Methods and devices for thermal processing of multiple samples at the same time are disclosed. The sample processing devices provide process arrays that include conduits useful in distributing sample materials to a group pf process chambers located in fluid communication with the main conduits. The
Methods and devices for thermal processing of multiple samples at the same time are disclosed. The sample processing devices provide process arrays that include conduits useful in distributing sample materials to a group pf process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas.
대표청구항▼
What is claimed is: 1. A method of processing sample materials, the method comprising: providing a sample processing device that comprises: a body comprising a first side attached to a second side; a process array formed between the first and second sides, the process array comprising a loading st
What is claimed is: 1. A method of processing sample materials, the method comprising: providing a sample processing device that comprises: a body comprising a first side attached to a second side; a process array formed between the first and second sides, the process array comprising a loading structure, a main conduit comprising a length, a plurality of process chambers distributed along the main conduit, wherein the main conduit is in fluid communication with the loading structure and the plurality of process chambers; and a deformable seal located between the loading structure and the plurality of process chambers; distributing sample material to at least some of the process chambers through the main conduit; closing the deformable seal, wherein closing the deformable seal comprises deforming a deformable metallic layer; locating the body in contact with a thermal block; and controlling the temperature of the thermal block while the body is in contact with the thermal block. 2. A method according to claim 1, wherein closing the deformable seal comprises occluding the main conduit along substantially all the length of the main conduit. 3. A method according to claim 1, wherein closing the deformable seal comprises occluding only a portion of the length of the main conduit, wherein the portion is located between the loading structure and the plurality of process chambers. 4. A method according to claim 1, wherein closing the deformable seal comprises deforming a deformable portion of the second side of the body. 5. A method according to claim 1, wherein at least a portion of the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the adhesive. 6. A method according to claim 1, wherein at least a portion of the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the pressure sensitive adhesive. 7. A method according to claim 1, wherein the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the adhesive to occlude at least a portion of the length of the main conduit. 8. A method according to claim 1, wherein the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the pressure sensitive adhesive to occlude at least a portion of the length of the main conduit. 9. A method according to claim 1, wherein the loading structure comprises a loading chamber in fluid communication with the main conduit, and wherein distributing sample material to at least some of the process chambers through the main conduit further comprises providing the sample material in the loading chamber. 10. A method according to claim 1, wherein the loading structure comprises a loading chamber in fluid communication with the main conduit, wherein the loading chamber defines a loading chanter volume equal to or greater than a combined volume of the main conduit and the plurality of process chambers, and wherein distributing sample material is to at least some of the process chambers through the main conduit further comprises providing the sample material in the loading chamber. 11. A method according to claim 1, wherein each process chamber of the plurality of process chambers contains at least one reagent before the sample material is distributed. 12. A method according to claim 1, wherein at least a portion of each of the process chambers transmits electromagnetic energy of selected wavelengths. 13. A method of processing sample materials, the method comprising: providing a sample processing device that comprises: a body comprising a first side attached to a second side, wherein die second side comprises a metallic layer; and a process array formed between the first and second sides, the process array comprising a loading structure, a main conduit comprising a length, a plurality of process chambers distributed along the main conduit, wherein the main conduit is in fluid communication with the loading structure and the plurality of process chambers, a deformable seal located between the loading structure and the plurality of process chambers, wherein the deformable seal comprises pressure sensitive adhesive; distributing sample material to at least some of the process chambers through the main conduit; closing the deformable seal by deforming the metallic layer of the second side and adhering the first side and the second side together using the pressure sensitive adhesive; locating the body in contact with a thermal block; and controlling the temperature of the thermal block while the body is in contact with the thermal block. 14. A method of processing sample materials, the method comprising: providing a sample processing device that comprises: a body comprising a first side attached to a second side; and a process array formed between the first and second sides, the process array comprising a loading structure, a main conduit comprising a length, a plurality of process chambers distributed along the main conduit, wherein the main conduit is in fluid communication with the loading structure and the plurality of process chambers; and a deformable seal located between the loading structure and the plurality of process chambers; distributing sample material to at least some of the process chambers through the main conduit; closing the deformable seal; separating the loading structure from the sample processing device after closing the deformable seal; locating the body in contact with a thermal block; and controlling the temperature of the thermal block while the body is in contact with the thermal block. 