IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0180219
(2002-06-26)
|
발명자
/ 주소 |
- Boga,Rameshbabu
- Chidebelu Eze,Chibueze Obinna
- Kaylor,Rosann M.
- Song,Xuedong
|
출원인 / 주소 |
- Kimberly Clark Worldwide, Inc.
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
13 인용 특허 :
177 |
초록
▼
An enhanced diffraction based biosensor system and method are provided for detecting an analyte of interest in a test medium. The system incorporates at least one additional detection tag substance with the analyte of interest, the tag emitting a measurable parameter that is different from optical d
An enhanced diffraction based biosensor system and method are provided for detecting an analyte of interest in a test medium. The system incorporates at least one additional detection tag substance with the analyte of interest, the tag emitting a measurable parameter that is different from optical diffraction characteristics of the analyte. The biosensor may be a "fluoroptical" system wherein the detection tag is a fluorescence emitting substance, including fluorescent-labeled diffraction enhancing elements. The enhanced diffraction biosensor system may determine the presence of analytes in biological fluids both qualitatively and quantitatively.
대표청구항
▼
What is claimed is: 1. A method of detecting an analyte of interest in a medium with a diffraction based biosensor having at least one additional detection tag mechanism, comprising: exposing a detection tag material to the medium, the detection tag material tagging the analyte of interest with a m
What is claimed is: 1. A method of detecting an analyte of interest in a medium with a diffraction based biosensor having at least one additional detection tag mechanism, comprising: exposing a detection tag material to the medium, the detection tag material tagging the analyte of interest with a measurable parameter in addition to optical diffraction characteristics of the analyte of interest; exposing a biosensor to the medium, the biosensor having a substrate member with a receptive material layer applied to at least one side thereof wherein the receptive material is specific for the analyte of interest such that the analyte binds to the receptive material; separating an unbound detection tag material from a bound detection tag material tagging the analyte of interest; exposing the biosensor to a light source and detecting the presence of the analyte by detecting a diffraction pattern of the transmitted light or reflected light; and detecting the emitted measurable parameter of the detection tag material. 2. The method as in claim 1, wherein the detection tag material is a fluorescence emitting substance. 3. The method as in claim 2, wherein the amount of fluorescence emitted by the substance is quantitatively measured. 4. The method as in claim 2, wherein the fluorescence emitting substance is fluorescent-labeled diffraction enhancing elements having a receptor material specific for the analyte of interest such that the elements bind to the analyte of interest in the medium. 5. The method as in claim 4, wherein the fluorescent-labeled diffraction enhancing elements are beads. 6. The method as in claim 2, wherein the fluorescence emitting substance is a fluorescent dye or tracer that stains the analyte of interest in the medium. 7. The method as in claim 1, wherein the detection tag material is one of a color-based, radiation-based, and chemical induced luminescence-based substance. 8. The method as in claim 1, wherein the detection tag material is added to the medium prior to exposing the biosensor to the medium. 9. The method as in claim 8, wherein the detection tag material is fluorescent-labeled diffraction enhancing elements that bind to the analyte of interest in the medium. 10. The method as in claim 1, wherein the detection tag material is added to the medium after exposing the biosensor to the medium. 11. The method as in claim 10, wherein the detection tag material is fluorescent-labeled diffraction enhancing elements that bind to the analyte of interest after the analyte has bound to the receptive material on the substrate. 12. The method as in claim 1, wherein the detection tag material is added to the medium simultaneously with exposing the biosensor to the medium. 13. The method as in claim 12, wherein the detection tag material is fluorescent-labeled diffraction enhancing elements that bind to the analyte of interest in the medium and the analytes which have already been bound to the receptive material on the substrate. 14. The method as in claim 1, comprising selecting the substrate member from the group of materials consisting of plastics, metal coated plastics and glass, functionalized plastics and glass, silicon wafers, metal oxides, glass, and foils. 15. The method as in claim 14 wherein the substrate member comprises a polymer film coated with a metal. 16. The method as in claim 15, wherein the polymer film comprises polyethylene-terephthalate. 17. The method as in claim 15, comprising selecting the metal from the group consisting of gold, silver, chromium, nickel, platinum, aluminum, iron, copper, gold oxide, titanium, chromium oxide, silver oxide, and zirconium. 18. The method as in claim 1, comprising viewing the diffraction pattern with the naked eye or a viewing device. 19. The method as in claim 1, comprising selecting the receptive material from at least one of antigens, antibodies, nucleotides, chelators, enzymes, bacteria, yeasts, fungi, viruses, bacterial pili, bacterial flagellar materials, nucleic acids, polysaccharides, lipids, proteins, carbohydrates, metals, hormones, peptides, aptamers, and respective receptors for said materials. 20. The method as in claim 1, wherein the analyte of interest is selected from at least one of a bacteria, yeast, fungus, virus, rheumatoid factor, IgG, IgM, IgA, IgD, and IgE antibodies, carcinoembryonic antigen, streptococcus Group A antigen, viral antigens, antigens associated with autoimmune disease, allergens, tumor antigens, streptococcus group B antigen, HIV I or HIV II antigen, antibodies viruses, antigens specific to RSV, an antibody, antigen, enzyme, hormone, polysaccharide, protein, lipid, carbohydrate, drug, nucleic acid, Neisseria meningitides groups A, B, C, Y and W sub 135, Streptococcus pneumoniae, E. coli K1, Haemophilus influenza type A/B, an antigen derived from microorganisms, PSA and CRP antigens, a hapten, a drug of abuse, a therapeutic drug, environmental agents, or antigens specific to Hepatitis. 21. The method as in claim 1, wherein the receptive material is printed in a pattern on the substrate member, and further comprising applying a blocking material to non-printed areas of the substrate member.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.