초록 ▼

Disclosed is a biochip image analysis system which comprises an image storage unit for storing images including an original image of a biochip comprising a set of specific genes expressed in different environments by fluorescent bases of different colors; an image converter for converting the origin

Disclosed is a biochip image analysis system which comprises an image storage unit for storing images including an original image of a biochip comprising a set of specific genes expressed in different environments by fluorescent bases of different colors; an image converter for converting the original image into a test image, forming an overlapped image and a color image, and storing the images in the image storage unit; an edge detector for separating the spot-formed genes from the test image, generating spot and background templates, and detecting an edge to measure expression degrees of the genes; a data processor for generating statistical data using the spot and background templates, and performing luminance correction; and a data storage unit for storing the statistical data.

대표청구항 ▼

What is claimed is: 1. A biochip image analysis method, comprising: (a) extracting an original image according to a fluorescent base from a biochip comprising a set of specific genes each expressed in a different environment by a fluorescent base of a different color; (b) converting the original i

What is claimed is: 1. A biochip image analysis method, comprising: (a) extracting an original image according to a fluorescent base from a biochip comprising a set of specific genes each expressed in a different environment by a fluorescent base of a different color; (b) converting the original image into a test image, and extracting an overlapped image and a color image from the test image; (c) respectively separating the genes in the spot form from the test image, generating a spot template and a background template including an area within a predetermined distance excluding the spot, detecting an edge for enhancing the luminance of the test image to emphasize the edge for comparing a previously established critical value with a mean value of a predetermined number of pixels from the edge emphasized in the enhancing step, to generate an edge, extracting an effective edge for removing edges under a reference size from the edges detected in the comparing step, and connecting partially opened edges by the shortest distance and storing a template for generating a spot template and a background template comprising the edges extracted in the effective edge extraction step, and storing the spot template and the background template, and (d) outputting statistical data on the basis of the spot template and the background template generated in (b) or (c), and performing luminance correction. 2. A biochip image analysis method, comprising: (a) extracting an original image according to a fluorescent base from a biochip comprising a set of specific genes each expressed in a different environment by a fluorescent base of a different color; (b) converting the original image into a test image, and extracting an overlapped image and a color image from the test image; (c) respectively separating the genes in the spot form from the test image, generating a spot template and a background template including an area within a predetermined distance excluding the spot, detecting an edge for generating a reference template on the center of which a reference circle is formed on the basis of a mean size of the spots from the test image; an enhancing step for enhancing the luminance of the test image to emphasize the edge for comparing a previously established critical value with a mean value of a predetermined number of pixels from the edge emphasized in the enhancing step, to generate an edge, extracting an effective edge for removing edges under a reference size from the edges detected in the comparing step, and connecting partially opened edges by the shortest distance to generate an effective edge template storing a template for synthesizing the effective edge template and the reference template to generate a spot template, synthesizing the inverted effective edge template and the inverted reference template to generate a background template, and storing the spot template and the background template, and (d) outputting statistical data on the basis of the spot template and the background template generated in (b) or (c), and performing luminance correction. 3. The method of claim 1, wherein the comparison step comprises a smoothing step for reducing the gradient of the edge emphasized in the enhancement step to extract effective edges. 4. The method of claim 2, wherein the comparison step comprises a smoothing step for reducing the gradient of the edge emphasized in the enhancement step to extract effective edges. 5. A biochip image analysis method, comprising: (a) extracting an original image according to a fluorescent base from a biochip comprising a set of specific genes each expressed in a different environment by a fluorescent base of a different color; (b) converting the original image into a test image, and extracting an overlapped image and a color image from the test image; (c) respectively separating the genes in the spot form from the test image, generating a spot template and a background template including an area within a predetermined distance excluding the spot, and detecting an edge; and (d) outputting statistical data on the basis of the spot template and the background template generated in (b) or (c), and performing luminance correction, wherein in (d), various measured values including a mean value, a standard deviation, a central value, a mode, a spot's area and circumference, a number of holes in the spot, and a fragmentation state are stored as statistical data on the basis of the spot template and the background template.