IPC분류정보
국가/구분 |
United States(US) Patent
등록
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국제특허분류(IPC7판) |
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출원번호 |
US-0791610
(2001-02-26)
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우선권정보 |
JP-0054668 (2000-02-29); JP-0054669 (2000-02-29); JP-0294634 (2000-09-27) |
발명자
/ 주소 |
- Honma, Tsutomu
- Sugawa, Etsuko
- Yano, Tetsuya
- Kobayashi, Shin
- Imamura, Takeshi
- Kenmoku, Takashi
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출원인 / 주소 |
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대리인 / 주소 |
Fitzpatrick, Cella, Harper &
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인용정보 |
피인용 횟수 :
12 인용 특허 :
5 |
초록
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Microorganisms capable of producing novel polyalkanoates having 3-hydroxybenzoylalkanoic acids as monomer units by utilizing benzoylalkanoic acids as starting materials are cultured in media containing benzoylalkanoic acids and saccharides, and the polyhydroxyalkanoates produced in the culture cells
Microorganisms capable of producing novel polyalkanoates having 3-hydroxybenzoylalkanoic acids as monomer units by utilizing benzoylalkanoic acids as starting materials are cultured in media containing benzoylalkanoic acids and saccharides, and the polyhydroxyalkanoates produced in the culture cells are extracted and recovered.
대표청구항
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1. A polyhydroxyalkanoate having a monomer unit composition represented by the following Formula [1]:AxB(1?x)??[1]wherein A is at least one monomer unit represented by the following Chemical Formula [2]: ?where n is an integer of 1 to 8; and B is at least one selected from monomer units represented
1. A polyhydroxyalkanoate having a monomer unit composition represented by the following Formula [1]:AxB(1?x)??[1]wherein A is at least one monomer unit represented by the following Chemical Formula [2]: ?where n is an integer of 1 to 8; and B is at least one selected from monomer units represented by the following Chemical Formula [3]: ?where p is an integer of 0 to 10, and monomer units represented by the following Chemical Formula [4]: ?where q is 3 or 5; and x is not less than 0.01 and less than 1. 2. The polyhydroxyalkanoate according to claim 1, wherein the one or more monomer units include a monomer unit represented by the Chemical Formula [5]: 3. The polyhydroxyalkanoate according to claim 1, wherein the one or more monomer units include a monomer unit represented by the Chemical Formula [6]: 4. The polyhydroxyalkanoate according to claim 1, wherein the one or more monomer units include a monomer unit represented by the Chemical Formula [7]: 5. The polyhydroxyalkanoate according to claim 1, wherein the one or more monomer units include a monomer unit represented by the Chemical Formula [8]: 6. The polyhydroxyalkanoate according to claim 1, wherein the one or more monomer units include a monomer unit represented by the Chemical Formula [9]: 7. The polyhydroxyalkanoate according to claim 1, wherein the monomer units include a monomer unit represented by the Chemical Formula [6]: and a monomer unit represented by the Chemical Formula [8]: 8. The polyhydroxyalkanoate according to claim 1, wherein the monomer units include a monomer unit represented by the Chemical Formula [7]: and a monomer unit represented by the Chemical Formula [9]: 9. The polyhydroxyalkanoate according to claim 1, wherein the polyhydroxyalkanoate has a number-average molecular weight of 10,000 to 1,000,000.10. A method for producing a polyhydroxyalkanoate, comprising the step of culturing, in a medium containing a benzoylalkanoic acid, a microorganism capable of synthesizing a polyhydroxyalkanoate having a monomer unit composition represented by the following Formula [1]:AxB(1?x)??[1]from the benzoylalkanoic acid by utilizing the benzoylalkanoic acid,wherein A is at least one monomer unit represented by the following Chemical Formula [2]: ?where n is any integer of 1 to 8; B is at least one selected from monomer units represented by the following Chemical Formula [3]: ?where p is any integer of 0 to 10, and monomer units represented by the following Chemical Formula [4]: ?where q is 3 or 5; and x is not less than 0.01 to less than 1. 11. The method according to claim 10, wherein the beazoylalkanoic acid is a benzoylalkanoic acid represented by the following Chemical Formula [10]: wherein n is any integer of 1 to 8, andthe polyhydroxyalkanoate is a polyhydroxyalkanoate comprising a monomer unit represented by the following Chemical Formula [11]: ?where m is at least one or more selected from the group consisting of n, n?2, n?4 and n?6, and is an integer not less than 1. 