Apparatus for purifying nucleic acids and proteins
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
B03C-001/01
B03C-001/005
B01D-035/06
B01L-003/02
출원번호
US-0235496
(2002-09-06)
우선권정보
JP-11-251826(1999-09-06)
발명자
/ 주소
Ikeda,Katsunori
Nishiya,Yoshiaki
Kusumoto,Masahiro
Suzuki,Masahiro
Kawamura,Yoshihisa
출원인 / 주소
Toyo Boseki Kabushiki Kaisha
대리인 / 주소
Kenyon &
인용정보
피인용 횟수 :
19인용 특허 :
10
초록▼
A method for purifying nucleic acids or proteins comprising a plurality of piston pumps; and a plurality of nozzles capable of having a plurality of disposable tips which are automatically attachable/detachable. The apparatus includes a mechanism for mixing a liquid mixture of each sample and a reag
A method for purifying nucleic acids or proteins comprising a plurality of piston pumps; and a plurality of nozzles capable of having a plurality of disposable tips which are automatically attachable/detachable. The apparatus includes a mechanism for mixing a liquid mixture of each sample and a reagent in a plurality of sections of a container by sucking up a plurality of the mixtures into tips, followed by discharging the mixtures in the sections simultaneously. The apparatus also includes a mechanism for dispensing a desired amount of a reagent to be used subsequently into the same number of sections of a different container as samples, while the mixing is in progress.
대표청구항▼
What is claimed is: 1. A method for purifying nucleic acids using magnetically attractable particles, the method comprising the steps of: adding magnetically attractable particles to a cell culture solution or suspension to collect the cells; adding a reagent for dissolving the cells to make conta
What is claimed is: 1. A method for purifying nucleic acids using magnetically attractable particles, the method comprising the steps of: adding magnetically attractable particles to a cell culture solution or suspension to collect the cells; adding a reagent for dissolving the cells to make contaminants other than an objective nucleic acid adsorb onto said particles; separating a supernatant from said particles; and adding additional magnetically attractable particles to the supernatant. 2. The method for purifying nucleic acids or proteins according to claim 1, wherein dispensing is carried out alternately with normal rotation and reverse rotation of rollers. 3. A method for purifying a plurality of samples of nucleic acids or proteins, the method comprising: providing an apparatus comprising: a plurality of piston pumps; and a plurality of nozzles capable of having automatically attachable or detachable disposable tips, the plurality of nozzles in communication with the plurality of piston pumps; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a first reagent; purifying each of the plurality of samples by sucking up waste from each of the plurality of liquid mixtures into respective ones of the tips using respective ones of the plurality of piston pumps and respective ones of the plurality of nozzles, and discharging the waste into respective ones of a plurality of sections of a first container, each of the plurality of sections having a bottom surface inclined in a vertical cross-sectional view and defining a hole that has a diameter that is smaller than the outer diameter of one of the tips near a center of the bottom surface; and dispensing a second reagent to be used subsequently into a same number of a plurality of sections of a second container while the purifying is in progress. 4. The method for purifying nucleic acids or proteins according to claim 3, wherein magnetically attractable particles are used. 5. The method for purifying nucleic acids or proteins according to claim 4, wherein the suspension of the magnetically attractable particles and a liquid are mixed by decreasing the pressure inside the container containing said suspension and discharging a certain amount of said suspension with the application of pressure greater than atmospheric pressure. 6. The method for purifying nucleic acids or proteins according to claim 3, wherein dispensing is carried out alternately with normal rotation and reverse rotation of rollers. 7. A method for purifying a plurality of samples comprising nucleic acids or proteins, the method comprising: providing an apparatus comprising: 96 piston pumps; and 96 nozzles capable of have 96 automatically attachable or detachable tips, the 96 nozzles in communication with the 96 piston pumps; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a first reagent; purifying each of the plurality of samples by sucking up waste from each of the plurality of liquid mixtures into respective ones of the tips using respective ones of the 96 piston pumps and respective ones of the 96 nozzles, and discharging the waste into respective ones of 96 sections of a first container, each of the respective ones of the 96 sections having a bottom surface inclined in a vertical cross-sectional view and defining a hole that has a diameter that is smaller than the outer diameter of one of the tips near a center of the bottom surface; and dispensing a second reagent to be used subsequently into a same number of a second container having 96 sections or a multiple of 96 sections while the purifying is in progress. 8. The method for purifying nucleic acids or proteins according to claim 7, wherein magnetically attractable particles are used. 9. The method for purifying nucleic acids or proteins according to claim 8, wherein the suspension of the magnetically attractable particles and a liquid are mixed by decreasing the pressure inside the container containing said suspension and discharging a certain amount of said suspension with the application of pressure greater than atmospheric pressure. 10. The method for purifying nucleic acids or proteins according to claim 7, wherein dispensing is carried out alternately with normal rotation and reverse rotation of rollers. 11. A method for purifying nucleic acids or proteins, the method comprising: providing 96 sections of a container, each of the 96 sections containing a suspension of magnetically attractable particles and a supernatant that is separable from the magnetically attractable particles using a magnetic force, each of the 96 sections having a bottom surface inclined in a vertical cross-sectional view and defining a hole that has a diameter that is smaller than the outer diameter of one of the tips near a center of the bottom surface; removing each supernatant from each suspension of the 96 sections using one of 96 hollow cylindrical magnets; and cooling or heating the container through the 96 magnets. 