 228-245. Padgett, R. et al., "Splicing of Messenger RNA Precursors", Ann. Rev. Biochem., 1986, 55, 1119-1150. Palva, I. et al., "Secretion of Escherichia coli β-Lactamase from Bacillus subtilis by the Aid of α-Amylase Signal Sequence", PNAS USA, 1982, 79, 5582-5586. Panthier, J.-J. et al., "Cloned β-Galactosidase Gene of Escherichia coli is Expressed in the Yeast Saccharomyces cerevisiae", Current Genetics, 1980, 2, 109-113. Perry, D. and Kuramitsu, "Genetic Transformation of Streptococcus mutans", Infection and Immunity, 1981, 22(3), 1295-1297. Powell, I. et al., "A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation". Appl. Environ. Microbiol., 1988, 54(3), 655-660. Proudfoot, N.J. and Whitelaw, "Termination and 3' End Processing of Eukarytic RNA", in Transcription and Splicing, Hames, B.D. and Glover (Eds.), IRL Press, Oxford, 1988. Proudfoot, N.J., "How RNA Polymerase II Terminates Transcription in High Eukaryotes", TIBS, 1989, 14, 105-110. Raibaud, O. and Schwartz, "Positive Control of Transcription Initiation in Bacteria", Ann. Rev. Genet., 1984, 18, 173-206. Rine, J. et al., "Targeted Selection of Recombinant Clones Through Gene Dosage Effects", PNAS USA, 1983, 80, 6750-6754. Roggenkamp, R. et al., "Transformation of the Methylotrophic Yeast Hansenula polymorpha by Automonous Replication and Integration Vectors", Mol. Gen. Genet., 1986, 202, 302-308. Saiki, R. et al., "Analysis of Enzymatically Amplified β-Globin and HLA-DQα DNA with Allele-Specific Oligonucleotide Probes", Nature, 1986, 324, 163-166. Sambrook, "Molecular Cloning A Laboratory Manual", Chapter 17, Cold Spring Harbor Press, 1989. Sassone-Corsi and Borrelli, "Transcriptional Regulation by trans-Acting Factors", TIBS, 1986, 215-219. Scharf, S. et al., "Direct Cloning and Sequence Analyis of Enzymatically Amplified Genomic Sequences", Science, 1986, 1076-1078. Shimatake, H. and Rosenberg, "Purified λ Regulatory Protein cII Positively Activates Promoters for Lysogenic Development", Nature, 1981, 292, 128-132. Shimizu, Y. et al., "Transfer of Cloned Human Class I Major Histocompatibility Complex Genes Into HLA Mutant Human Lymphoblastoid Cells", Mol. Cell. Biol., 1986, 6(4), 1074-1087. Shine, J. and Dalgarno, "Determinant of Cistron Specificity in Bacterial Ribosomes", Nature, 1975, 254, 34-38. Smith, G. et al., "Production of Human Beta Interferon in Insect Cells Infected with a Baculovirus Expression Vector", Mol. Cell. Biol., 1983, 3(12), 2156-2165. Smith, G. et al., "Modification and Secretion of Human Interleukin 2 Produced in Insect Cells by a Baculovirus Expression Vector", PNAS USA, 1985, 82, 8404-8408. Sriprakash, K.S. and MacAvoy, "A Gene for dnaB Like Protein in Chlamydial Plasmid", Nucl. Acids Res., 1987, 15(24), 10596. Sriprakash, K.S. and MacAvoy, "Characterization and Sequence of a Plasmid from the Trachoma Biovar of Chlamydia trachomatis", Plasmid, 1987, 18, 205-214. Somkuti, G.A. and Steinberg, "Genetic Transformation of Streptococcus thermophilus by Electroporation", Proc. 4th Eur. Congress on Biotechnology Jun. 14-19, 1987, vol. 1, Neijssel, O.M. et al. (eds.), Elsevier, Amsterdam, 1987. Steitz, J.A., "Genetic Signals and Nucleotide Sequence in Messenger RNA", in Biological Regulation and Development, vol. 1, Goldberger, R., ed., Plenum Press, New York, 1979. Stinchcomb, D. et al., "Centromeric DNA from Saccharomyces cerevisiae", J. Mol. Biol., 1982, 158, 157-179. Studier, F. William and Moffatt, "The Use of Bacteriophage T7 RNA Polymerase to Direct Selective High-Level Expression of Cloned Genes", J. Mol. Biol., 1986, 189, 113-130. Su, H. et al. "Protective efficacy of a parenterally administered MOMP-derived sythetic oligopeptide vaccine in a murine model of Chylamydia trachomatis gential tract
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (14)
Zaun Peter (Libertyville IL) Bouma Stanley R. (Grayslake IL) Gordon Julian (Lake Bluff IL) Kotlarik John J. (Vernon Hills IL), Apparatus and method for amplifying and detecting target nucleic acids.
Reinhartz Avraham (Rehovot ILX) Alajem Sarah (Kfar Hanagid ILX) Paper Thierry (Paris FRX) Herzberg Max (Satariya ILX), Apparatus and method for transport of nucleic acid sequences by capillary action on a solid support and detection of the.
Rabson Arthur L. (Chester NJ) Palmieri Thomas (Paramus NJ) Olson Douglas R. (Doylestown PA), Centrifuge vessel with coaxial waste chamber having cap to prevent waste fluid transfer from the chamber into the vessel.
Cole Francis X. (Stow MA) Sigillo Eric C. (Methuen MA) MacDonnell Paul C. (Bedford MA) Cicia Nancy J. (Wakefield MA), Porous strip form assay device method.
Finehout, Erin Jean; Puleo, Christopher Michael; Lenigk, Ralf; Nichols, Jason M.; Nelson, John, Detection of nucleic acid amplification in a porous substrate.
Reed, Michael W.; Nanassy, Oliver Z.; Haydock, Paul V.; Sharma, Nigel Rudra; Bardell, Ronald L.; Hargrave, Perry, Device and method for extraction and analysis of nucleic acids from biological samples.
Andreyev, Boris; Chu, Steven; Ciopyk, Brian; De La Zerda, Adam; Huang, Helen; Loney, Gregory; Swenson, David D., Devices and methods for molecular diagnostic testing.
Reed, Michael W.; Nanassy, Oliver Z.; Haydock, Paul V.; Sharma, Nigel Rudra; Bardell, Ronald L.; Hargrave, Perry, Devices and processes for nucleic acid extraction.
Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Poirier, Michael S.; Scott, Matthew J.; Taylor, Jason A., Instruments and methods for exposing a receptacle to multiple thermal zones.
Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Instruments and methods for mixing the contents of a detection chamber.
Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Instruments and methods for mixing the contents of a detection chamber.
Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Instruments and methods for mixing the contents of a detection chamber.
Breidenthal, Scott S.; Fan, Sara H.; Lee, Richard S.; Nelson, Norman C.; Scott, Matthew J.; Taylor, Jason A., Methods and instruments for processing a sample in a multi-chambered receptacle.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.