Transgenic fiber producing plants with increased expression of sucrose phosphate synthase
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-015/05
C12N-015/11
C12N-015/82
출원번호
US-0217700
(2002-08-12)
발명자
/ 주소
Haigler,Candace H.
Holaday,A. Scott
출원인 / 주소
Texas Tech University
대리인 / 주소
Nixon Peabody LLP
인용정보
피인용 횟수 :
8인용 특허 :
17
초록▼
The present invention relates to a method of controlling the cellulose synthesis in plants to optimize the level of production and quality of the products derived from the plant. In particular, the present invention provides a transgenic cotton plant that has higher yields of cotton fiber and seed.
The present invention relates to a method of controlling the cellulose synthesis in plants to optimize the level of production and quality of the products derived from the plant. In particular, the present invention provides a transgenic cotton plant that has higher yields of cotton fiber and seed. The invention also provides methods for increasing the quality of cotton fiber produced from a cotton plant. The invention also provides general methods of changing the ratio of cellulose to other dry weight components of the plant, for changing the thickness of cell walls, for increasing the yield and changing the quality of other plant fibers, for increasing seed yield, and for increasing the tolerance of photosynthetic efficiency to cool night temperatures.
대표청구항▼
What is claimed: 1. A method of increasing the ratio of cellulose to total dry weight components of a cotton plant, comprising: introducing into a cotton plant a chimeric DNA construct comprising a plant specific promoter and a DNA encoding a sucrose phosphate synthase, said DNA construct expressin
What is claimed: 1. A method of increasing the ratio of cellulose to total dry weight components of a cotton plant, comprising: introducing into a cotton plant a chimeric DNA construct comprising a plant specific promoter and a DNA encoding a sucrose phosphate synthase, said DNA construct expressing sucrose phosphate synthase and increasing sucrose phosphate synthase activity relative to a non-transgenic cotton plant in an amount sufficient to increase the ratio of cellulose to total dry weight components of the transgenic cotton plant relative to a non-transgenic cotton plant. 2. The method according to claim 1, further comprising: growing said cotton plant. 3. The method according to claim 1, wherein the sucrose phosphate synthase is selected from the group consisting of spinach, Arabidopsis, beet, bean, citrus, maize, moss, potato, rice, sugar cane, and Synechocystis sucrose phosphate synthase. 4. The method according to claim 3, wherein the sucrose phosphate synthase is spinach sucrose phosphate synthase. 5. The method according to claim 1, wherein the chimeric DNA construct comprises a plant specific transcription initiation region. 6. The method according to claim 5, wherein the transcription initiation region is tissue specific. 7. The method according to claim 5, wherein the transcription initiation region is leaf specific. 8. The method according to claim 5, wherein the transcription initiation region is a RUBISCO small subunit promoter, a 35S promoter, a fiber enhanced promoter, a vascular cell enhanced promoter, a stem cell enhanced promoter, or a seed enhanced promoter. 9. The method according to claim 1, wherein the chimeric DNA construct is stably integrated into the genome of the plant. 10. The method according to claim 1, wherein said introducing of the chimeric DNA construct into the plant is carried out by a method selected from the group consisting of electroporation, Agrobacterium mediated transformation, biolistic gene transformation, chemically mediated transformation, and microinjection. 11. The method according to claim 1, wherein the increase in cellulose ratio occurs in xylem cells. 12. The method according to claim 1, wherein the increase in cellulose ratio occurs in phloem cells. 13. A method of increasing in a cotton plant the tolerance of photosynthetic efficiency to cool night temperatures measured by CO2 consumption, comprising: introducing into a cotton plant a chimeric DNA construct comprising a plant specific promoter and a DNA encoding a sucrose phosphate synthase, said DNA construct expressing sucrose phosphate synthase and increasing sucrose phosphate synthase activity relative to a non-transgenic cotton plant in an amount sufficient to increase tolerance of photosynthetic efficiency to cool night temperatures in the transgenic cotton plant relative to a non-transgenic cotton plant. 14. The method according to claim 13, further comprising: growing said cotton plant. 15. The method according to claim 13, wherein the sucrose phosphate synthase is selected from the group consisting of spinach, Arabidopsis, beet, bean, citrus, maize, moss, potato, rice, sugar cane, and Synechocystis sucrose phosphate synthase. 16. The method according to claim 15, wherein the sucrose phosphate synthase is spinach sucrose phosphate synthase. 17. The method according to claim 13, wherein the chimeric DNA construct comprises a plant specific transcription initiation region. 18. The method according to claim 17, wherein the transcription initiation region is tissue specific. 19. The method according to claim 17, wherein the transcription initiation region is leaf specific. 20. The method according to claim 17, wherein the transcription initiation region is a RUBISCO small subunit promoter, a 35S promoter, a fiber enhanced promoter, a vascular cell enhanced promoter, a stem cell enhanced promoter, or a seed enhanced promoter. 21. The method according to claim 13, wherein the chimeric DNA construct is stably integrated into the genome of the plant. 22. The method according to claim 13, wherein said introducing of the chimeric DNA construct into the plant is carried out by a method selected from the group consisting of electroporation, Agrobacterium mediated transformation, biolistic gene transformation, chemically mediated transformation, and microinjection. 23. A method of increasing the thickness of cell walls in a cotton plant, comprising: introducing into a cotton plant a chimeric DNA construct comprising a plant specific promoter and a DNA encoding a sucrose phosphate synthase, said DNA construct expressing sucrose phosphate synthase and increasing sucrose phosphate synthase activity relative to a non-transgenic cotton plant in an amount sufficient to increase the thickness of cell walls in the transgenic cotton plant relative to a non-transgenic plant. 24. The method according to claim 23 further comprising: growing said plant. 25. The method according to claim 23, wherein the sucrose phosphate synthase is selected from the group consisting of spinach, Arabidopsis, beet, bean, citrus, maize, moss, potato, rice, sugar cane, and Synechocystis sucrose phosphate synthase. 26. The method according to claim 25, wherein the sucrose phosphate synthase is spinach sucrose phosphate synthase.
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이 특허에 인용된 특허 (17)
Houtz Robert L., Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltrans.
Van Assche Charles,FRX ; Lando Danielle,FRX ; Bruneau Jean Michel,FRX ; Voelker Toni Alois ; Gervais Monica,FRX, Modification of sucrose phosphate synthase in plants.
Secor Gary A. (Fargo ND) Borovkov Alexander Y. (Fargo ND) McClean Phillip E. (Fargo ND) Sowokinos Joseph R. (Grand Forks ND), Modulation of sugar content in plants.
Kasukabe Yoshihisa,JPX ; Fujisawa Koichi,JPX ; Nishiguchi Susumu,JPX ; Maekawa Yoshihiko,JPX ; Allen Randy Dale, Production of cotton fibers with improved fiber characteristics by treatment with brassinosteroids.
Van Assche Charles (Marseille FRX) Lando Danielle (Paris FRX) Bruneau Jean Michel (Paris FRX) Voelker Toni Alois (Davis CA) Gervais Monica (Saint-Leu-La-Foret FRX), Sucrose phosphate synthase (SPS), its process for preparation its cDNA, and utilization of cDNA to modify the expression.
Van Assche Charles,FRX ; Lando Danielle,FRX ; Bruneau Jean Michel,FRX ; Voelker Toni Alois ; Gervais Monica,FRX, Sucrose phosphate synthetase isolated from maize.
Barry Gerard F. (St. Louis MO) Edwards Janice W. (St. Louis MO) Kishore Ganesh M. (Chesterfield MO) Stark David M. (Fenton MO), Tomato fruit promoters.
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