12. The method for purifying nucleic acids or proteins according to claim 11, wherein magnetically attractable particles are used. 13. The method for purifying nucleic acids or proteins according to claim 12, wherein the suspension of the magnetically attractable particles and a liquid are mixed by decreasing the pressure inside the container containing said suspension and discharging a certain amount of said suspension with the application of pressure greater than atmospheric pressure. 14. The method for purifying nucleic acids or proteins according to claim 11, wherein dispensing is carried out alternately with normal rotation and reverse rotation of rollers. 15. A method for purifying a plurality of samples of nucleic acids or proteins comprising: providing an apparatus comprising: a drive mechanism for a plurality of piston pumps; a mechanism for driving the drive mechanism up and down; magnets; a heating device directing heat; and a cooling device directing cold in a direction that the drive mechanism is driven, the directing of heat and the directing of cold being through the magnets, the drive mechanism, the heating device, the cooling device, and the magnets being vertically aligned; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a reagent; and purifying each of the plurality of samples by sucking up waste from each of the plurality of samples into respective ones of the tips and discharging the waste into respective ones of 96 sections of a container, each of the respective ones of the 96 sections having a bottom surface inclined in a vertical cross-sectional view and defining a hole that has a diameter that is smaller than the outer diameter of one of the tips near a center of the bottom surface. 16. The method for purifying nucleic acids or proteins according to claim 15, wherein magnetically attractable particles are used. 17. The method for purifying nucleic acids or proteins according to claim 16, wherein the suspension of the magnetically attractable particles and a liquid are mixed by decreasing the pressure inside the container containing said suspension and discharging a certain amount of said suspension with the application of pressure greater than atmospheric pressure. 18. The method for purifying nucleic acids or proteins according to claim 11, wherein dispensing is carried out alternately with normal rotation and reverse rotation of rollers. 19. A method for purifying a plurality of samples comprising nucleic acids or proteins, the method comprising: providing an apparatus comprising: a drive mechanism for a plurality of piston pumps; a mechanism for driving said drive mechanism up and down; a plate located directly beneath said plurality of piston pumps, the plate comprising a plurality of magnets having a similar arrangement to that of the plurality of piston pumps; a mechanism capable of heating and cooling under said plate; and a mechanism for conveying a container containing the plurality of samples between the plurality of piston pumps and the plate; and purifying each of the plurality of samples by sucking up waste from each of the plurality of samples into respective ones of the tips and discharging the waste into respective ones of 96 sections of a container, each of the respective ones of the 96 sections having a bottom surface inclined in a vertical cross-sectional view and defining a hole that has a diameter that is smaller than the outer diameter of one of the tips near a center of the bottom surface. 20. The method for purifying nucleic acids or proteins according to claim 19, wherein magnetically attractable particles are used. 21. The method for purifying nucleic acids or proteins according to claim 20, wherein the suspension of the magnetically attractable particles and a liquid are mixed by decreasing the pressure inside the container containing said suspension and discharging a certain amount of said suspension with the application of pressure greater than atmospheric pressure. 22. The method for purifying nucleic acids or proteins according to claim 19, wherein dispensing is carried out alternately with normal rotation and reverse rotation of rollers. 23. A method for purifying a plurality of samples comprising nucleic acids or proteins, the method comprising: providing an apparatus comprising: a plurality of piston pumps; a plurality of nozzles capable of having automatically attachable or detachable disposable tips, the plurality of nozzles in communication with the plurality of piston pumps; and a mechanism for purifying each of the plurality of samples by sucking waste from each of the plurality of samples into respective ones of the tips and discharging the waste into respective ones of a plurality of sections of a container, the plurality of sections having an arrangement corresponding to the format of the plurality of piston pumps; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a reagent; and purifying each of the plurality of samples by sucking up waste from each of the plurality of samples into respective ones of the tips and discharging the waste into respective ones of the plurality of sections of the container, each of the respective ones of the plurality of sections having a bottom surface inclined in a vertical cross-sectional view and defining a hole that has a diameter that is smaller than the outer diameter of one of the tips near a center of the bottom surface. 24. The method for purifying nucleic acids or proteins according to claim 23, wherein magnetically attractable particles are used. 25. The method for purifying nucleic acids or proteins according to claim 24, wherein the suspension of the magnetically attractable particles and a liquid are mixed by decreasing the pressure inside the container containing said suspension and discharging a certain amount of said suspension with the application of pressure greater than atmospheric pressure. 