12. The method according to claim 10, wherein the step of culturing the microorganism is a step of culturing the microorganism capable of producing the polyhydroxyalkanoate having the monomer unit composition represented by the Formula [1] in a medium containing the benzoylalkanoic acid and at least one of saccharides, by utilizing the benzoylalkanoic acid.13. The method according to claim 12, wherein the culture of the microorganism is performed in one step by using a medium containing the benzoylalkanoic acid and the saccharide.14. The method according to claim 12, wherein the culture of the microorganism is performed in at least two steps by culturing in a medium containing the benzoylalkanoic acid and the saccharide and then culturing in a medium containing the benzoylalkanoic acid and the saccharide in which a nitrogen source is limited.15. The method according to claim 12, wherein the saccharide is at least one selected from a group consisting of glucose, fructose and mannose.16. The method according to claim 10, wherein the microoganism is a microorganism belonging to Pseudomonas sp.17. The method according to claim 16, wherein the microorganism is at least one strain selected from a group consisting of Pseudomonas cichorii H45, FERM BP-7374; Pseudomonas cichorii YN2, FERM BP-7375; and Pseudomonas jessenii P161, FERM BP-7376.18. The method according to claim 10, wherein the step of culturing the microorganism is a step of culturing a microorganism capable of producing a polyhydroxyalkanoate comprising a monomer unit represented by the following Chemical Formula [5]: in a medium containing 4-benzoylbutyric acid represented by the following Chemical Formula [12]: by utilizing 4-benzoylbutyric acid; andwherein the produced polyhydroxyalkanoate comprises a monomer unit represented by the Chemical Formula [5]. 19. The method according to claim 10, wherein the step of culturing the microorganism is a step of culturing a microorganism capable of producing a polyhydroxyalkanoate comprising a monomer unit represented by the following Chemical Formula [6]: in a medium containing 5-benzoylvaleric acid represented by the following Chemical Formula [13]: by utilizing 5-benzoylvaleric acid, andwherein the produced polyhydroxyalkanoate comprises a monomer unit represented by the Chemical Formula [6]. 20. The method according to claim 10, wherein the step of culturing the microorganism is a step of culturing a microorganism capable of producing a polyhydroxyalkanoate comprising a monomer unit represented by the following Chemical Formula [7]: in a medium containing 6-benzoylhexanoic acid represented by the following Chemical Formula [14]: by utilizing 6-benzoylhexanoic acid, andwherein the produced polyhydroxyalkanoate comprises a monomer unit represented by the Chemical Formula [7]. 21. The method according to claim 10, wherein the step of culturing the microorganism is a step of culturing a microorganism capable of producing a polyhydroxyalkanoate comprising a monomer unit represented by the following Chemical Formula [8]: in a medium containing 7-benzoylheptanoic acid represented by the following Chemical Formula [15]: by utilizing 7-benzoylheptanoic acid, andwherein the produced polyhydroxyalkanoate comprises a monomer unit represented by the Chemical Formula [8]. 22. The method according to claim 10, wherein the step of culturing the microorganism is a step of culturing a microorganism capable of producing a polyhydroxyalkanoate comprising a monomer unit represented by the following Chemical Formula [9]: in a medium containing 8-benzoyloctanoic acid represented by the following Chemical Formula [16]: by utilizing 8-benzoyloctanoic acid, andwherein the produced polyhydroxyalkanoate comprises the monomer unit represented by the following Chemical Formula [9]. 23. The method according to claim 10, wherein the step of culturing the microorganism is a step of culturing a microorganism capable of producing a polyhydroxyalkanoate comprising monomer units represented by the following Chemical Formula [6]: and the following Chemical Formula [8]: in a medium containing 7-benzoylheptanoic acid represented by the following Chemical Formula [15]: by utilizing 7-benzoylheptanoic acid, andwherein the produced polyhydroxyalkanoate comprises monomer units represented by the Chemical Formulas [6] and [8]. 24. The method according to claim 10, wherein the step of culturing the microorganism is a step of culturing a microorganism capable of producing a polyhydroxyalkanoate comprising monomer units represented by the following Chemical Formula [7]: and the following Chemical Formula [9]: in a medium containing 8-benzoyloctanoic acid represented by the following Chemical Formula [16]: by utilizing 8-benzoyloctanoic acid, andwherein the produced polyhydroxyalkanoate comprises monomer units represented by the Chemical Formulas [7] and [9].
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