Sugarcane ubi9 gene promoter sequence and methods of use thereof
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-005/04
C12N-015/82
C12N-015/90
A01H-005/00
출원번호
US-0693467
(2000-10-20)
발명자
/ 주소
Albert, Henrik H.
Wei, Hairong
출원인 / 주소
The United States of America as represented by the Secretary of Agriculture, University of Hawaii
대리인 / 주소
Medlen & Carroll, LLP
인용정보
피인용 횟수 :
1인용 특허 :
17
초록▼
The invention relates to the nucleotide sequence from the sugarcane ubi9 polyubiquitin gene promoter, which is capable of directing constitutive expression of a nucleic acid sequence of interest that is operably linked to it. The sugarcane ubi9 promoter is useful in regulating expression of a nuclei
The invention relates to the nucleotide sequence from the sugarcane ubi9 polyubiquitin gene promoter, which is capable of directing constitutive expression of a nucleic acid sequence of interest that is operably linked to it. The sugarcane ubi9 promoter is useful in regulating expression of a nucleic acid sequence of interest in monocotyledonous and dicotyledonous plants.
대표청구항▼
1. A transgenic plant cell comprising a promoter comprising SEQ ID NO:10, wherein said promoter is operably linked to a nucleic acid sequence of interest, and said plant cell is selected from sugar cane, tobacco, sorghum, pineapple, rice, maize, tomato, soybean, banana, and garlic. 2. The transgenic
1. A transgenic plant cell comprising a promoter comprising SEQ ID NO:10, wherein said promoter is operably linked to a nucleic acid sequence of interest, and said plant cell is selected from sugar cane, tobacco, sorghum, pineapple, rice, maize, tomato, soybean, banana, and garlic. 2. The transgenic plant cell of claim 1, wherein said plant cell is from garlic. 3. The transgenic plant cell of claim 1, wherein said plant cell is a leaf cell, root cell, fruit cell, or embryonic cell. 4. The transgenic plant cell of claim 1, wherein said plant cell is transiently transformed. 5. The transgenic plant cell of claim 1, wherein said plant cell is stably transformed. 6. A method for expressing a nucleic acid sequence of interest in a plant cell selected from sugar cane, tobacco, sorghum, pineapple, rice, maize, tomato, soybean, banana, and garlic, comprising: a) providing: i) said plant cell; ii) a nucleic acid sequence of interest; and iii) a promoter comprising SEQ ID NO: 10; b) operably linking said nucleic acid sequence of interest to said promoter to produce a transgene; and c) introducing said transgene into said plant cell to produce a transgenic plant cell under conditions such that said nucleic acid sequence of interest is expressed in said transgenic plant cell. 7. The method of claim 6, further comprising d) identifying said transgenic plant cell. 8. The method of claim 6, further comprising d) regenerating transgenic plant tissue from said transgenic plant cell. 9. The method of claim 6, further comprising d) regenerating a transgenic plant from said transgenic plant cell. 10. The method of claim 6, wherein said plant cell is from garlic. 11. The method of claim 6, wherein said plant cell is a leaf cell, root cell, fruit cell, or embryonic cell. 12. The method of claim 6, wherein said transgenic plant cell is transiently transformed. 13. The method of claim 6, wherein said transgenic plant cell is stably transformed.
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이 특허에 인용된 특허 (17)
Grierson Donald (Loughbrough GB2) Hamilton Andrew J. (Nottingham GB2) Lycett Grantley W. (Loughbrough GB2), Antisense constructs derived from pTOM13 plants and plant cells with reduced ethylene evolution.
Shah Dilip M. (Creve Coeur MO) Rogers Stephen G. (Chesterfield MO) Horsch Robert B. (both St. Louis MO) Fraley Robert T. (both St. Louis MO), Glyphosate-resistant plants.
Christou Paul (Madison WI) McCabe Dennis (Middleton WI) Swain William F. (Madison WI) Barton Kenneth A. (Middleton WI), Particle-mediated transformation of soybean plants and lines.
Quail Peter H. (Richmond CA) Christensen Alan H. (Albany CA) Hershey Howard P. (West Chester PA) Sharrock Robert A. (El Cerrito CA) Sullivan Thomas D. (Madison WI), Plant ubiquitin promoter system.
Quail Peter H. (Richmond CA) Christensen Alan H. (Albany CA) Hershey Howard P. (West Chester PA) Sharrock Robert A. (El Cerrito CA) Sullivan Thomas D. (Madsion WI), Plant ubiquitin promoter system.
Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Gelfand David H. (Oakland CA) Horn Glenn (Emeryville CA) Saiki Randall K. (Richmond CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme.
Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
Schilperoort Robbert A. (Anthonie Duycklaan 10c ; 2334 CD Leiden NLX) Hoekema Andreas (Boerhaavelaan 114 ; 2334 ET Leiden NLX) Hooykaas Paul J. J. (Condorstraat 126 ; 2317 AW Leiden NLX), Process for the incorporation of foreign dna into the genome of dicotyledonous plants.
Offringa Remko (Leiden NLX) De Groot Marcellus J. A. (Utrecht NLX) Hooykaas Paul J. J. (Oegstgeest NLX) Van Den Elzen Petrus J. M. (Voorhout NLX), Process for the site-directed integration of DNA into the genome of plants.
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