초록

The present invention relates to nucleic acid sequences and amino acid sequences for Bacillus subtilis disulfide bond isomerases, Dsb1 and Dsb2 and methods for increasing the secretion of heterologous and homologous proteins in Gram-positive microorganisms.

대표청구항 ▼

The invention claimed is: 1. An expression vector comprising a nucleic acid encoding at least one Bacillus subtilis disulfide bond isomerase (Dsb) having an amino acic sequence selected from the group consisting of SEQ ID NOS:2 and 5, wherein said nucleic acid encoding disulfide bond isomerase is u

The invention claimed is: 1. An expression vector comprising a nucleic acid encoding at least one Bacillus subtilis disulfide bond isomerase (Dsb) having an amino acic sequence selected from the group consisting of SEQ ID NOS:2 and 5, wherein said nucleic acid encoding disulfide bond isomerase is under the control of expression signals that overexpress said disulfide bond isomerase in a Gram-positive bacterium. 2. The expression vector of claim 1, wherein said Gram-positive bacterium is a member of the genus Bacillus. 3. The expression vector of claim 2, wherein said member of the genus Bacillus is selected from the group consisting of B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. coagulans, B. circulans, B. lentus, and B. thuringiensis. 4. A Gram positive bacterial host cell comprising the expression vector of claim 1. 5. The Gram-positive bacterial host cell of claim 4, wherein said host cell is a member of the genus Bacillus. 6. The Gram-positive bacterial host cell of claim 5, wherein said member of the genus Bacillus is selected from the group consisting of B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. coagulans, B. circulans, B. lentus and B. thuringiensis. 7. The Gram-positive bacterial host cell of claim 5, wherein said host cell expresses at least one heterologous protein. 8. The Gram-positive bacterial host cell of claim 7, wherein said heterologous protein is selected from the group consisting of hormones, enzymes, growth factors, and cytokines. 9. The Gram-positive bacterial host cell of claim 8, wherein said heterologous protein is an enzyme. 10. The Gram-positive bacterial host cell of claim 9, wherein said enzyme is selected from the group consisting of proteases, carbohydrases, reductases, lipases, isomerases, transferases, kinases, and phosphatases. 11. The expression vector of claim 1, comprising at least one nucleic acid having a nucleic acid sequence selected from the group consisting of SEQ ID NOS:1 and 4. 12. A Gram positive bacterial host cell comprising the expression vector of claim 11.