Plant long chain fatty acid biosynthetic enzyme
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01H-005/00
C12N-015/82
출원번호
US-0787962
(2000-07-21)
국제출원번호
PCT/CA00/00860
(2000-07-21)
국제공개번호
WO01/07586
(2001-02-01)
발명자
/ 주소
Kunst, Ljerka
Clemens, Sabine
출원인 / 주소
The University of British Columbia
대리인 / 주소
Klarquist Sparkman LLP
인용정보
피인용 횟수 :
1인용 특허 :
12
초록▼
In various aspects, the present invention provides nucleic acid sequence encoding all or part of a new plant long chain fatty acid condensing enzyme (a fatty acid elongase), designated herein as KCS2 (for beta-ketoacyl-coenzyme A synthase 2). In some embodiments, KCS2 may mediate the biosynthesis of
In various aspects, the present invention provides nucleic acid sequence encoding all or part of a new plant long chain fatty acid condensing enzyme (a fatty acid elongase), designated herein as KCS2 (for beta-ketoacyl-coenzyme A synthase 2). In some embodiments, KCS2 may mediate the biosynthesis of C18, C20, C22 and C24 fatty acids. The activity of the enzyme is typically characterized by two carbon (malonyl-CoA) additions to C16, C18, C20 and C22 moieties (C16-C22 acyl CoA molecules), i.e. condensation of malonyl-CoA with a C16, C18, C20 or C22 acyl-CoA. The fatty acids produced by the enzyme may for example be saturated 18:0, 20:0, 22:0 and 24:0 fatty acids. The invention includes recombinant nucleic acid molecules comprising a heterologous nucleic acid coding sequence encoding the plant long chain fatty acid condensing enzyme.
대표청구항▼
1. A recombinant nucleic acid molecule comprising a heterologous polynucleotide selected from the group consisting of:(a) a nucleic acid coding sequence having at least 95% sequence identity to the Arabidopsis KCS2 coding sequence shown at position 1046-2509 of SEQ ID NO: 1, when optimally aligned,
1. A recombinant nucleic acid molecule comprising a heterologous polynucleotide selected from the group consisting of:(a) a nucleic acid coding sequence having at least 95% sequence identity to the Arabidopsis KCS2 coding sequence shown at position 1046-2509 of SEQ ID NO: 1, when optimally aligned, wherein the sequence encodes a plant very long chain fatty acid condensing enzyme that catalyses condensation of malonyl-CoA with a C16, C18, C20, or C22 acyl-CoA; and(b) a complementary nucleic acid sequence fully complementary to the nucleic acid sequence of (a). 2. The recombinant nucleic acid molecule of claim 1 wherein the nucleic acid coding sequence is the Arabidopsis KCS2 coding sequence shown at position 1046-2509 of SEQ ID NO: 1. 3. The recombinant nucleic acid molecule of claim 1 wherein the nucleic acid coding sequence hybridizes under stringent conditions to the complement of the Arabidopsis KCS2 coding sequence shown at position 1046-2509 of SEQ ID NO: 1. 4. A transgenic plant comprising the recombinant nucleic acid molecule of claim 1. 5. A part of a transgenic plant comprising the recombinant nucleic acid molecule of claim 1. 6. The transgenic plant of claim 4, wherein the transgenic plant has a modified phenotype compared to a non-transgenic plant of the same species. 7. The transgenic plant of claim 4, wherein the plant is a monocot. 8. The transgenic plant of claim 4, wherein the plant is a dicot. 9. The transgenic plant of claim 4, wherein the plant is a species of one of the Cruciferae family, Compositae family, Palmae family, or Leguminosae family. 10. The transgenic plant of claim 4, wherein the plant is selected from the group consisting of: canola, rapeseed ( Brassica spp.), crambe ( Crambe spp.), honesty ( Lunaria spp.) lesquerella ( Lesquerela spp.), sunflower ( Helianthus spp.), safflower ( Carthamus spp.), niger ( Guizotia spp.), palm ( Elaeis spp.), coconut ( Cocos spp.), peanut ( arachis spp.), soybean ( Glycine spp.), ( Zea spp.), cotton ( Gossypium sp.), jojoba ( Simmondsia sp.), flax ( Linum sp.), sesame ( Sesamum spp.), castor bean ( Ricinus spp.), olive ( Olea spp.), poppy ( Papaver spp.), spurge ( Euphorbia spp.), meadowfoam ( Limnanthes spp.), mustard ( Sinapis spp.) and cuphea ( Cuphea spp.). 11. A transgenic seed comprising the recombinant nucleic acid molecule of claim 1. 12. The transgenic seed of claim 11, wherein the seed is for a plant of the species of one of the Cruciferae family, Compositae family, Palmae family, or Leguminosae family. 13. The transgenic seed of claim 11, wherein the seed is for a plant selected from the group consisting of: canola, rapeseed ( Brassica spp.), crambe ( Crambe spp.), honesty ( Lunaria spp.) lesquerella ( Lesquerela spp.), sunflower ( Helianthus spp.), safflower ( Carthamus spp.), niger ( Guizotia spp.), palm ( Elaeis spp.), coconut ( Cocos spp.), peanut ( arachis spp.), soybean ( Glycine spp.), ( Zea spp.), cotton ( Gossypium sp.), jojoba ( Simmondsia sp.), flax ( Linum sp.), sesame ( Sesamum spp.), castor bean ( Ricinus spp.), olive ( Olea spp.), poppy ( Papaver spp.), spurge ( Euphorbia spp.), meadowfoam ( Limnanthes spp.), mustard ( Sinapis spp.) and cuphea ( Cuphea spp.). 