초록

This invention relates to a method for extracting, fractionating and purifying polyphenolic compounds originating from plants, wherein the purification is performed by adsorption on an adsorbing styrene-divinyl benzene resin, followed by the elution of the polyphenolic compounds.

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1. A method of obtaining a purified extract comprising polyphenolic compounds from a plant comprising polyphenolic compounds, the method comprising:extracting a plant material in order to obtain a raw plant material extract comprising polyphenolic compounds, wherein extracting comprises:(a) heating

1. A method of obtaining a purified extract comprising polyphenolic compounds from a plant comprising polyphenolic compounds, the method comprising:extracting a plant material in order to obtain a raw plant material extract comprising polyphenolic compounds, wherein extracting comprises:(a) heating the plant material: and(b) submitting the plant material of step (a) to a vacuum:adsorbing the polyphenolic compounds contained in the extract on an adsorbing styrene-divinyl benzene resin that has a high adsorption performance and a high elution performance;eluting the polyphenolic compounds from the resin to obtain a purified extract comprising the polyphenolic compounds, andconcentrating the purified extract;wherein the resin is further defined as having pores with an average size in the range from 50 to 110 angstroms, a surface area equal to or higher than 800 m 2 /g and a pore volume higher than 1 ml/g; and the plant material is all or part of a leafy plant of the Compositae family or a bulb plant of the Liliaceae family. 2. The method of claim 1, wherein the resin is further defined as having pores with an average size in the range 60 to 100 angstroms. 3. The method of claim 1, wherein the resin is further defined as having pores with an average size in the range 80 to 110 angstroms. 4. The method of claim 3, wherein the resin is further defined as having pores with an average size in the range 88 to 92 angstroms. 5. The method of claim 4, wherein the resin is further defined as having pores with an average size of about 90 angstroms. 6. The method of claim 1, wherein the resin is further defined as having a surface area equal to or higher than 880 m 2 /g. 7. The method of claim 6, wherein the resin is further defined as having a surface area equal to or higher than 1000 m 2 /g. 8. The method of claim 7, wherein the resin is further defined as having a surface area equal to or higher than 1200 m 2 /g. 9. The method of claim 1, wherein the resin is further defined as having a pore volume equal to or higher than 1.4 ml/g. 10. The method of claim 9, wherein the resin is further defined as having a pore volume equal to or higher than 2 ml/g. 11. The method of claim 10, wherein the resin is further defined as having a pore volume equal to or higher than 2.4 ml/g. 12. The method of claim 1, wherein the resin is further defined as having the following physical features:pores with an average size in the range from 80 to 110 angstroms;a surface area equal to or higher than 1000 m 2 /g; anda pore volume equal to or higher than 2 ml/g. 13. The method of claim 12, wherein the resin is further defined as having the following physical features:pores with an average size of about 90 angstroms;a surface area equal to or higher 1200 m 2 /g; anda pore volume equal to or higher than 2.4 ml/g. 14. The method of claim 1, wherein the resin has an adsorption rate of polyphenolic compounds equal to or higher than 80% and an elution rate of polyphenolic compounds equal to or higher than 60%. 15. The method of claim 1, wherein the resin has the formula (I): 16. The method of claim 1, wherein submitting the plant material of step (a) to a vacuum produces a vapour, and wherein the vapour is condensed. 17. The method of claim 1, wherein the heating of step (a) occurs for less than fifteen minutes and without maceration. 18. The method of claim 1, wherein the heating of step (a) occurs in the absence of oxygen. 19. The method of claim 1, wherein the heating of step (a) is done with a condensing vapour. 20. The method of claim 1, wherein the heating of step (a) comprises circulating heated exudation juice or condensed vapour from a vacuum step to heat the plant material. 21. The method of claim 1, wherein the heating of step (a) comprises direct supply of heat to the plant material from a heat exchanger. 22. The method of claim 21, wherein the heat exchanger is a scraped surface heat exchanger. 23. The method of claim 1, wherein extracting is carried out in a continuous mode. 24. The method of claim 1, wherein extracting is carried out in a batch mode. 25. The method of claim 1, wherein eluting comprises use of a food solvent eluent. 26. The method of claim 25, wherein the food solvent eluent is ethanol or methanol. 27. The method of claim 1, wherein the purified extract has a polyphenolic compound content equal to or higher than 30% by weight. 28. The method of claim 1, further comprising drying the purified extract. 29. The method of claim 28, wherein the purified extract is spray-dried. 30. The method of claim 28, wherein the purified extract is freeze-dried. 31. The method of claim 1, wherein the purified extract is in a dry form. 32. The method of claim 31, wherein the dry form is further defined as a powder, granule, or ball form. 33. The method of claim 1, wherein the purified extract is in a liquid form. 34. The method of claim 1, wherein the plant is a leafy plant of the Compositae family selected from the group consisting of lettuce, endive, curly endive, and escarole. 35. The method of claim 34, wherein the plant is a lettuce selected from the group consisting of cos lettuce, batavia, and iceberg lettuce. 36. The method of claim 1, wherein the plant is a bulb plant of the Liliaceae family selected from the group consisting of onion, garlic, leek, and shallot. 37. The method claim 1, further comprising placing the purified extract in a cosmetic, food, or pharmaceutical. 38. The method of claim 1, wherein the resin is further defined as having:a pore volume equal to or higher than 1.4 ml/g;pores with an average size in the range 60 to 100 angstroms; anda surface area equal to or higher than 880 m 2 /g. 39. The method of claim 1, wherein the heating the plant material comprises heating at a temperature ranging from about 90° C. to about 105° C. 40. The method of claim 1, wherein submitting the plant material of step (a) to a vacuum comprises vacuuming at a pressure ranging from about 10 3 to about 2×10 4 Pa.