15. A method according to claim 14, wherein closing the deformable seal comprises occluding the main conduit along substantially all of the length of the main conduit. 16. A method according to claim 14, wherein closing the deformable seal comprises occluding the main conduit along substantially all of the length of the main conduit beginning at a point distal from the loading structure and proceeding towards the loading structure, whereby sample material within the main conduit is urged towards the loading structure. 17. A method according to claim 14, wherein closing the deformable seal comprises occluding the main conduit along only a portion of the length of the main conduit, wherein the portion of the main conduit is located between the loading structure and the plurality of process chambers. 18. A method according to claim 14, wherein closing the deformable seal comprises occluding the main conduit along at least a portion of the length of the main conduit, wherein the portion of the main conduit is located between the loading structure and the plurality of process chambers; and further wherein separating the loading structure comprises separating the body along a line extending through the portion of the main conduit occluded during the closing of the deformable seal. 19. A method according to claim 14, wherein at least a portion of the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the adhesive. 20. A method according to claim 14, wherein at least a portion of the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the pressure sensitive adhesive; and further wherein closing the deformable seal comprises occluding the main conduit along substantially all of the length of the main conduit beginning at a point distal from the loading structure and proceeding towards the loading structure, whereby sample material within the main conduit is urged towards the loading structure. 21. A method according to claim 14, wherein the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the adhesive. 22. A method according to claim 14, wherein the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the pressure sensitive adhesive. 23. A method according to claim 14, wherein the loading structure comprises a loading chamber in fluid communication with the main conduit, and wherein distributing sample material to at least some of the process chambers through the main conduit further comprises providing the sample material in the loading chamber. 24. A method according to claim 14, wherein the loading structure comprises a loading chamber in fluid communication with the main conduit, wherein the loading chamber defines a loading chamber volume equal to or greater than a combined volume of the main conduit and the plurality of process chambers, and wherein distributing sample material to at least some of the process chambers through the main conduit further comprises providing the sample material in the loading chamber. 25. A method according to claim 14, wherein each process chamber of the plurality of process chambers contains at least one reagent before the sample material is distributed. 26. A method according to claim 14, wherein at least a portion of each of the process chambers transmits electromagnetic energy of selected wavelengths. 27. A method of processing sample materials, the method comprising: providing a sample processing device that comprises: a body comprising a first side attached to a second side, a plurality of process arrays formed between the first and second sides, wherein each process array of the plurality of process arrays comprises: a loading structure, a main conduit comprising a length, and a plurality of process chambers distributed along the main conduit, wherein the main conduit is in fluid communication with the loading structure and the plurality of process chambers, and a deformable seal located between the loading structure and the plurality of process chambers; distributing sample material to at least some of the process chambers in each process array of the plurality of process arrays through the main conduit in each of the process arrays; closing the deformable seal in each process array of the plurality of process arrays, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises deforming a deformable metallic layer; locating the body in contact with a thermal block; and controlling the temperature of the thermal block while the body is in contact with the thermal block. 28. A method according to claim 27, wherein closing the deformable seal in each process array of the plurality of process arrays comprises simultaneously closing the deformable seal in each process array of the plurality of process arrays. 29. A method according to claim 27, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises occluding the main conduit along substantially all of the length of the main conduit. 30. A method according to claim 27, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises occluding the main conduit along only a portion of the length of the main conduit, wherein the portion of the main conduit is located between the loading structure and the plurality of process chambers. 31. A method according to claim 27, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises deforming a deformable portion of the second side of the body. 32. A method according to claim 27, wherein, for each process array of the plurality of process arrays, at least a portion of the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the adhesive. 33. A method according to claim 27, wherein, for each process array of the plurality of process arrays, at least a portion of the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the pressure sensitive adhesive. 34. A method according to claim 27, wherein, for each process array of the plurality of process arrays, the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the adhesive to occlude the main conduit along at least a portion of the length of the main conduit. 