Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-015/06
G01N-033/00
G01N-033/48
출원번호
US-0293214
(2002-11-13)
발명자
/ 주소
Valencia,Ramoncito Magpantay
Chen,YihFar
Hurt,Susan Newcomb
Urcia,Joseph Roby Iringan
Selvan,Gowri Pyapali
출원인 / 주소
Nagaoka & Co., Ltd.
대리인 / 주소
Knobbe, Martens, Olson & Bear, LLP
인용정보
피인용 횟수 :
33인용 특허 :
96
초록▼
The present invention is directed to optical bio-discs, methods, and optical disc drives for clinical diagnostics. The present invention is further directed to methods for determining the quantity of a specific type of blood cell in a biological sample including on-disc sample preparation and proces
The present invention is directed to optical bio-discs, methods, and optical disc drives for clinical diagnostics. The present invention is further directed to methods for determining the quantity of a specific type of blood cell in a biological sample including on-disc sample preparation and processing, providing a pre-processed sample onto a capture zone, removing portions of the sample that are not bound in the capture zone, and counting bound cells. Also disclosed are fluidic circuits for processing blood samples for use in a cluster designation marker assay in the optical bio-disc.
대표청구항▼
What is claimed is: 1. An optical bio-disc for conducting a cellular assay, said disc comprising: a rotatable substrate; a cap portion associated with said substrate; and a fluidic circuit formed between said substrate and said cap portion, said fluidic circuit comprising: a mixing chamber, said mi
What is claimed is: 1. An optical bio-disc for conducting a cellular assay, said disc comprising: a rotatable substrate; a cap portion associated with said substrate; and a fluidic circuit formed between said substrate and said cap portion, said fluidic circuit comprising: a mixing chamber, said mixing chamber including an inlet port configured to receive a fluid including RBC's; a RBC capture zone in fluid communication with the mixing chamber so as to receive a fluid therefrom; a plurality of posts with RBC capture agents coated thereon and located within the RBC capture zone, the posts being configured to capture RBC's while allowing passage through said RBC capture zone of non-RBC materials included in the fluid; a purification chamber in fluid communication with said capture zone, the purification chamber configured to receive mixed fluid communicated from the mixing chamber; a filter associated with said purification chamber; an analysis chamber in fluid communication with said purification chamber, said analysis chamber including a capture zone; and a vent port associated with said analysis chamber. 2. The optical bio-disc according to claim 1 wherein said filter is selected from the group consistingof microspheres and a cellular sieve. 3. The optical bio-disc according to claim 2 wherein said microspheres are coated with a purification agent. 4. The optical bio-disc according to claim 3 wherein said purification agent is selected from the group consisting of monoclonal antibodies, polyclonal antibodies, oligonucleotides, ligands, receptors, and binding agents. 5. The optical bio-disc according to claim 4 wherein said monoclonal antibodies are selected from the group consisting of anti-CD56, anti-CD14, anti-CD19, anti-CD9, anti-CD31, anti-CD41, anti-CD13, and anti-CD43. 6. The optical bio-disc according to claim 1 wherein said capture zone is coated with a capture agent. 7. The optical bio-disc according to claim 6 wherein said capture agent is selected from the group consisting of monoclonal antibodies, polyclonal antibodies, oligonucleotides, ligands, receptors, and binding agents. 8. The optical bio-disc according to claim 7 wherein said monoclonal antibodies are selected from the group consisting of anti-CD4, anti-CD8, and anti-CD2. 9. The optical bio-disc according to claim 1 further comprising a lysis buffer reservoir, a lysis channel connecting said lysis buffer reservoir with said mixing chamber, and a lysis buffer valve located in said lysis channel; said lysis buffer reservoir being pre-filled with a lysis buffer. 10. The optical bio-disc according to claim 9 further comprising an analysis buffer reservoir, a buffer channel connecting said analysis buffer reservoir with said purification chamber, and an analysis buffer valve located in said buffer channel; said analysis buffer reservoir being pre-filled with an analysis buffer. 11. The optical bio-disc according to claim 10 further comprising a waste chamber in fluid communication with said analysis chamber and said vent port. 12. The optical bio-disc of claim 11 further comprising a sample mix valve located in the fluidic circuit connecting said mixing chamber and said purification chamber. 13. The optical bio-disc according to claim 1 wherein said posts are distributed throughout said RBC capture zone. 14. The optical bio-disc according to claim 1 wherein said RBC capture agent is lectin. 