Method for the chemical synthesis of oligonucleotides
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07H-021/00
C07H-019/04
C07H-019/00
C12N-011/14
C12N-011/00
G01N-033/551
C07K-001/00
출원번호
US-0887182
(2001-06-22)
발명자
/ 주소
Vargeese,Chandra
Shaffer,Christopher
Wang,Weimin
출원인 / 주소
Sirna Therapeutics, Inc.
대리인 / 주소
McDonnell Boehnen Hulbert &
인용정보
피인용 횟수 :
20인용 특허 :
27
초록
The present invention features novel compositions, linkers, derivatized solid supports, and methods for the efficient solid phase synthesis of oligonucleotides, including RNA, DNA, RNA-DNA chimeras, and analogs thereof.
대표청구항▼
What is claimed is: 1. A compound of the Formula I: wherein SP is a solid support, W is a succinyl linkage, and B represents a terminal chemical group from which an oligonucleotide can be synthesized. 2. The compound of claim 1, wherein said B comprises a nucleic acid, nucleoside, nucleotide, o
What is claimed is: 1. A compound of the Formula I: wherein SP is a solid support, W is a succinyl linkage, and B represents a terminal chemical group from which an oligonucleotide can be synthesized. 2. The compound of claim 1, wherein said B comprises a nucleic acid, nucleoside, nucleotide, or an abasic moiety. 3. The compound of claim 2, wherein said nucleic acid, nucleoside, nucleotide, or abasic moiety comprises an acid labile protecting group. 4. The compound of claim 3, wherein said acid labile protecting group is a dimethoxytrityl, monomethoxytrityl, or trityl group. 5. A method of synthesizing a compound of claim 1, comprising: coupling a terminal chemical group comprising a nucleic acid, nucleoside, nucleotide, or an abasic moiety to the primary amine of a compound of Formula V(a): V(a) to form said compound of claim 1. 6. The method of claim 5, wherein said coupling is at a loading from about 50 to about 100 μmol/gram of said SP. 7. The method of claim 5, wherein said coupling is at a loading of about 75 to about 85 μmol/gram of said SP. 8. The compound of claim 1, wherein said SP is a controlled pore glass support. 9. The compound of claim 2, wherein said B is an abasic moiety. 10. The compound of claim 1, wherein said B is selected from adenosine, cytidine, guanosine, thymidine, or uridine. 11. The compound of claim 9, wherein said abasic succinate is a 5'-O-succinyl-3'-O-DMT deoxyribose. 12. The compound of claim 10, wherein said adenosine succinate is a 5'-O-DMT-3'-O-succinyl adenosine with or without nitrogen protecting groups. 13. The compound of claim 10, wherein said cytidine succinate is a 5'-O-DMT-3'-O-succinyl cytidine with or without nitrogen protecting groups. 14. The compound of claim 10, wherein said guanosine succinate is a 5'-O-DMT-3'-O-succinyl guanosine with or without nitrogen protecting groups. 15. The compound of claim 10, wherein said thymidine succinate is a 5'-O-DMT-3'-O-succinyl thymidine. 16. The compound of claim 10, wherein said uridine succinate is a 5'-O-DMT-3'-O-succinyl uridine.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (27)
Beigelman Leonid ; Karpeisky Alex, 2'-O-alkylthioalkyl and 2-C-alkylthioalkyl-containing enzymatic nucleic acids (ribozymes).
Kobayashi Hideki (Ichihara JPX) Matsunaga Tadashi (Fuchu MI JPX) Kobayashi Hideki (Midland MI), Immobilization of physiologically active substances on an inorganic support.
Eckstein Fritz (Gottingen DEX) Pieken Wolfgang (Boulder CO) Benseler Fritz (Gleichen/Etzborn DEX) Olsen David B. (West Point PA) Williams David M. (Cambridge GB2) Heidenreich Olaf (Gottingen DEX), Modified ribozymes.
Cech Thomas R. (Boulder CO) Zaug Arthur J. (Louisville CO) Been Michael D. (Boulder CO), RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods.
Reed Michael W. (Seattle WA) Meyer ; Jr. Rich B. (Woodinville WA) Petrie Charles R. (Woodinville WA) Tabone John C. (Bothell WA), Solid support for synthesis of 3′-tailed oligonucleotides.
Rother, Russell P.; Sheridan, Douglas L.; Tamburini, Paul P.; Zhang, Yuchun, Methods of treating complement-associated disorders with anti-C5a antibodies.
Rother, Russell P.; Sheridan, Douglas L.; Tamburini, Paul P.; Zhang, Yuchun, Methods of treating complement-associated disorders with anti-C5a antibodies.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.