초록 ▼

Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal

Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be anti-ribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein.

대표청구항 ▼

Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal

Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be anti-ribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein. 1200, Ciliberto et al.; US-5869644, 19990200, Shortle et al.; US-5869696, 19990200, Reddy et al.; US-5874165, 19990200, Drumheller; US-5886104, 19990300, Pedersen et al.; US-6031074, 20000200, Saxinger Chem., 273(47):30863-30869 (1998). Wilson, R. et al., "2..2 Mb of contiguous nucleotide sequence from Chromosome III of C. Elegans," Nature, 368(6466):32-38 (1994). Yamaguchi, M. et al., "The primary structure of the rat guanylyl cyclase A/Atrial natriuretic peptide receptor gene," J. Biol. Chem., 265(33):20414-20420 (1990). GenBank Accession No. AA925138 for UI-R-A1-en-b-02-0-UI.s1 UI-R-A1 Rattus Norvegicus cDNA clone UI-R-A1-en-b-02-0-UI 3' similar to gi; 2809390; gb; AF031384; AFO31384 Rattus Norvegicus TWIK-related acid-sensitive K+ channel (Task) mRNA, complete cpds, mRNA sequence, Feb. 7, 1999. GenBank Accession No. AAA81455 for Similar to potassium channel proteins and to C. Elegans protein F22B7.7; Nov. 14, 1995. GenBank Accession No. AAA83199 for Weak similarity to a short region of putative potassium channel proteins; Dec. 12, 1995. GenBank Accession No. AAA96127 for Coded for by C. Elegans cDNA yk9g1.3; coded for by C. Elegans cDNA yk9g1.5;coded for by C. Elegans cDNA CEESU55F; Apr. 18, 1996. GenBank Accession No. AAB01688 for TWIK-1, Jun. 7, 1996. GenBank Accession No. AAB04567 for Weak similarity to a potassium channel protein, Jul. 22, 1996. GenBank Accession No. AAB52940 for Weak similarity to S. Cerevisiae outward-rectifier potassium channel TOK1, Apr. 21, 1997. GenBank Accession No. AAB54033 Unidentified protein, May 12, 1997. GenBank Accession No. AAB61602 for rabKCNK1, Jun. 18, 1997. GenBank Accession No. AAC16973 for TWIK-1 K+ channel, Sep. 29, 1998. GenBank Accession No. AAC32854 for Putative potassium channel subunit n2P17m1-2, Dec. 21, 1998. GenBank Accession No. AAC32855 for Putative potassium channel subunit n2P17m1-1, Dec. 21, 1998. GenBank Accession No. AAC32856 for Putative potassium channel subunit n2P16, Dec. 21, 1998. GenBank Accession No. AAC32857 for Putative potassium channel subunit n2P20 Dec. 21, 1998. GenBank Accession No. AAC32858 for Putative potassium channel subunit n2P17m2-2, Dec. 21, 1998. GenBank Accession No. AAC32859 for Putative potassium channel subunit n2P17m2-3. GenBank Accession No. AAC32860 for Putative potassium channel subunit n2P17m3, Dec. 12, 1998. GenBank Accession No. AAC32861 for Putative potassium channel subunit n2P18, Dec. 21, 1998. GenBank Accession No. AAC32862 for Putative potassium channel subunit n2P17m2-1, Dec. 21, 1998. GenBank Accession No. AAC32863 for Putative potassium channel subunit n2P38, Dec. 21, 1998. GenBank Accession No. AAC32865 for Putative potassium channel subunit n2P24, Dec. 21, 1998. GenBank Accession No. AAC39952 for Putative potassium channel subunit K+ channel, Feb. 2, 1999. GenBank Accession No. AAC40181 for TRAAK K+ channel subunit, Feb. 2, 1999. GenBank Accession No. AAC51777 for TWIK-related acid-sensitive K+ channel, Feb. 4, 1999. GenBank Accession No. AAC53005 for TREK-1 K+ channel subunit, Feb. 4, 1999. GenBank Accession No. AAC53367 for TWIK-related acid-sensitive K+ channel, Feb. 4, 1999. GenBank Accession No. AAC69250 for Two P domain potassium channel ORK1, Oct. 29, 1998. GenBank Accession No. AAC71141 for Proline-rich, Nov. 5, 1998. GenBank Accession No. AAC71151 for Contains similarity to outward-rectifier potassium channels, Nov. 5, 1998. GenBank Accession No. AAC79458 for Two-pore domain K+ channel; TASK-2, Nov. 17, 1998. GenBank Accession No. AAD01203 for Two-pore potassium channel TPKC1, Jul. 2, 1997. GenBank Accession No. AAD09336 for Putative potassium channel TWIK, Jan. 26, 1999. GenBank Accession No. AAD99337 for Putative potassium channel DP3, Jan. 26, 1999. GenBank Accession No. AAD09338 for Putative potassium channel DP4, Jan. 26, 1999. GenBank Accession No. AB008537 for Mus Musculus mRNA for cTBAK, complete cds, Feb. 13, 1999. GenBank Accession No. AB013345 for Mus Musculus mRNA for cTBAK, complete cds, May 21, 1998. GenBank Accession No. AC005035 for Homo sapiens BAC clone NH0353P23 from 2, complete sequence, Dec. 3, 1998. GenBank Accession No. AC005183 for Homo Sapiens Chr