초록

A polyhydroxyalkanoate that comprises a unit represented by the following chemical formula (1): wherein x is an integer of 1 to 8 being the same or different in the polyhydroxyalkanoate. The microbial production process is also disclosed.

대표청구항 ▼

1. A polyhydroxyalkanoate comprising a unit represented by the following chemical formula (1): wherein x is an integer of 1 to 8 being the same or different each other in the polyhydroxyalkanoate.2. The polyhydroxyalkanoate according to claim 1, further comprising one or more units selected from 3-h

1. A polyhydroxyalkanoate comprising a unit represented by the following chemical formula (1): wherein x is an integer of 1 to 8 being the same or different each other in the polyhydroxyalkanoate.2. The polyhydroxyalkanoate according to claim 1, further comprising one or more units selected from 3-hydroxyalkanoates and 3-hydroxyalkenoates, the 3-hydroxyalkanoates being represented by the following chemical formula (2): wherein y represents an integer of 0 to 8 being the same or different each other in the polyhydroxyalkanoate; and3-hydroxyalkenoates being represented by the following chemical formula (3): wherein z is an integer of 3 or 5 being the same or different in the polyhydroxyalkanoate.3. The polyhydroxyalkanoate according to claim 1, wherein the polyhydroxyalkanoate has a number average molecular weight of 5,000 to 300,000.4. The polyhydroxyalkanoate according to claim 1, comprising a 3-hydroxy-5-[4-(methylsulfanyl)phenoxy]valerate unit represented by the following chemical formula (4): 5. A process for producing a polyhydroxyalkanoate comprising the step of culturing a microorganism in a culture medium containing a compound represented by the following chemical formula (5): wherein X is an integer of 1 to 8;wherein the polyhydroxyalkanoate comprises a unit represented by the following chemical formula (1): wherein x is an integer of 1 to 8, being the same or different each other in the polyhydroxyalkanoate.6. The process according to claim 5, wherein the polyhydroxyalkanoate further comprises one or more units selected from the group consisting of 3-hydroxyalkanoates and 3-hydroxyalkenoates represented by the following chemical formulae (2) and (3): wherein y is an integer of 0 to 8 being the same or different each other in the polyhydroxyalkanoate, wherein z is an integer of 3 or 5, being the same or different in the polyhydroxyalkanoate.7. The process according to claim 5, wherein the culture medium contains polypeptone.8. The process according to claim 5, wherein the culture medium contains yeast extract.9. The process according to claim 5, wherein the culture medium contains a saccharide.10. The process according to claim 9, wherein the culture medium contains one or more saccharides selected from the group consisting of glyceraldehyde, erythrose, arabinose, xylose, glucose, galactose, mannose, fructose, glycerol, erythritol, xylitol, gluconic acid, glucuronic acid, galacturonic acid, maltose, sucrose, and lactose.11. The process according to claim 5, wherein the culture medium contains an organic acid or a salt thereof.12. The process according to claim 11, wherein the culture medium contains one or more organic acids or a salt thereof selected from the group consisting of pyruvic acid, malic acid, lactic acid, citric acid, succinic acid, and salts thereof.13. The process according to claim 5, wherein the culture medium contains an amino acid or a salt thereof.14. The process according to claim 13, wherein the culture medium contains one or more amino acid or salts thereof selected from the group consisting of glutamic acid, aspartic acid and salts thereof.15. The process according to claim 5, wherein the culture medium contains a straight chain alkanoic acid of 4 to 12 carbons or a salt thereof.16. The process according to claim 5, wherein the cultivation step comprises two or more steps.17. The process according to claim 16, wherein the culture medium in the steps later than the first step contains no nitrogen source.18. The process according to claim 16, wherein the cultivation step comprises the steps of:(1-1) cultivating the microorganism in a culture medium that contains polypeptone and at least one compound represented by the chemical formula (5); and (1-2) further cultivating the microorganism from the step 1-1 in a culture medium containing the compound represented by the chemical formula (5) and an organic acid or a salt thereof. 19. The process according to claim 18, wherein the organic acid or a salt thereof is one or more compounds selected from the group consisting of pyruvic acid, malic acid, lactic acid, citric acid, succinic acid and salts thereof.20. The process according to claim 16, wherein the cultivation step comprises the steps of:(1-3) cultivating the microorganism in a culture medium that contains a saccharide and at least one compound represented by the chemical formula (5); and (1-4) further cultivating the microorganism from the step 1-3 in a culture medium containing the compound represented by the chemical formula (5) and a saccharide. 21. The process according to claim 20, wherein the saccharide is one or more compounds selected from the group consisting of glyceraldehyde, erythrose, arabinose, xylose, glucose, galactose, mannose, fructose, glycerol, erythritol, xylitol, gluconic acid, glucuronic acid, galacturonic acid, maltose, sucrose, and lactose.22. The process according to claim 5, wherein the compound represented by the chemical formula (5) is 5-[4-(methylsulfanyl)phenoxy]valeric acid represented by the following chemical formula (6): and the unit is represented by the following chemical formula (4): 23. The process according to claim 5, further comprising the step of isolating the polyhydroxyalkanoate from cells of the microorganism cultivated in the cultivation step.24. The process according to claim 23, wherein the step of isolating the polyhydroxyalkanoate comprises the step of treating with a solvent to solubilize and extract the polyhydroxyalkanoate accumulated in the cells of the microorganism cultivated in the cultivation step.25. The process according to claim 24, wherein the solvent is one or more solvents selected from the group consisting of chloroform, dichloromethane, dioxane, tetrahydrofuran, acetonitrile, and acetone.26. The process according to claim 23, wherein the step of isolating the polyhydroxyalkanoate comprises the step of disrupting cells of the microorganism.27. The process according to claim 26, wherein the cells are disrupted by ultrasonic disruption, homogenization, pressure disruption, disruption with glass beads, trituration, grinding or freeze-thawing.28. The process according to claim 5, wherein the microorganism belongs to genus Pseudomonas. 29. The process according to claim 28, wherein the microorganism that belongs to genus Pseudomonas is selected from the group consisting of Pseudomonas cichorii YN2 (FERM BP-7375), Pseudomonas cichorii H45 (FERM PB-7374), and Pseudomonas jessenii P161 (FERM BP-7376).