15. A method of using the optical bio-disc according to claim 1 for a cellular assay, said method of using comprising the steps of: loading a whole blood sample into said mixing chamber through said inlet port; loading the bio-disc into an optical reader; rotating the bio-disc at a sufficient speed and time to move said sample through said RBC capture zone where red blood cells in the sample are captured, through said purification zone where unwanted cells in the sample are captured, and through said analysis chamber where specific cells are captured on the capture zone; directing an incident beam of electromagnetic radiation to the capture zone; detecting a beam of electromagnetic radiation formed after the incident beam interacts with the disc at the capture zone; converting the detected beam into an output signal; and analyzing the output signal to extract therefrom infonnation relating to the number of cells captured at the capture zone. 16. A method of making an optical bio-disc for use in cellular assays, said method of making comprising the steps of: forming a rotatable optical disc substrate; forming a cover disc with similar dimensions to said substrate; forming a channel layer with cut out portions to form fluidic circuits; said fluidic circuits including an inlet port, a mixing chamber, a purification chamber, an analysis chamber, a vent port; a RBC capture zone in fluid communication with the mixing chamber so as to receive a fluid therefrom; and a plurality of posts with RBC capture agents coated thereon and located within the RBC capture zone; said posts configured to capture RBC's while allowing passage through said RBC capture zone of non-RBC materials; and said analysis chamber including capture zones; binding capture probes onto said capture zones; attaching said channel layer to said substrate; loading a filter means into said purification chamber; attaching said cover disc to said channel layer to thereby form the optical bio-disc. 17. The method of claim 16 wherein said attaching steps are achieved by techniques selected from the group comprising adhesives and plastic welding. 18. The method of claim 16 wherein said filter means is selected from the group consisting of microspheres and cellular sieves. 19. The method of claim 18 wherein said filter means is coated with a purification agent. 20. The optical bio-disc of claim 1, wherein mixing chamber is configured to receive a lysis buffer. 21. The optical bio-disc of claim 20, wherein the bio-disc is configured to transmit the lysis buffer to the mixing chamber after the disc is rotated at a first predetermined speed, and wherein the bio-disc is configured to release the contents of the mixing chamber after the disc is rotated at a second predetermined speed.
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Sheppard ; Jr. Norman F. ; Mian Alec ; Kellogg Gregory ; Kieffer-Higgins Stephen G. ; Carvalho Bruce L., Affinity binding-based system for detecting particulates in a fluid.
Callan Gerald W. (Brewster NY) Corry William D. (Larchmont NY) Scordato Richard E. (Mount Kisco NY) Gorman John G. (New York NY), Agglutination detection apparatus.
Braynin Boris (Mountain View CA) Burd Tammy L. (Fremont CA) Ostoich Vladimir (Los Altos CA), Apparatus and method for optically analyzing biological fluids.
Kortright Kenneth H. (Cooper City FL) Coulter Wallace H. (Miami Springs FL) Rodriguez Carlos (Miami FL) Russell Thomas (Miami FL) Paul Ronald (North Miami Beach FL), Automated analyzer for screening cells or formed bodies for enumeration of populations expressing selected characteristi.
Rutenberg Mark R. (Monsey NY) Hall Thomas L. (Pasadena CA) Domes Robert (Newbury Park CA) Chaban Richard (Ventura CA) Dulak Thomas (Newhall CA) Herriman James M. (New York NY) Porzio John (New York N, Automated cytological specimen classification system and method.
Haugland Richard P. (Eugene OR) Yue Stephen T. (Eugene OR) Millard Paul J. (Eugene OR) Roth Bruce L. (Corvallis OR), Cyclic-substituted unsymmetrical cyanine dyes.
Kellogg Gregory ; Kieffer-Higgins Stephen G. ; Carvalho Bruce L. ; Davis Gene A. ; Willis John P. ; Minior Ted ; Chapman Laura L. ; Kob Mikayla ; Oeltjen Sarah D. ; Ommert Shari ; Mian Alec, Device and method for using centripetal acceleration to device fluid movement on a microfluidics system.
Greaves Geoffrey Stuart (Birmingham EN) Deverill Ian (Birmingham EN) Bunce Roger Abraham (Birmingham EN), Device for use in producing a scanning beam of radiation and apparatus for use in investigating specimens.
Mian Alec ; Kieffer-Higgins Stephen G. ; Corey George D., Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system.
Kellogg, Gregory; Kieffer-Higgins, Stephen G.; Jensen, Mona D.; Ommert, Shari; Kob, Mikayla; Pierce, Andrea; Morneau, Keith; Lin, Hsin Chiang, Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays.
