The present invention discloses novel pest control compounds comprising NPF polypeptides and methods for using such compounds in the control of pests.
대표청구항▼
1. A method of controlling a pest wherein said method comprises applying to the pest, or to a pest-inhabited locus, transformed cells expressing an exogenous polynucleotide encoding a pesticidal polypeptide, wherein the pesticidal polypeptide comprises an amino acid sequence selected from the group
1. A method of controlling a pest wherein said method comprises applying to the pest, or to a pest-inhabited locus, transformed cells expressing an exogenous polynucleotide encoding a pesticidal polypeptide, wherein the pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO;14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24, and wherein the pest ingests the pesticidal polypeptide.2. The method of claim 1, wherein the pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:3.3. The method of claim 1, wherein the pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO. 4.4. The method of claim 1, wherein the pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24.5. The method of claim 1, wherein the pesticidal polypeptide is a fusion polypeptide.6. The method of claim 5, wherein the fusion polypeptide is a multimer or the amino acid sequence.7. The method of claim 1, wherein the cells are pest food cells comprising the polynucleotide encoding the pesticidal polypeptide.8. The method of claim 1, wherein the cells are algae cells.9. The method of claim 1, wherein the cells are a Clorella species.10. The method of claim 1, wherein the cells are yeast cells.11. The method of claim 1, wherein the cells are applied in a living state.12. The method of claim 1, wherein the cells are applied in a non-living state.13. The method of claim 1, wherein the cells are applied as a component of a pesticidal composition which also comprises a pesticidally acceptable carrier.14. The method of claim 1, wherein the pest utilizes a serine esterase as a digestive enzyme, and wherein the pesticidal polypeptide inhibits synthesis of the serine esterase within the pest.15. The method of claim 1, wherein the pest utilizes trypsin as a digestive enzyme, and wherein the pesticidal polypeptide inhibits synthesis of the trypsin within the pest.16. The method of claim 1, wherein the pest is selected from the group consisting of coleopterans, lepidopterans, and dipterans.17. The method of claim 1, wherein the pest is a blood-sucking pest.18. The method of claim 1, wherein the pest is of the suborder Nematocera.19. The method of claim 1, wherein the pest is a pest of the family Colicidae.20. The method of claim 1, wherein the pest is a dipteran.21. The method of claim 1, wherein the pest is of a genus selected from the group consisting of Heliothis, Culex, Theobaldia, Aedes, Anopheles, Forciponiyia, Culicoides and Helea. 22. The method of claim 1, wherein the pest is selected from the group consisting of mosquitoes, flesh flies, fleas, sand flies, house flies, and dog flies.23. The method of claim 1, wherein the pest is a mosquito.24. The method of claim 1, wherein the pest is of a species selected from the group consisting of: Aedes aegypti, Culex quinquefasciatus, Anopheles albimanus, Anopheles quadrimaculatus, Lutzomyia anthrophora, Culicoides variipennis, Stomoxys calcitrans, Musca domestica, Ctenocephalides felis, and Heliothis virescens. 25. The method of claim 1, wherein said method comprises applying the cells to a pest-inhabited locus.26. The method of claim 25, wherein the pest-inhabited locus is a body of water.27. The method of claim 1, wherein the cells are applied in association with a pest food.28. A method for preparing a pesticidal composition comprising transforming a host cell with an exogenous polynucleotide encoding a pesticidal polypeptide, operatively linked to a promoter, and bringing the transformed host cell into association with a pesticidally acceptable carrier, wherein the pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24.29. The method of claim 28, wherein the pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:3.30. The method of claim 28, wherein the pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:4.31. The method of claim 28, wherein the pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24.32. The method of claim 28, wherein the pesticidal polypeptide is a fusion polypeptide.33. The method of claim 32, wherein the fusion polypeptide is a multimer of the amino acid sequence.34. An expression vector comprising a promoter and a polynucleotide encoding a pesticidal polypeptide wherein the promoter has the capacity to control expression of said polynucleotide in a host cell, and wherein said pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24.35. The expression vector of claim 34, wherein said pesticidal polypeptide comprises the amino acid of SEQ ID NO:3.36. The expression vector of claim 34, wherein said pesticidal polypeptide comprises the amino acid of SEQ ID NO:4.37. The expression vector of claim 34, wherein said pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24.38. The expression vector of claim 34, wherein said pesticidal polypeptide is a fusion polypeptide.39. The expression vector of claim 38, wherein said fusion polypeptide is a multimer of said amino acid sequence.40. A transformed cell comprising an exogenous polynucleotide encoding a pesticidal polypeptide, operatively linked to a promoter, wherein said cell expresses said polynucleotide to produce said pesticidal polypeptide, and wherein said pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24.41. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:3.42. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:4.43. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:6.44. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:8.45. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:10.46. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:12.47. