초록 ▼

A system and method for manipulating magnetically responsive particles in a solution to separate nucleic acid molecules from cell components in a cell solution. The system and method employ a device capable of receiving a plurality of tubes, each of which contain respective sample and magnetically r

A system and method for manipulating magnetically responsive particles in a solution to separate nucleic acid molecules from cell components in a cell solution. The system and method employ a device capable of receiving a plurality of tubes, each of which contain respective sample and magnetically responsive particles. The device includes heating and cooling devices to facilitate a lysing step to release the nucleic acid molecules from the cells in the cell solution. The device further includes moveable magnets which can be moved proximate to and away from the tube to hold the magnetically responsive particles to which the nucleic acid molecules become bound, so that the molecule-bound particles can be separated from the remainder of the solution, and washed as appropriate. The system also employs an electromagnet which is capable of demagnetizing the particles to allow the particles to freely mix with solution, such as elution solutions which are used to unbind the molecules from the particles.

대표청구항 ▼

A system and method for manipulating magnetically responsive particles in a solution to separate nucleic acid molecules from cell components in a cell solution. The system and method employ a device capable of receiving a plurality of tubes, each of which contain respective sample and magnetically r

A system and method for manipulating magnetically responsive particles in a solution to separate nucleic acid molecules from cell components in a cell solution. The system and method employ a device capable of receiving a plurality of tubes, each of which contain respective sample and magnetically responsive particles. The device includes heating and cooling devices to facilitate a lysing step to release the nucleic acid molecules from the cells in the cell solution. The device further includes moveable magnets which can be moved proximate to and away from the tube to hold the magnetically responsive particles to which the nucleic acid molecules become bound, so that the molecule-bound particles can be separated from the remainder of the solution, and washed as appropriate. The system also employs an electromagnet which is capable of demagnetizing the particles to allow the particles to freely mix with solution, such as elution solutions which are used to unbind the molecules from the particles. r to said driving wheel for operator powered driving of said vehicle, said first transmission including no gears formed from aluminum or plastic, an assist motor for exerting a driving force to said vehicle through a second transmission, said second transmission including no gears formed from aluminum or plastic, a differential transmission comprised of at least one planetary transmission comprised of a sun gear element, a planer carrier element journalling a plurality of planet gears enmeshed with said sun gear element, and a ring gear element enmeshed with said sun gears, at least one of said planetary transmission elements being drivingly connected to said operator driven driving member and at least another of said planetary transmission elements being drivingly connected to said driving wheel for providing a signal indicative of change in phase between said operator driven driving member and said driving wheel, said differential transmission transmits no driving force between the operator driven driving member and the driving wheel and having its gears formed from a material selected from the group consisting of a plastic or aluminum, a phase change sensor associated with said differential transmission for providing a signal indicative of said change in phase, and control means responsive to the output signal of said phase change sensor for supplying an amount of power from said assist motor for assisting the driving of said vehicle said assist motor driving a wheel of the vehicle other than the driving wheel.