IPC분류정보
| 국가/구분 |
United States(US) Patent
등록
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| 국제특허분류(IPC7판) |
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| 출원번호 |
US-0444210
(1999-11-19)
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발명자
/ 주소 |
- Campos-Gonzalez, Roberto
- Hume, Steven Darrell
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| 출원인 / 주소 |
- Becton, Dickinson and Company
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| 대리인 / 주소 |
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| 인용정보 |
피인용 횟수 :
0 인용 특허 :
42 |
초록
▼
A method and device is disclosed for rapidly identifying a large number of proteins by placing a protein mixture in a sample chamber of an electrophoresis gel, and performing electrophoresis to separate the mixture by molecular weight, in a direction of separation, into a two-dimensional separation
A method and device is disclosed for rapidly identifying a large number of proteins by placing a protein mixture in a sample chamber of an electrophoresis gel, and performing electrophoresis to separate the mixture by molecular weight, in a direction of separation, into a two-dimensional separation pattern in the gel. The separation pattern is transferred to a membrane, such as a sheet of nitrocellulose, and a plate with a set of separate, side-by-side slots is then applied to the membrane. A different antibody mixture is introduced into each of the slots by perfusing each antibody mixture under pressure through the slots. The antibody mixture that is perfused through each slot recognizes several different proteins of sufficiently different molecular weights that different protein bands can be resolved by the antibody mixture in each slot. Proteins of similar molecular weights are recognized by antibody mixtures perfused through different slots, such that otherwise overlapping protein bands are detected in different lanes. The positions of protein bands in each of the lanes are correlated with expected positions of proteins recognized by the mixture for that lane, and the presence of a band is correlated with the presence of the corresponding protein in the mixture. It is emphasized that this abstract is provided to comply with the rules requiring an abstract which will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret of limit the scope or meaning of the claims.
대표청구항
▼
A method and device is disclosed for rapidly identifying a large number of proteins by placing a protein mixture in a sample chamber of an electrophoresis gel, and performing electrophoresis to separate the mixture by molecular weight, in a direction of separation, into a two-dimensional separation
A method and device is disclosed for rapidly identifying a large number of proteins by placing a protein mixture in a sample chamber of an electrophoresis gel, and performing electrophoresis to separate the mixture by molecular weight, in a direction of separation, into a two-dimensional separation pattern in the gel. The separation pattern is transferred to a membrane, such as a sheet of nitrocellulose, and a plate with a set of separate, side-by-side slots is then applied to the membrane. A different antibody mixture is introduced into each of the slots by perfusing each antibody mixture under pressure through the slots. The antibody mixture that is perfused through each slot recognizes several different proteins of sufficiently different molecular weights that different protein bands can be resolved by the antibody mixture in each slot. Proteins of similar molecular weights are recognized by antibody mixtures perfused through different slots, such that otherwise overlapping protein bands are detected in different lanes. The positions of protein bands in each of the lanes are correlated with expected positions of proteins recognized by the mixture for that lane, and the presence of a band is correlated with the presence of the corresponding protein in the mixture. It is emphasized that this abstract is provided to comply with the rules requiring an abstract which will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret of limit the scope or meaning of the claims. et al.; US-5580575, 19961200, Unger et al.; US-5580731, 19961200, Chang et al.; US-5583020, 19961200, Sullivan; US-5585481, 19961200, Arnold, Jr. et al.; US-5587361, 19961200, Cook et al.; US-5587371, 19961200, Sessler et al.; US-5587469, 19961200, Cook et al.; US-5591584, 19970100, Chang et al.; US-5591721, 19970100, Agrawal et al.; US-5591722, 19970100, Montgomery et al.; US-5594121, 19970100, Froehler et al.; US-5595726, 19970100, Magda et al.; US-5595756, 19970100, Bally et al.; US-5596086, 19970100, Matteucci et al.; US-5596091, 19970100, Switzer; US-5597696, 19970100, Linn et al.; US-5597909, 19970100, Urdea et al.; US-5599923, 19970200, Sessler et al.; US-5599928, 19970200, Hemmi et al.; US-5602240, 19970200, De Mesmaeker et al.; US-5608046, 19970300, Cook et al.; US-5610289, 19970300, Cook et al.; US-5610300, 19970300, Altmann et al.; US-5614617, 19970300, Cook et al.; US-5618704, 19970400, Sanghvi et al.; US-5623065, 19970400, Cook et al.; US-5623070, 19970400, Cook et al.; US-5625050, 19970400, Beaton et al.; US-5627053, 19970500, Usman et al.; US-5633360, 19970500, Bischofberger et al.; US-5639873, 19970600, Barascut et al.; US-5646265, 19970700, McGee; US-5652355, 19970700, Metelev et al.; US-5652356, 19970700, Agrawal; US-5658873, 19970800, Bertsch-Frank et al.; US-5663312, 19970900, Chaturvedula; US-5670633, 19970900, Cook et al.; US-5677437, 19971000, Teng et al.; US-5677439, 19971000, Weis et al.; US-5681941, 19971000, Cook et al.; US-5688941, 19971100, Cook et al.; US-5700920, 19971200, Altmann et al.; US-5700922, 19971200, Cook; US-5705188, 19980100, Junichi et al.; US-5714331, 19980200, Buchardt et al.; US-5719262, 19980200, Buchardt et al.; US-5750692, 19980500, Cook et al.; US-5824544, 19981000, Arlmentano et al.; US-5830730, 19981100, German et al.; US-5872154, 19990200, Wilson et al.; US-5885808, 19990300, Spooner et al.; US-5981225, 19991100, Kuchanek et al.; US-5994069, 19991100, Hall et al.; US-5994106, 19991100, Kovesdi et al.; US-5994128, 19991100, Fallaux et al.; US-5994132, 19991100, Chamberlain et al.; US-5994529, 19991100, Feuerstein et al.; US-6001557, 19991200, Wilson et al.; US-6019978, 20000200, Ertl et al.; US-6033908, 20000300, Bout et al.; US-6287835, 20010900, Croteau et al.
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