26. A method for purifying a plurality of samples comprising nucleic acids or proteins, the method comprising: providing an apparatus comprising: a plurality of piston pumps; and a plurality of nozzles capable of having automatically attachable or detachable tips, the plurality of nozzles in communication with the plurality of piston pumps; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a first reagent; mixing each of the plurality of liquid mixtures by sucking up each of the plurality of liquid mixtures into respective ones of the tips and discharging simultaneously each of the plurality of liquid mixtures into respective ones of a plurality of sections of a first container; and dispensing a second reagent to be used subsequently into a same number of a plurality of sections of a second container while the purifying is in progress, wherein dispensing comprises using a plurality of roller tube pumps, each of the plurality of roller tube pumps being connected to tubes having different diameters, and a reagent inlet and a reagent outlet of each of the tubes being common. 27. A method for purifying a plurality of samples comprising nucleic acids or proteins, the method comprising: providing an apparatus comprising: 96 piston pumps; and 96 nozzles capable of have 96 automatically attachable or detachable tips, the 96 nozzles in communication with the 96 piston pumps; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a first reagent; mixing each of the plurality of liquid mixtures by sucking up each of the plurality of liquid mixtures into respective ones of the tips and discharging simultaneously each of the plurality of liquid mixtures into respective ones of 96 sections of a first container; and dispensing a second reagent to be used subsequently into a same number of sections of a second container having 96 sections while the mixing is in progress, wherein dispensing comprises using a plurality of roller tube pumps, each of the plurality of roller tube pumps being connected to tubes having different diameters, and a reagent inlet and a reagent outlet of each of the tubes being common. 28. A method for purifying nucleic acids or proteins, comprising: providing 96 sections of a container, each of the 96 sections containing a suspension of magnetically attractable particles and a supernatant that is separable from the magnetically attractable particles using a magnetic force; removing each supernatant from each suspension of the 96 sections using one of 96 hollow cylindrical magnets; cooling or heating the container through the 96 magnets; and using one of a plurality of roller tube pumps for removing each supernatant, each of the plurality of roller tube pumps being connected to tubes having different diameters, and a reagent inlet and a reagent outlet of each of the tubes being common. 29. A method for purifying nucleic acids or proteins comprising: providing an apparatus comprising: a drive mechanism for a plurality of piston pumps; a mechanism for driving the drive mechanism up and down; magnets; a heating device directing heat; and a cooling device directing cold in a direction that the drive mechanism is driven, the directing of heat and the directing of cold being through the magnets; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a reagent; and using a plurality of roller tube pumps for transferring and dispensing the reagent from each of the plurality of liquid mixtures, each roller tube pump being connected to tubes having different diameters, and a reagent inlet and a reagent outlet of each tube being common. 30. A method for purifying a plurality of samples comprising nucleic acids or proteins, the method comprising: providing an apparatus comprising: a drive mechanism for a plurality of piston pumps; a mechanism for driving said drive mechanism up and down; a plate located directly beneath said plurality of piston pumps, the plate comprising a plurality of magnets having a similar arrangement to that of the plurality of piston pumps; a mechanism capable of heating and cooling under said plate; and a mechanism for conveying a container containing the plurality of samples between the plurality of piston pumps and the plate; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a reagent; purifying each of the plurality of samples by sucking up waste from each of the plurality of liquid mixtures into respective ones of the tips and discharging the waste into a container; and using a plurality of roller tube pumps for transferring and dispensing the reagent from each of the plurality of liquid mixtures, each of the roller tube pumps being connected to tubes having different diameters, and a reagent inlet and reagent outlet of each of the tubes being common. 31. A method for purifying a plurality of samples comprising nucleic acids or proteins, the method comprising: providing an apparatus comprising: a plurality of piston pumps; and a plurality of nozzles capable of having automatically attachable or detachable disposable tips, the plurality of nozzles in communication with the plurality of piston pumps; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a reagent; purifying each of the plurality of samples by sucking up waste from each of the plurality of samples into respective ones of the tips and discharging the waste into respective ones of a plurality of sections of a container, the plurality of sections having an arrangement corresponding to the format of the plurality of piston pumps; and using a plurality of roller tube pumps for transferring and dispensing the reagents, each of the plurality of roller tube pumps being connected to tubes having different diameters, and a reagent inlet and reagent outlet of each of the tubes being common. 32. A method for purifying a plurality of samples of nucleic acids or proteins, the method comprising: providing an apparatus comprising; a plurality of piston pumps; and a plurality of nozzles having detachable tips thereon, the plurality of nozzles in communication with the plurality of piston pumps; providing a plurality of liquid mixtures, each of the plurality of liquid mixtures comprising one of the plurality of samples and a first reagent; purifying each of the plurality of samples by sucking up waste from each of the plurality of liquid mixtures into respective ones of the tips using respective ones of the plurality of piston pumps and respective ones of the plurality of nozzles, and discharging the waste into respective ones of a plurality of sections of a first container; and dispensing a second reagent to be used subsequently into a same number of a plurality of sections of a second container while the purifying is in progress.
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