[특허]Magnetic pigment

Magnetic pigment 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12N-015/00 C12N-013/00 C12P-019/34 C07H-021/00
출원번호	US-0756743 (2001-01-10)
우선권정보	DE-0020398 (1995-06-08); DE-0037985 (1995-10-12)
발명자 / 주소	Kleiber, J?rg Walter, Thomas Harttig, Herbert Lesniak, Christoph Mennig, Martin Riedling, Michael Schmidt, Helmut
출원인 / 주소	Roche Diagnostics GmbH
인용정보	피인용 횟수 : 12 인용 특허 : 91

초록 ▼

Magnetic particles are prepared containing a magnetic core coated with a glass layer having a substantially pore-free class surface or having pores with a diameter offices than 10 nm. The particles are used for separating biological material such as nucleic acids. A preferred process of preparing the particles is by forming a mixture of magnetic cores with a sol formed from an alcohol and a metal alkoxide, spray-drying the mixture to coat the cores with a layer of gelled sol, and heating the coated cores to obtain the magnetic glass particles. Preferably, the particles have an average particle size of less than 100 μm. The magnetic core may be a composite material containing a mica core and magnetite particles immobilized on the mica core, and the glass layer may contain boron oxide. Magnetic core materials include magnetite (Fe3O4) and Fe2O3.

대표청구항 ▼

1. A method for separating nucleic acids from a fluid containing the nucleic acids comprising:a) bringing a sample that contains nucleic acids in a fluid in contact with a magnetic particle comprising a magnetic core and an outer glass layer, wherein said outer glass layer comprises boron oxide, said outer glass layer completely covers the magnetic core and has an outer glass surface, wherein the outer glass surface is substantially pore-free or has pores with a diameter of less than 10 nm under conditions such that the nucleic acids can bind to the glass surface; and thereafter b) separating the bound nucleic acids from the fluid. 2. The method of claim 1 wherein said nucleic acids in the fluid are nucleic acids in native form.3. The method of claim 1 wherein the bound nucleic acids are separated from the fluid with a magnetic field.4. The method of claim 1 wherein the magnetic particle is not subjected to the influence of a magnetic field before being brought in contact with the sample and is thus able to sediment when being brought in contact with the sample.5. The method of claim 1 wherein any pores in the glass surface of said magnetic particle have a diameter of less than 1 nm.6. The method of claim 1, further comprising eluting said bound nucleic acids from said magnetic particle.

이 특허에 인용된 특허 (91)

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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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