초록

A gene encoding diacylglycerol acyltransferase from Brassica napus is described, as well as methods of altering oil content in plants using the diacylglycerol acyltransferase.

대표청구항 ▼

What is claimed is: 1. An isolated nucleic acid encoding a diacylglycerol acyltransferase polypeptide, wherein said nucleic acid is from the insert of pMB143, having American Type Culture Accession number PTA-4937. 2. An expression vector comprising said nucleic acid of claim 1 operably linked to

What is claimed is: 1. An isolated nucleic acid encoding a diacylglycerol acyltransferase polypeptide, wherein said nucleic acid is from the insert of pMB143, having American Type Culture Accession number PTA-4937. 2. An expression vector comprising said nucleic acid of claim 1 operably linked to an expression control element. 3. The expression vector of claim 2, wherein said expression control element is a constitutive promoter. 4. The expression vector of claim 2, wherein said expression control element is an inducible promoter. 5. The expression vector of claim 2, wherein said expression control element is a seed-specific promoter. 6. The expression vector of claim 5, wherein said seed-specific promoter is selected from the group consisting of the napin, phaseolin, oleosin, and cruciferin promoters. 7. The expression vector of claim 2, wherein said nucleic acid is operably linked to said expression control element in sense orientation. 8. A transgenic plant comprising said expression vector of claim 2. 9. The transgenic plant of claim 8, wherein said plant is a soybean plant. 10. The transgenic plant of claim 8, wherein said plant is a Brassica plant. 11. Seeds produced by said transgenic plant of claim 8. 12. Progeny of said transgenic plant of claim 8, wherein said progeny comprise said expression vector.