초록 ▼

A method and apparatus for aseptic biological production of cells and/or microorganisms are provided. The apparatus includes a support housing having an interior chamber, a disposable flexible sterile plastic liner lining the interior chamber and a head plate attached to the liner forming a sealed c

A method and apparatus for aseptic biological production of cells and/or microorganisms are provided. The apparatus includes a support housing having an interior chamber, a disposable flexible sterile plastic liner lining the interior chamber and a head plate attached to the liner forming a sealed chamber with the liner to form a reservoir. After use the liner can be thrown away and the apparatus can be reused with a new liner. In this way, the apparatus simplifies cleaning and ensuring validation required by pharmaceutical and food industry standards. Aerating fluid is introduced during culturing of cell or microorganisms in the reservoir. A growth characteristic can be used to vary flow rate and/or composition of the aerating fluid. Another reservoir may be used for circulation between two reservoirs.

대표청구항 ▼

A method and apparatus for aseptic biological production of cells and/or microorganisms are provided. The apparatus includes a support housing having an interior chamber, a disposable flexible sterile plastic liner lining the interior chamber and a head plate attached to the liner forming a sealed c

A method and apparatus for aseptic biological production of cells and/or microorganisms are provided. The apparatus includes a support housing having an interior chamber, a disposable flexible sterile plastic liner lining the interior chamber and a head plate attached to the liner forming a sealed chamber with the liner to form a reservoir. After use the liner can be thrown away and the apparatus can be reused with a new liner. In this way, the apparatus simplifies cleaning and ensuring validation required by pharmaceutical and food industry standards. Aerating fluid is introduced during culturing of cell or microorganisms in the reservoir. A growth characteristic can be used to vary flow rate and/or composition of the aerating fluid. Another reservoir may be used for circulation between two reservoirs. ion", Molecular Microbiology, 24, pp. 879-880 (1997). G. Novelli et al., "Study of the Effects on DNA of Electromagnetic Fields Using Clamped Homogeneous Electric Field Gel Electrophoresis", Biomedicine & Pharmacotherapy, 45, pp. 451-454 (1991). J.L. Phillips, "Effects of Electromagnetic Field Exposure on Gene Transcription", Journal of Cellular Biochemistry, 51, pp. 381-386 (1993). V. Romano-Spica et al., "Ets1 Oncogene Induction by ELF-Modulated 50 MHz Radiofrequency Electromagnetic Field", Bioelectromagnetics, 21, pp. 8-18 (2000). J.E. Trosko, "Human Health Consequences of Environmentally-Modulated Gene Expression: Potential Roles of ELF-EMF Induced Epigenetic Versus Mutagenic Mechanisms of Disease", Bioelectromagnetics, 21, pp. 402-406 (2000). C. Ventura et al., "Elf-pulsed Magnetic Fields Modulate Opioid Peptide Gene Expression in Myocardial Cells", Cardiovascular Research, 45, pp. 1054-1064 (2000). A.M. Woodward et al., "Genetic Programming as an Analytical Tool for Non-linear Dielectric Spectroscopy", Bioelectrochemistry and Bioenergetics, 48, pp. 389-396 (1999). T. Yonetani et al., "Electromagnetic Properties of Hemoproteins", The Journal of Biological Chemistry, 247, pp. 2447-2455 (1972). L. Zhang et al., "Electrostimulation of the Dehydrogenase System of Yeast by Alternating Currents", Bioelectrochemistry and Bioenergetics, 28, pp. 341-353 (1992). Binninger, D. M. et al., "Effects of 60Hz AC magnetic fields-on gene expression following exposure over multiple cell generations using Saccharomyces cerevisiae", Bioelectrochemistry and Bioenergetics, 43(1): 83-89 (1997). Deguchi, T. et al., "Nylon biodegradation by lignin-degrading fungi", Applied and Environmental Microbiology, 63(1): 329-331 (1997). Pichko, V. B. et al., "Electromagnetic stimulation of productivity of microorganisms and its mechanisms", Prikladnaya Biokhimiya I Mikrobiologiya, 32(4): 468-472 (1996). Ponne, C. T. et al., "Interaction of electromagnetic energy with biological material--relation to food processing", Radiation Physics and Chemistry, 45(4): 591-607 (1995). Van Rensburg, P. et al., "Engineering yeast for efficient cellulose degradation", Yeast, 14(1): 67-76 (1998). "Saccharomyces cerevisiae Meyen ex Hansen", China Catalogue of Cultures/China Committee of Culture Collection for Microorganisms (CCCCM), "www.im.ac.cn/database/YEAST/y122.htm", Apr. 24, 1996, retrieved on Nov. 27, 2002.