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:14.48. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:16.49. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:18.50. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:20.51. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:22.52. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:23.53. The transformed cell of claim 40, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:24.54. The transformed cell of claim 40, wherein the transformed cell is a pest food.55. The transformed cell of claim 40, wherein the transformed cell is mosquito larvae food.56. The transformed cell of claim 40, wherein the transformed cell is an algae cell.57. The transformed cell of claim 40, wherein the transformed cell is a green algae cell.58. The transformed cell of claim 40, wherein the transformed cell is a Clorella species.59. The transformed cell of claim 40, wherein the transformed cell is a yeast cell.60. A pesticidal composition comprising: transformed cells expressing an exogenous polynucleotide encoding a pesticidal polypeptide, operatively linked to a promoter, wherein said pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24; and a pesticidally acceptable carrier.61. The pesticidal composition of claim 60, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:3.62. The pesticidal composition of claim 60, wherein said pesticidal polypeptide comprises the amino acid sequence of SEQ ID NO:4.63. The pesticidal composition of claim 60, wherein said pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24.64. The pesticidal composition of claim 60, wherein said composition is in a form selected from the group consisting of pellets, briquettes, bricks, powders, granules, sprays, solutions and capsules.65. The pesticidal composition of claim 60, wherein said composition is formulated to float on an aqueous medium.66. The pesticidal composition of claim 60, wherein said composition is formulated to maintain a depth of 0 to 2 feet below the surface of an aqueous medium.67. The pesticidal composition of claim 60, wherein said composition is formulated to sink in an aqueous medium.68. An expression vector comprising a promoter and a polynucleotide encoding a pesticidal polypeptide wherein the promoter has the capacity to control expression of said polynucleotide in a host cell, and wherein the pesticidal polypeptide consists of an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24.69. A transformed cell comprising an exogenous polynucleotide encoding a pesticidal polypeptide, operatively linked to a promoter, wherein said cell expresses said polynucleotide to produce said pesticidal polypeptide, and wherein said pesticidal polypeptide consists of an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:23, and SEQ ID NO:24.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (48)
Hall Frederick L. ; Nimni Marcel E. ; Tuan Tai-Lan ; Wu Lingtau ; Cheung David T., Artificial skin prepared from coclagen matrix containing transforming growth factor-.beta. having a collagen binding si.
Hatton Leslie Roy,GB2 ; Buntain Ian George,GB2 ; Hawkins David William,GB2 ; Parnell Edgar William,GB2 ; Pearson Christopher John,GB2 ; Roberts David Alan,GB2, Derivatives of N-phenylpyrazoles.
Johnson Janice H. (Salt Lake City UT) Kral ; Jr. Robert M. (Salt Lake City UT) Krapcho Karen (Salt Lake City UT), Insecticidal peptides from spider venom.
Krapcho Karen Joanne ; Jackson John Randolph Hunter ; Johnson Janice Helen ; DelMar Eric George ; Kral ; Jr. Robert Marden, Insecticidally effective peptides.
Nacht Sergio ; Won Richard ; Katz Martin A. ; Cheng Tai ; Liau Christine J. Y. ; Eury Robert P. ; Froix Michael, Ionic beads for controlled release and adsorption.
Boden Christopher D. J. (Nottingham GB3) Chambers John (Maidenhead GB3) McGreevy Paul B. (Slough GB3) Dendy Julie A. (Slough GB3) Stevens Ian (Southampton GB3), Lactone insect lures.
Demarest Scott W. (Racine WI) Mayer William J. (South Barrington IL) Gruber Dennis W. (Arlington Heights IL) McCumber Donald E. (Madison WI) Reimer Joseph E. (Oregon WI) Kohler Karl A. (Racine WI), Method and apparatus for catching insects.
Fuchs Roy L. (St. Charles MO) Kishore Ganesh M. (Chesterfield MO) MacIntosh Susan C. (St. Louis MO), Method for improving the efficacy of insect toxins.
Schulte Peter (Alzenau-Hoerstein DEX) Volkmer Michael (Hanau DEX) Kuhn Frank (Gelhausen DEX), Method for the degradation of harmful substances in water by means of hydrogen peroxide under UV irradiation.
Duffy Eric P. (13561 Rawhide Pkwy. Farmers Branch TX 75234) McDaniel ; Jr. Joe D. (1112 Alameda Carrollton TX 75007) Donahue William A. (2307 Harbinger La. Dallas TX 75287), Pesticidal microemulsion formulation.
Kingan Timothy G. (Catonsville MD) Raina Ashok K. (Beltsville MD), Pheromonostatic peptide used to disrupt mating of the corn earworm, helicoverpa zea.
Bok Song H. (Daejeon KRX) Lee Hang W. (Daejeon KRX) Son Kwang H. (Daejeon KRX) Kim Sung U. (Daejeon KRX) Lee Jee W. (Daejeon KRX) Kim Do Y. (Daejeon KRX) Kwon Yong K. (Daejeon KRX), Process for preparing coated microbial pesticides and pesticides produced therefrom.
Vickell Gregg A. (Burlington CAX) Norcross Roy (Norwalk CT) Chattopadhyay Jaganmay (Paramus NJ), Process for the detoxification of effluents containing free or complexed cyanides.
Hilder Vaughan A. (Durham GB3) Gatehouse Angharad M. R. (Durham GB3) Powell Kevin (Durham GB3) Boulter Donald (Durham City GB3), Proteins with insecticidal properties against homopteran insects and their use in plant protection.
Levy Richard (Fort Myers FL), Terrestrial delivery compositions and methods for controlling insect and habitat-associated pest populations in terrestr.
Bogaert, Thierry AndréOlivier Eddy; Zwaal, Richard; Plaetinck, Geert Karel Maria; De Kerpel, Jan Octaaf; Kaletta, Titus Jan, Method and constructs for delivering double stranded RNA to pest organisms.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.