IPC분류정보
| 국가/구분 |
United States(US) Patent
등록
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| 국제특허분류(IPC7판) |
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| 출원번호 |
US-0960264
(2001-09-20)
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발명자
/ 주소 |
- Berenson, Ronald
- Law, Che
- Bonyhadi, Mark
- Saund, Narinder
- Craig, Stewart
- Hardwick, Alan
- Kalamasz, Dale
- McMillen, David
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| 출원인 / 주소 |
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| 대리인 / 주소 |
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| 인용정보 |
피인용 횟수 :
57 인용 특허 :
24 |
초록
▼
The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yieldi
The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.
대표청구항
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1. A device adapted for the magnetic attraction of particles in a cell culture comprising a cell culture vessel adapted for the culture of human cells and a magnet or a magnetizable element, wherein said magnet or magnetizable element is positioned within a wall of said culture vessel or external to
1. A device adapted for the magnetic attraction of particles in a cell culture comprising a cell culture vessel adapted for the culture of human cells and a magnet or a magnetizable element, wherein said magnet or magnetizable element is positioned within a wall of said culture vessel or external to said culture vessel so as to allow a magnetic field emanating therefrom to pass through said culture vessel. 2. The device of claim 1, wherein said magnet or magnetizable element is an electromagnet. 3. The device of claim 1, wherein said human cells are lymphocytes. 4. The device of claim 1, wherein said culture vessel is a flask or a bag. 5. A method for inducing aggregation of cells or cell surface markers comprising:(a) providing a population of cells having a target cell surface molecule; and(b) providing a device, wherein said device is adapted for the magnetic attraction of particles in a cell culture and wherein said device comprisesa cell culture vessel adapted for the culture of human cells, anda magnet or a magnetizable element, wherein said magnet or magnetizable element is positioned so as to allow a magnetic field emanating therefrom to pass through said culture vessel; andwherein said cells are contained within said culture vessel; said culture vessel contains a solid surface having attached thereto a ligand for at least one target cell surface molecule; and(c) applying a magnetic field through said magnet or magnetizable element of said device,thereby inducing aggregation of said cells or cell surface markers. 6. The method of claim 5, wherein said aggregation induces target cell signal transduction. 7. The method of claim 5, wherein said solid surface is selected from the group consisting of a plate, a bag, a dish, a rod, a pellet, a fiber, a microsphere, and a bead. 8. The method of claim 7, wherein said solid surface is a bead or a microsphere. 9. The method of claim 7, wherein said solid surface is paramagnetic. 10. The method of claim 5, wherein said cell population comprises lymphocytes. 11. The method of claim 5, wherein said ligand is selected from the group consisting of an antibody, a natural ligand, and a synthetic ligand. 12. The method of claim 5, wherein said ligand comprises an antibody, a peptide, a polypeptide, a growth factor, a cytokine, or a chemokine. 13. The method of claim 5, wherein said receptor binding leads to downregulation or suppression of a cellular event. 14. The method of claim 5, wherein said receptor binding leads to upregulation or activation of a cellular event.
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