초록 ▼

Endothelial protein C receptor (EPCR) is found primarily on endothelial cells of large vessels. EPCR translocates from the plasma membrane surface to the nucleus. Molecules which bind to EPCR can be carried from the plasma membrane surface to the nucleus. These molecules include antibodies to EPCR a

Endothelial protein C receptor (EPCR) is found primarily on endothelial cells of large vessels. EPCR translocates from the plasma membrane surface to the nucleus. Molecules which bind to EPCR can be carried from the plasma membrane surface to the nucleus. These molecules include antibodies to EPCR and activated protein C. Protein C, which also binds to EPCR, can be internalized by endothelial cells, but does not enter the nucleus. Thus, EPCR translocation from the plasma membrane to the nucleus provides a means of delivering nucleic acid such as DNA, proteins such as transcription factors, diagnostic agents or other types of drugs to the nucleus of endothelial cells, particularly those on large blood vessels. Conjugates of the materials to be delivered to the nucleus can be formed by ionic or covalent coupling. For example, proteins, including fusion proteins, can be directly conjugated to an anti-EPCR monoclonal antibody. Covalent attachment of positively charged polymers, such as polylysine, to an anti-EPCR antibody allows nucleic acid to bind by ionic charges. Steptavidin and biotin can also be used to conjugate molecules to anti-EPCR antibodies. These conjugated antibodies are transported to the nucleus by EPCR. Eamples demonstrate selective transport to the nucleus which is mediated by EPCR. Molecules transported include activated protein C, antibodies to EPCR, and steptavidin-biotin conjugates. Modification of anti-EPCR monoclonal antibodies by covalently coupling to polylysine allows binding of an expression vector to the modified antibody and translocation to the nucleus.

대표청구항 ▼

1. A method for selectively delivering a molecule to the nucleus of endothelial cells of large vessels, comprisingdirectly administering a conjugate to large vessel endothelial cells,wherein the conjugate comprises an agent binding selectively to endothelial protein C receptor (EPCR) which causes up

1. A method for selectively delivering a molecule to the nucleus of endothelial cells of large vessels, comprisingdirectly administering a conjugate to large vessel endothelial cells,wherein the conjugate comprises an agent binding selectively to endothelial protein C receptor (EPCR) which causes uptake by the cell and transfer into the nucleus of the molecule to be delivered,wherein the conjugate is formed between the molecule to be delivered and an agent selected from the group consisting of activated protein C and an antibody to EPCR.2. The method of claim 1 wherein the conjugate is formed between the molecule to be delivered and an antibody to EPCR.3. The method of claim 1 wherein the conjugate is formed between the molecule to be delivered and activated protein C.4. The method of claim 1 wherein the conjugate comprises a chimeric antibody binding to the molecule to be delivered and to EPCR.5. The method of claim 1 wherein the molecule to be delivered is a nucleic acid molecule, wherein the nucleic acid molecule is a gene or cDNA under the control of a promoter expressed in the nucleus of an endothelial cell, wherein the nucleic acid molecule is delivered by directly contacting the endothelial cells of large vessels with the nucleic acid molecule conjugate or by catheterization to the endothelial cells.6. The method of claim 5 wherein the nucleic acid molecule is selected from the group consisting of triplex forming oligonucleotides, ribozymes, guide sequences for ribozymes, and antisense.7. The method of claim 1 wherein the molecule to be delivered is selected from the group consisting of drugs, proteins and diagnostic agents.8. The method of claim 1 wherein the molecule to be delivered is a protein.9. The method of claim 8 wherein the protein is a transcription factor.10. The method of claim 1 wherein the molecule to be delivered is coupled to the agent which binds to EPCR by molecules selected from the group consisting of streptavidin and biotin, and molecules having multiple positive charges.11. The method of claim 1 wherein the conjugate is administered to large vessel endothelial cells in culture or isolated from an individual.12. The method of claim 1 wherein the conjugate is administered directly to an individual.13. A conjugate comprising an agent binding selectively to endothelial protein C receptor (EPCR) selected from the group consisting of activated protein C and antibodies reactive with EPCR, and a nucleic acid molecule to be delivered to a large vessel endothelial cell, wherein the conjugate is a chemical conjugate, or conjugate formed by indirect binding by a positively charged polymer, chimeric antibody or streptavidin.14. The conjugate of claim 13 wherein the conjugate is formed between the nucleic acid molecule to be delivered and activated protein C.15. The conjugate of claim 13 wherein the molecule to be delivered is a nucleic acid molecule in combination for means for directly contacting the nucleic acid molecule conjugate directly with the endothelial cells of large vessels, wherein the means are for in vitro treatment of the cells or by catheterization to the endothelial cells.16. The conjugate of claim 15 wherein the nucleic acid molecule is a gene or cDNA under the control of a promoter expressed in the nucleus of an endothelial cell.17. The conjugate of claim 15 wherein the nucleic acid molecule is selected from the group consisting of triplex forming oligonucleotides, ribozymes, guide sequences for ribozymes, and antisense.18. The conjugate of claim 13 comprising coupling means which binds the nucleic acid molecule to be delivered to the agent which binds EPCR.19. The conjugate of claim 18 wherein the coupling means is a positively charged polymer or molecule.20. The conjugate of claim 18 wherein the coupling means is streptavidin-biotin.21. The conjugate of claim 13 comprising a chimeric antibody which binds to EPCR and to the nucleic acid molecule to be delivered.