14. A transgenic cell comprising the recombinant nucleic acid of claim 1. 15. The transgenic cell of claim 14, wherein the cell is a non-human cell. 16. The transgenic cell of claim 14, wherein the cell is a plant cell. 17. A recombinant expression vector comprising the recombinant nucleic acid molecule of claim 1. 18. A recombinant expression cassette comprising the recombinant nucleic acid molecule of claim 1 operably linked to a promoter. 19. An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:(a) a nucleic acid coding sequence having at least 95% sequence identity to the Arabidopsis KCS2 coding sequence shown at position 1046-2509 of SEQ ID NO: 1, when optimally aligned, wherein th e sequence encodes a plant very long chain fatty acid condensing enzyme that catalyses condensation of malonyl-CoA with a C16, C18, C20, or C22 acyl-CoA; and(b) a complementary nucleic acid sequence fully complementary to the nucleic acid sequence of (a). 20. The isolated nucleic acid molecule of claim 19 wherein the nucleic acid coding sequence is the Arabidopsis KCS2 coding sequence shown at position 1046-2509 of SEQ ID NO: 1. 21. The isolated nucleic acid molecule of claim 19 wherein the nucleic acid coding sequence hybridizes under stringent conditions to the complement of the Arabidopsis KCS2 coding sequence shown at position 1046-2509 of SEQ ID NO: 1. 22. A method of producing a transgenic plant, the method comprising introducing into the plant the isolated nucleic acid molecule of claim 19. 23. A progeny plant produced by sexual or asexual propagation of the transgenic plant produced by the method of claim 22 or produced by propagation of the progeny plant, wherein the progeny plant comprises the nucleic acid molecule. 24. A transgenic plant comprising the isolated nucleic acid molecule of claim 19. 25. A part of a transgenic plant comprising the isolated nucleic acid molecule of claim 19. 26. The transgenic plant of claim 24, wherein the transgenic plant has a modified phenotype compared to a non-transgenic plant of the same species. 27. The transgenic plant of claim 26, wherein the plant is a monocot. 28. The transgenic plant of claim 26, wherein the plant is a dicot. 29. The transgenic plant of claim 26, wherein the plant is a species of one of the Cruciferae family, Compositae family, Palmae family, or Leguminosae family. 30. The transgenic plant of claim 26, wherein the plant is selected from the group consisting of: canola, rapeseed ( Brassica spp.), crambe ( Crambe spp.), honesty ( Lunaria spp.) lesquerella ( Lesquerela spp.), sunflower ( Helianthus spp.), safflower ( Carthamus spp.), niger ( Guizotia spp.), palm ( Elaeis spp.), coconut ( Cocos spp.), peanut ( arachis spp.), soybean ( Glycine spp.), ( Zea spp.), cotton ( Gossypium sp.), jojoba ( Simmondsia sp.), flax ( Linum sp.), sesame ( Sesamum spp.), castor bean ( Ricinus spp.), olive ( Olea spp.), poppy ( Papaver spp.), spurge ( Euphorbia spp.), meadowfoam ( Limnanthes spp.), mustard ( Sinapis spp.) and cuphea ( Cuphea spp.). 31. A transgenic seed comprising the isolated nucleic acid molecule of claim 19. 32. The transgenic seed of claim 31, wherein the seed is for a plant of a species of one of the Cruciferae family, Compositae family, Palmae family, or Leguminosae family. 33. The transgenic seed of claim 31, wherein the seed is for a plant selected from the group consisting of: canola, rapeseed ( Brassica spp.), crambe ( Crambe spp.), honesty ( Lunaria spp.) lesquerella ( Lesquerela spp.), sunflower ( Helianthus spp.), safflower ( Carthamus spp.), niger ( Guizotia spp.), palm ( Elaeis spp.), coconut ( Cocos spp.), peanut ( arachis spp.), soybean ( Glycine spp.), ( Zea spp.), cotton ( Gossypium sp.), jojoba ( Simmondsia sp.), flax ( Linum sp.), sesame ( Sesamum spp.), castor bean ( Ricinus spp.), olive ( Olea spp.), poppy ( Papaver spp.), spurge ( Euphorbia spp.), meadowfoam ( Limnanthes spp.), mustard ( Sinapis spp.) and cuphea ( Cuphea spp.). 34. A transgenic cell comprising the isolated nucleic acid molecule of claim 19. 35. The transgenic cell of claim 34, wherein the cell in non-human cell. 36. The transgenic cell of claim 34, wherein the cell is a plant cell. 37. A recombinant expression vector comprising the isolated nucleic acid molecule of claim 19. 38. A recombinant expression cassette comprising the isolated nucleic acid molecule of claim 19 operably linked to a promoter.
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이 특허에 인용된 특허 (12)
McCabe Dennis E. (Middleton WI) Martinell Brian J. (Madison WI), Apparatus for genetic transformation.
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Schilperoort Robbert A. (Leiden NLX) Hoekema Andreas (Leiden NLX), Process for the incorporation of foreign DNA into the genome of dicotyledonous plants.
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