[특허]Magnetic separation apparatus and methods

[미국특허] Magnetic separation apparatus and methods 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	B01D-035/06 G01N-033/53
출원번호	US-0733829 (2003-12-10)
발명자 / 주소	Terstappen, Leon W. M. M. Dolan, Gerald
출원인 / 주소	Immunivest Corporation
대리인 / 주소	Immunicon Corp
인용정보	피인용 횟수 : 36 인용 특허 : 19

초록 ▼

Apparatuses and methods for separating, immobilizing, and quantifying biological substances from within a fluid medium. Biological substances are observed by employing a vessel (6) having a chamber therein, the vessel comprising a transparent collection wall (5). A high internal gradient magnetic capture structure may be on the transparent collection wall (5), magnets (3) create an externally-applied force for transporting magnetically responsive material toward the transparent collection wall (5). The magnetic capture structure comprises a plurality of ferromagnetic members and has a uniform or non-uniform spacing between adjacent members. There may be electrical conductor means supported on the transparent collection wall (5) for enabling electrical manipulation of the biological substances. The chamber has one compartment or a plurality of compartments with differing heights. The chamber may include a porous wall. The invention is also useful in conducting quantitative analysis and sample preparation in conjunction with automated cell enumeration techniques.

대표청구항 ▼

1. An apparatus for observing magnetically responsive microscopic entities suspended in a fluid member, comprising:a. a vessel having a transparent wall and a chamber formed therein for containing the fluid medium; b. a ferromagnetic capture structure supported on the interior surface of the transparent wall; c. magnetic means for inducing an internal magnetic gradient in the vicinity of the ferromagnetic capture structure, whereby the magnetically responsive entities are immobilized along the wall adjacent to the capture structure; and d. electrical conductor means supported on the transparent wall for enabling electrical manipulation of the immobilized entities.

이 특허에 인용된 특허 (19) 인용/피인용 타임라인 분석

Liberti Paul A. (Churchville) Feeley Brian P. (Easton) Gohel Dhanesh I. (Philadelphia PA), Apparatus and methods for magnetic separation.
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Liberti Paul A. (Churchville PA) Wang Yuzhou (Wayne PA) Tang Weixin (Devon PA) Feeley Brian P. (Easton PA) Gohel Dhanesh I. (Philadelphia PA), Apparatus and methods for magnetic separation featuring external magnetic means.
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Garaschenko Vyacheslav I. (Rovno SUX) Sandulyak Alexandr V. (Rovno SUX) Volkov Igor V. (Rovno SUX), Apparatus for separation of ferromagnetic materials from fluid media.
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Slezak Sue E. (Downingtown PA) Gray Brian D. (Ardmore PA) Kopia Gregory A. (Phoenixville PA), Bio-analytical separation method.
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Fujiwara Koichi (Mito JPX) Mizutani ; deceased Hiromichi (late of Tokyo JPX by Hiroko Mizutani ; legal representative), Device for collecting or preparing specimens using magnetic micro-particles.
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Kindler Andrew (San Marino CA), Electrochemical immunosensor system and methods.
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Dolan Gerald J. ; Terstappen Leon W. M. M., Magnetic devices and sample chambers for examination and manipulation of cells.
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Zborowski Maciej (Lakewood OH) Malchesky Paul S. (Painesville OH), Magnetic field separation and analysis system.
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Dolan Gerald J. ; Terstappen Leon W. M. M., Magnetic separation apparatus and methods employing an internal magnetic capture gradient and an external transport forc.
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Groner Warren (Whitestone NY), Method and apparatus for determining the count per unit volume of particles.
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Tycko Daniel H. (Stony Brook NY), Method and apparatus for determining the volume & index of refraction of particles.
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Shenkin Mark L. (Pembroke Pines FL) Hamelik Ronald M. (Miami FL) Wood James C. S. (Coral Springs FL), Method and materials for determining particle count in a flow cytometer.
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Fujiwara Koichi (Mito JPX) Mizutani ; deceased Hiromichi (late of Tokyo JPX by Hiroko Mizutani ; Tokyo ; legal representative), Method for collecting and preparing specimens for immune reactions.
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Sinor Lyle T. (Roswell GA) Eatz Ralph A. (Roswell GA), Method for drying mammalian cells for use in solid phase immunoassays and articles incorporating same.
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Wang Yuzhou (Wayne PA) Tang Weixin (Landsdale PA) Cronin William J. (Matawan NJ) Liberti Paul A. (Huntingdon Valley PA), Method for magnetic separation featuring magnetic particles in a multi-phase system.
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Miltenyi Stefan (Moitzfeld 60a D-5060 Bergisch Gladbach 1 ; Cologne DEX), Methods and materials for improved high gradient magnetic separation of biological materials.
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Liberti Paul A. ; Wang Yuzhou, Methods for magnetic immobilization and manipulation of cells.
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Lea Tor E. (Oslo NOX) Pedersen Bjorn K. (Haslum NOX) Naess Harald K. (Oslo NOX), Process and apparatus for counting particles.
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Weinreb Arye (Jerusalem ILX) Deutsch Mordechai (Petah-Tikvah ILX), System and methods for cell selection.
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이 특허를 인용한 특허 (36) 인용/피인용 타임라인 분석

