Capillary array and electrophoresis apparatus
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-027/453
G01N-030/02
출원번호
US-0239765
(2000-12-04)
국제출원번호
PCT//JP00/02373
(2002-10-24)
§371/§102 date
20021024
(20021024)
국제공개번호
WO01//84134
(2001-11-08)
발명자
/ 주소
Morioka, Tomonari
Shimizu, Yasushi
Ugai, Seiichi
Kita, Toshiaki
Tsukada, Kiyoshi
출원인 / 주소
Hitachi, Ltd.
대리인 / 주소
Dickstein Shapiro Morin &
인용정보
피인용 횟수 :
2인용 특허 :
8
초록▼
A capillary array comprising multiple capillaries and two holding substrates for arranging the aforementioned multiple capillaries in a substantially plane surface by sandwiching at least some of these capillaries mutually. One of the holding substrates has a detection window for transmitting the de
A capillary array comprising multiple capillaries and two holding substrates for arranging the aforementioned multiple capillaries in a substantially plane surface by sandwiching at least some of these capillaries mutually. One of the holding substrates has a detection window for transmitting the detected light emitted by a test sample by irradiation of light. Especially, this detection window comprises multiple light transmission areas provided to correspond to each capillary to transmit the detected light, and light cut-off areas provided between these light transmission areas to cut off the detected light. These light cut-off areas cut off light reflected from the surface of capillaries and hence improve the SN ratio, thereby ensuring high-precision detection.
대표청구항▼
1. A capillary array unit comprising:multiple capillaries, and a window unit, wherein at least some of said capillaries are arranged in a substantially plane surface, said window unit holding said capillaries arranged in said plane surface and having a detection window for transmitting detected ligh
1. A capillary array unit comprising:multiple capillaries, and a window unit, wherein at least some of said capillaries are arranged in a substantially plane surface, said window unit holding said capillaries arranged in said plane surface and having a detection window for transmitting detected light emitted from a portion arranged in said plane surface by irradiation of light, said window unit comprising at least first and second holding substrates; wherein said at least first and second holding substrates sandwich some of said multiple capillaries to provide said substantially plane surface; and said detection window being formed on said holding substrate; said detection window further comprising: multiple light transmission areas provided to correspond to said capillaries to transmit detected light, and multiple light cut-off areas provided adjacent to said light transmission areas. 2. A capillary array unit comprising:multiple capillaries to allow migration of test samples, and a window unit further comprising first and second holding substrates for sandwiching at least some of said capillaries mutually to arrange them in a substantially plane surface, wherein a detection window for transmitting detected light emitted in the direction normal to said plane surface from said test samples is formed on said first holding substrate; said detection window formed on the first holding substrate being characterized by comprising: multiple light transmission areas provided to correspond to said capillaries to transmit said detected light, and multiple light cut-off areas provided adjacent to said light transmission area to cut off said detected light. 3. A capillary array unit according to claim 1 or 2 wherein said first holding substrate is a silicon substrate; said light transmission areas are created by multiple rectangular openings formed on said silicon substrate; and said light cut-off areas are made up of strip-shaped areas formed between these multiple openings.4. A capillary array unit according to claim 3 characterized in that each of the said strip-shaped light cut-off areas has a trapezoidal cross section cut off in the direction substantially normal to the longitudinal direction of multiple capillaries arranged in said plane surface; and the width of said cross section on the side where said first substrate is opposite to said second substrate is smaller than the width of said cross section on the side reverse to the surface opposite to said second substrate.5. A capillary array unit according to claim 1 or 2 wherein said first holding substrate is formed by a high light-transmittance member; a light cut-off film made of low a light-transmittance member is formed on the surface of one side; and said light transmission areas for transmitting detected light are formed by removing said light cut-off film in a rectangular shape corresponding to said capillaries.6. A capillary array unit according to claim 5 wherein grooves for positioning between said capillaries and said light transmission areas are provided on the side of said first holding substrate facing toward said second holding substrate of said window unit, said grooves being provided at least in said light transmission areas and extending in the longitudinal direction of the capillaries arranged in a plane surface;said grooves having a V-like configuration in cross section normal to the longitudinal direction of said capillaries. 