35. A method according to claim 27, wherein, for each process array of the plurality of process arrays, the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the pressure sensitive adhesive to occlude the main conduit along at least a portion of the length of the main conduit. 36. A method according to claim 27, wherein, for each process array of the plurality of process arrays, each process chamber of die plurality of process chambers contains at least one reagent before the sample material is distributed. 37. A method according to claim 27, wherein, for each process array of the plurality of process arrays, at least a portion of each of the process chambers transmits electromagnetic energy of selected wavelengths. 38. A method according to claim 27, wherein, for each process array of the plurality of process arrays, the loading structure comprises a U-shaped loading chamber comprising first and second legs, an inlet port located proximate a distal end of the first leg, and a vent port located proximate a distal end of the second leg, and further wherein distributing sample material comprises delivering the sample material to the inlet port and moving the sample material to the main conduit from the loading structure. 39. A method of processing sample materials, the method comprising: providing a sample processing device that comprises: a body comprising a first side attached to a second side; a process array formed between the first and second sides, the process array comprising a loading structure, a main conduit comprising a length, a plurality of process chambers distributed along the main conduit, wherein the main conduit is in fluid communication with the loading structure and the plurality of process chambers, and wherein at least a portion of each of the process chambers transmits electromagnetic energy of selected wavelengths; and a deformable seal located between the loading structure and the plurality of process chambers; distributing sample material to at least some of the process chambers through the main conduit; closing the deformable seal; locating the body in contact with a thermal block; and controlling the temperature of the thermal block while the body is in contact with the thermal block. 40. A method according to claim 39, wherein closing the deformable seal comprises occluding the main conduit along substantially all the length of the main conduit. 41. A method according to claim 39, wherein closing the deformable seal comprises occluding only a portion of the length of the main conduit, wherein the portion is located between the loading structure and the plurality of process chambers. 42. A method according to claim 39, wherein closing the deformable seal comprises deforming a deformable portion of the second side of the body. 43. A method according to claim 39, wherein at least a portion of the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the adhesive. 44. A method according to claim 39, wherein at least a portion of the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the pressure sensitive adhesive. 45. A method according to claim 39, wherein the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the adhesive to occlude at least a portion of the length of the main conduit. 46. A method according to claim 39, wherein the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the pressure sensitive adhesive to occlude at least a portion of the length of the main conduit. 47. A method according to claim 39, wherein the loading structure comprises a loading chamber in fluid communication with the main conduit, and wherein distributing sample material to at least some of the process chambers through the main conduit further comprises providing the sample material in the loading chamber. 48. A method according to claim 39, wherein the loading structure comprises a loading chamber in fluid communication with the main conduit, wherein the loading chamber defines a loading chamber volume equal to or greater than a combined volume of the main conduit and the plurality of process chambers, and wherein distributing sample material to at least some of the process chambers through the main conduit further comprises providing the sample material in the loading chamber. 49. A method according to claim 39, wherein each process chamber of the plurality of process chambers contains at least one reagent before the sample material is distributed. 50. A method of processing sample materials, the method comprising: providing a sample processing device that comprises: a body comprising a first side attached to a second side, a plurality of process arrays formed between the first and second sides, wherein each process array of the plurality of process arrays comprises: a loading structure, a main conduit comprising a length, and a plurality of process chambers distributed along the main conduit, wherein the main conduit is in fluid communication with the loading structure and the plurality of process chambers, and wherein, for each process array of the plurality of process arrays, at least a portion of each of the process chambers transmits electromagnetic energy of selected wavelengths, and a deformable seal located between the loading structure and the plurality of process chambers; distributing sample material to at least some of the process chambers in each process array of the plurality of process arrays through the main conduit in each of the process arrays; closing the deformable seal in each process array of the plurality of process arrays; locating the body in contact with a thermal block; and controlling the temperature of the thermal block while the body is in contact with the thermal block. 51. A method according to claim 50, wherein closing the deformable seal in each process array of the plurality of process arrays comprises simultaneously closing the deformable seal in each process array of the plurality of process arrays. 52. A method according to claim 50, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises occluding the main conduit along substantially all of the length of the main conduit. 53. A method according to claim 50, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises occluding the main conduit along only a portion of the length of the main conduit, wherein the portion of the main conduit is located between the loading structure and the plurality of process chambers. 