Dryga, Sergey A.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Esch, Victor C., Analyzing bacteria without culturing.
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Dryga, Sergey A.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Esch, Victor C., Analyzing bacteria without culturing.
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Davis, Ronald W.; Jeffrey, Stefanie S.; Mindrinos, Michael N.; Pease, R. Fabian; Powell, Ashley Ann; Talasaz, AmirAli Hajhossein, Apparatus for magnetic separation of cells.
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Halbert, David D.; Kuslich, Christine; Poste, George; Klass, Michael; Spetzler, David; Pawlowski, Traci; Tasinato, Andrea; Holterman, Daniel, Biomarkers for theranostics.
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Chang, Ying-Chih; Wu, Han-Chung; Tseng, Po-Yuan; Wu, Jen-Chia, Capture, purification, and release of biological substances using a surface coating.
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Kuslich, Christine; Poste, George; Klass, Michael; Spetzler, David; Pawlowski, Traci, Circulating biomarkers for metastatic prostate cancer.
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Shao, Hung-Jen, Collection of suspended cells using a transferable membrane.
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Chang, Ying-Chih; Lai, Jr-Ming; Wu, Jen-Chia, Collector architecture layout design.
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Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Compositions for isolating a target analyte from a heterogeneous sample.
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Yu, Liping; Warner, Brian David; Trotter, Joseph T., Devices and methods for manipulating components in a fluid sample.
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Yu, Liping; Warner, Brian David; Trotter, Joseph T., Devices and methods for manipulating components in a fluid sample.
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Yu, Liping; Warner, Brian David; Trotter, Joseph T., Devices and methods for manipulating components in a fluid sample.
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Yu, Liping, Devices and methods for separating magnetically labeled moieties in a sample.
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Esch, Victor C.; Dryga, Sergey A.; Clarizia, Lisa-Jo Ann, Extracting low concentrations of bacteria from a sample.
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Scholtens, Tyco M.; Schreuder, Frederik; Greve, Jan; Tibbe, Arjan G. J.; Terstappen, Leon W. M. M., Imaging of immunomagnetically enriched rare cells.
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Scholtens, Tycho M.; Terstappen, Leon L. M. M.; Tibbe, Arjan G. J., Magnetic separation apparatus and methods.
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Wang, Shan X.; Pourmand, Nader; White, Robert L., Magnetic sifter.
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Coumans, Frank A. W.; Greve, Jan; Modica, Frank P.; Terstappen, Leon W. M. M.; Tibbe, Arjan G. J.; Verrant, John A., Methods and algorithms for cell enumeration in a low-cost cytometer.
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Coumans, Frank A. W.; Greve, Jan; Modica, Frank P.; Terstappen, Leon W. M. M.; Tibbe, Arjan G. J.; Verrant, John A., Methods and algorithms for cell enumeration in a low-cost cytometer.
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Droog, Erik; Gohel, Dhanesh; Tibbe, Arjan G. J.; Greve, Jan; Terstappen, Leon W. M. M., Methods and algorithms for cell enumeration in a low-cost cytometer.
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Dryga, Sergey A., Methods for degrading nucleic acid.
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Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Methods for isolating a target analyte from a heterogeneous sample.
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Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Methods for isolating a target analyte from a heterogeneous sample.
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Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Methods for isolating a target analyte from a heterogeneous sample.
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Dryga, Sergey A.; Esch, Victor C.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Ung, Thearith H.; Sitdikov, Ravil A., Methods for isolating a target analyte from a heterogenous sample.
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Scholl, David R.; Brown, James L.; Jollick, Jr., Joseph A.; Lollar, Ronald, Methods for liquid direct fluorescent antibody intracellular virus detection.
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Dykes, Colin; Dryga, Sergey A.; Clarizia, Lisa-Jo Ann; Adams, Eddie W.; Norvell, Meghan, Methods for raising antibodies.
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Dykes, Colin, Methods for universal target capture.
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Achrol, Achal Singh; Sethu, Palaniappan, Microfluidic method and system for isolating particles from biological fluid.
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Adolphi, Natalie Louise; McDowell, Andrew Frederick, NMR, instrumentation, and flow meter/controller continuously detecting MR signals, from continuously flowing sample material.
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Moll, Kevin D.; Delaney, Marie J.; Vogel, Kurt R.; Lochhead, Michael J.; Ives, Jeffrey; Dunn, John S.; Rowley, Keagan B., Particle identification system, cartridge and associated methods.
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Macemon, James H., Sample entry.
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Dryga, Sergey A.; Esch, Victor C.; Saul, Richard G.; McDowell, Andrew F., Separating target analytes using alternating magnetic fields.
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Dunn, John S.; Ives, Jeffrey T.; Lochhead, Michael J.; Vogel, Kurt R.; Rowley, Keagan B., System and device for analyzing a fluidic sample.
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Sitdikov, Ravil A.; Adams, Eddie W.; Torrance, Magdalena A.; Aley, David K.; Smith, Erik J.; Esch, Victor C., Target capture system.
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Norvell, Meghan, Target detection.
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[미국특허] Magnetic separation apparatus and methods 원문보기