7. A capillary array unit according to claim 1 or 2 wherein positioning grooves having a V-like configuration in cross section for arranging said multiple capillaries in a substantially plane surface and opposite to said light transmission areas are provided on the side of the first holding substrate opposite to said second holding substrate and around said detection window.8. An electrophoresis apparatus comprising:multiple capillaries to allow migration of test samples and having an area to which an excitation light is irradiated; a holding member for arranging at least some of said multiple capillaries including said area substantially in a plane; and a holding substance having multiple light transmission areas formed as openings provided on said holding substance, which are provided to correspond to said multiple capillaries arranged in said holding substance and multiple light cut-off areas provided between said multiple light transmission areas, wherein said at least some of said multiple capillaries are sandwiched between said holding member and said holding substance; the electrophoresis apparatus further comprising: a light exciting system for irradiating said excitation light to said capillaries; and a light receiving optical system for detecting a fluorescent light from the migration of said test samples. 9. An electrophoresis apparatus according to claim 8, wherein said holding substance is a silicon substrate.10. An electrophoresis apparatus according to claim 8, wherein said light transmission areas include at least one strip shaped light cut-off area and pass through the opening provided on said holding substance.11. An electrophoresis apparatus according to claim 10, wherein each said strip shaped light cut-off area has a trapezoidal cross section as seen in cross-section taken in a direction substantially normal to a longitudinal direction of said multiple capillaries arranged substantially in a plane.12. An electrophoresis apparatus according to claim 11, wherein a width of said cross section on a side of said holding substance facing toward said holding member is smaller than a width of said cross section on a side of said holding substance facing away from said holding member.13. An electrophoresis apparatus according to claim 10, wherein said light cut-off area is formed with a light cut-off film of a low light transmittance member formed on a capillary surface adjacent said holding substance.14. An electrophoresis apparatus according to claim 8, wherein said holding substance also has plural positioning grooves provided to correspond to said multiple light transmission areas.15. An electrophoresis apparatus according to claim 8, wherein a portion of said holding member is configured for reducing reflected light from the holding member.16. An electrophoresis apparatus according to claim 15, wherein said holding member is a glass substrate, and a part of said glass substrate is provided with a frosted glass surface.17. A capillary array comprising;multiple capillaries having an area to which an excitation light is irradiated; a holding member for arranging some of said capillaries including said area in a substantially plane shape; and a holding substrate having multiple light transmission areas which are provided to correspond to said multiple capillaries arranged in said holding substrate and multiple light cut-off areas provided between said multiple light transmission areas, wherein said some of said multiple capillaries are sandwiched between said holding member and said holding substrate; and wherein said light transmission area is an opening provided on said holding substrate. 18. An electrophoresis apparatus according to claim 17, wherein said holding substrate is a silicon substrate.19. An electrophoresis apparatus according to claim 17, wherein said light transmission areas include at least one strip shaped light cut-off area and pass through the opening provided on said holding substrate.20. An electrophoresis apparatus according to claim 19, wherein each said strip shaped light cut-off area has a trapezoidal cross section as seen in cross-section taken in a direction substantially normal to a longitudinal direction of said multiple capillaries arranged substantially in a plane.21. An electrophoresis apparatus according to claim 20, wherein a width of said cross section on a side of said holding substrate facing toward said holding member is smaller than a width of said cross section on a side of said holding substrate facing away from said holding member.22. An electrophoresis apparatus according to claim 19, wherein said light cut-off area is formed with a light cut-off film of a low light transmittance member formed on a capillary surface adjacent said holding substrate.23. An electrophoresis apparatus according to claim 17, wherein said holding substrate also has plural positioning grooves provided to correspond to said multiple light transmission areas.24. An electrophoresis apparatus according to claim 17, wherein a portion of said holding member is configured for reducing reflected light from said holding member.25. An electrophoresis apparatus according to claim 24, wherein said holding member is a glass substrate, and a part of said glass substrate is provided with a frosted glass surface.
Yeung Edward S. (Ames IA) Chang Huan-Tsang (Silver Spring MD) Fung Eliza N. (Ames IA) Li Qingbo (Ames IA) Lu Xiandan (Ames IA), Multiplexed capillary electrophoresis system.
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