초록

Reagents, methods and kits for denaturation of protein are provided.

대표청구항 ▼

I claim: 1. A formulation for denaturing proteins comprising: a lithium salt at a concentration of about 2.5-4.0 M, an alcohol at a concentration of about 25-40% v/v, and a citrate at a concentration of about 25-100 mM. 2. The formulation of claim 1, which formulation lacks EDTA. 3. The form

I claim: 1. A formulation for denaturing proteins comprising: a lithium salt at a concentration of about 2.5-4.0 M, an alcohol at a concentration of about 25-40% v/v, and a citrate at a concentration of about 25-100 mM. 2. The formulation of claim 1, which formulation lacks EDTA. 3. The formulation of claim 1, wherein the formulation lacks a chaotropic substance. 4. The method of claim 3, wherein the chaotropic substance is guanidinium salt, urea, ammonium, cesium, rubidium, potassium, or iodide salt. 5. The formulation of claim 1, wherein the lithium salt is lithium chloride or lithium bromide. 6. The formulation of claim 1, wherein the lithium salt is lithium chloride. 7. The formulation of claim 1, wherein the lithium salt is at a concentration of about 3.5 M. 8. The formulation of claim 1, wherein the alcohol is ethanol or methanol. 9. The formulation of claim 1, wherein the alcohol is ethanol. 10. The formulation of claim 1, wherein the alcohol is at a concentration of about 30% alcohol. 11. The formulation of claim 1, wherein the citrate is trisodium citrate. 12. The formulation of claim 1, wherein the citrate is at a concentration of about 50 mM. 13. The formulation of claim 1, wherein the formulation has a pH between about 6 and about 8. 14. The formulation of claim 1, wherein the solution has a pH between about 7.0 and about 7.5. 15. A method for denaturing protein from a solid support, comprising contacting the solid support with the formulation of claim 1, such that protein present on the solid support is denatured. 16. The method of claim 15, wherein the protein is an enzyme. 17. The method of claim 16, wherein the enzyme is a DNase. 18. The method of claim 17, wherein the DNase is DNase I. 19. The method of claim 15, wherein the solid support comprises components of silica, cellulose, cellulose acetate, nitrocellulose, nylon, polyester, polyethersulfone, polyolefin, or polyvinylidene fluoride, or combinations thereof. 20. The method of claim 15, wherein the solid support is contained in a vessel, wherein the vessel is a centrifuge tube, spin tube, syringes, cartridge, chamber, multiple-well plate, or test tube, or combinations thereof. 21. A method for purifying substantially pure and undegraded RNA from biological material comprising RNA, comprising the steps of: (a) contacting a solid support with the formulation of claim 1, such that protein present on the solid support is denatured; (b) contacting the solid support with a sample comprising RNA such that the RNA binds to the solid support; (c) washing the solid support with a series of wash solutions to remove biological materials other than bound RNA, wherein the series of wash solutions comprises a first wash containing alcohol and an RNA-complexing salt at a concentration of at least 1M and a second wash containing an alcohol, buffer and an optional chelator; and (d) preferentially eluting the bound RNA from the solid support with an RNA elution solution in order to obtain substantially pure RNA. 22. The method of claim 21, wherein the solid support is contained in a vessel, wherein the vessel is a centrifuge tube, spin tube, syringe, cartridge, chamber, multiple-well plate, test tube, or combination thereof. 23. The method of claim 21, wherein the RNA-complexing salt is lithium chloride.