54. A method according to claim 50, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises deforming a deformable portion of the second side of the body. 55. A method according to claim 50, wherein, for each process array of the plurality of process arrays, at least a portion of the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the adhesive. 56. A method according to claim 50, wherein, for each process array of the plurality of process arrays, at least a portion of the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the pressure sensitive adhesive. 57. A method according to claim 50, wherein, for each process array of the plurality of process arrays, the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the adhesive to occlude the main conduit along at least a portion of the length of the main conduit. 58. A method according to claim 50, wherein, for each process array of the plurality of process arrays, the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the pressure sensitive adhesive to occlude rue main conduit along at least a portion of the length of the main conduit. 59. A method according to claim 50, wherein, for each process array of the plurality of process arrays, each process chamber of the plurality of process chambers contains at least one reagent before the sample material is distributed. 60. A method according to claim 50, wherein, for each process array of the plurality of process arrays, the loading structure comprises a U-shaped loading chamber comprising first and second legs, an inlet port located proximate a distal end of the first leg, and a vent port located proximate a distal end of the second leg, and further wherein distributing sample material comprises delivering the sample material to the inlet port and moving the sample material to the main conduit from the loading structure. 61. A method of processing sample materials, the method comprising: providing a sample processing device that comprises: a body comprising a first side attached to a second side, a plurality of process arrays formed between the first and second sides, wherein each process array of the plurality of process arrays comprises: a loading structure, a main conduit comprising a length, and a plurality of process chambers distributed along the main conduit, wherein the main conduit is in fluid communication with the loading structure and the plurality of process chambers, and a deformable seal located between the loading structure and the plurality of process chambers; distributing sample material to at least some of the process chambers in each process array of the plurality of process arrays through the main conduit in each of the process arrays; closing the deformable seal in each process array of the plurality of process arrays; locating the body in contact with a thermal block; and controlling the temperature of the thermal block while the body is in contact with the thermal block; wherein, for each process ray of the plurality of process arrays, the loading structure comprises a U-shaped loading chamber comprising first and second legs, an inlet port located proximate a distal end of the first leg, and a vent port located proximate a distal end of the second leg, and further wherein distributing sample material comprises delivering the sample material to the inlet port and moving the sample material to the main conduit from the loading structure. 62. A method according to claim 61, wherein closing the deformable seal in each process array of the plurality of process arrays comprises simultaneously closing the deformable seal in each process array of the plurality of process arrays. 63. A method according to claim 61, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises occluding the main conduit along substantially all of the length of the main conduit. 64. A method according to claim 61, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises occluding the main conduit along only a portion of the length of the main conduit, wherein the portion of the main conduit is located between the loading structure and the plurality of process chambers. 65. A method according to claim 61, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises deforming a deformable portion of the second side of the body. 66. A method according to claim 61, wherein, for each process array of the plurality of process arrays, closing the deformable seal comprises deforming a deformable metallic layer. 67. A method according to claim 61, wherein, for each process array of the plurality of process arrays, at least a portion of the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the adhesive. 68. A method according to claim 61, wherein, for each process array of the plurality of process arrays, at least a portion of the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together using the pressure sensitive adhesive. 69. A method according to claim 61, wherein, for each process array of the plurality of process arrays, the deformable seal comprises adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the adhesive to occlude the main conduit along at least a portion of the length of the main conduit. 70. A method according to claim 61, wherein, for each process array of the plurality of process arrays, the deformable seal comprises pressure sensitive adhesive, and wherein closing the deformable seal comprises adhering the first side and the second side together with the pressure sensitive adhesive to occlude the main conduit along at least a portion of the length of the main conduit. 71. A method according to claim 61, wherein, for each process array of the plurality of process arrays, each process chamber of the plurality of process chambers contains at least one reagent before the sample material is distributed. 72. A method according to claim 61, wherein, for each process array of the plurality of process arrays, at least a portion of each of the process chambers transmits electromagnetic energy of selected wavelengths.
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