Kellogg Gregory ; Kieffer-Higgins Stephen G. ; Carvalho Bruce L. ; Davis Gene A. ; Willis John P. ; Minior Ted ; Chapman Laura L. ; Kob Mikayla ; Oeltjen Sarah D. ; Ommert Shari ; Mian Alec, Devices and methods for using centripetal acceleration to drive fluid movement on a microfluidics system.
Roth Bruce L. (Corvallis OR) Millard Paul J. (Eugene OR) Yue Stephen T. (Eugene OR) Wells K. Sam (Veneta OR) Haugland Richard P. (Eugene OR), Fluorescent assay for bacterial gram reaction.
Millard Paul J. (Eugene OR) Roth Bruce L. (Corvallis OR) Yue Stephen T. (Eugene OR) Haugland Richard P. (Eugene OR), Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes.
DeCusatis Casimer M. ; Jacobowitz Lawrence, In a system for creating, reading and writing on rotatable information storage media an apparatus for determining drive.
Kelton Arden A. (Westminster CA) Waters William P. (Newport Beach CA) Shrunk David G. (Poway CA) Bell Michael L. (Hacienda Heights CA), Integrated fluid manipulator.
Ofenloch-Hhnle Beatus (Munich DEX) Berger Michael (Penzberg DEX) Deger Arno (Seeshaupt DEX), Ligand trap useful in immunoassays of biotin or free biotin containing samples and improved immunoassays using these lig.
Rothberg Jonathan Marc ; Deem Michael W. ; Simpson John W., Method and apparatus for indentifying, classifying, or quantifying protein sequences in a sample without sequencing.
Brunhouse Robert F. (Coral Gables FL) Hajek Constance M. (Miami Lakes FL) Russell Thomas (Miami FL) Coulter Wallace H. (Miami Springs FL), Method and apparatus for obtaining an absolute white blood cell subset count and white blood cell multipart differential.
Guigan Jean (9 ; rue Jean Mermoz 75008 Paris FRX), Method of performing medical analysis on a sample of liquid by means of at least one liquid reagent, and apparatus for p.
Brenner Michael B. (Sherborn MA) Der Simonian Harout (Watertown MA), Methods and compositions for detecting and treating a subset of human patients having an autoimmune disease.
Melnicoff Meryle J. (Cherry Hill NJ) Jensen Bruce D. (Schwenksville PA) Muirhead Katharine A. (West Chester PA) Horan Paul K. (Downingtown PA) Summers Martin D. (West Chester PA) Wong William (Chadsf, Methods for detection and quantification of cell subsets within subpopulations of a mixed cell population.
Bernstein Daniel M. (San Mateo CA) Lingane Paul J. (Palo Alto CA) Nagle Robert (Mountain View CA) Ostoich Vladimir E. (Los Altos CA), Methods for photometric analysis.
Zanzucchi Peter J. (Lawrenceville NJ) Cherukuri Satyam C. (Cranbury NJ) McBride Sterling E. (Lawrenceville NJ), Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis.
Zanzucchi Peter J. (West Windsor Township NJ) Cherukuri Satyam C. (Cranbury NJ) McBride Sterling E. (Lawrence Township NJ) Judd Amrit K. (Belmont CA), Partitioned microelectronic device array.
Miller Melvin N. (Wynnewood PA) Bouton John C. (Doylestown PA) Tebbe Dennis L. (West Chester PA) Leboy Eugene L. (Philadelphia PA) Anbalagan Reddiar S. (West Chester PA), Pattern recognition system for generating hematology profile.
Eppler Cecil M. (Langhorne PA) Shieh Hong-Ming (Langhorne PA) Zysk John R. (Frenchtown NJ) Corbett Martin J. (Pemberton NJ), Receptors method for purification of G protein-linked.
Burtis Carl A. (Oak Ridge TN) Johnson Wayne F. (Loudon TN) Walker William A. (Knoxville TN), Rotor assembly and method for automatically processing liquids.
Braynin Boris (Mountain View CA) Burd Tammy L. (Fremont CA) Buhl Steven (Cupertino CA) Schembri Carol T. (San Mateo CA), Sample metering port for analytical rotor having overflow chamber.
Smethers Rick T. (Milpitas CA) Leytes Lev J. (Palo Alto CA) Warner Brian D. (Martinez CA) Shadel Robert R. (San Francisco CA) Urdea Michael S. (Alamo CA), Self-contained assay assembly and apparatus.
Carrol Richard J. (Syracuse NY) Fluderer Albert A. (Marshfield MA) Smith Ward C. (Painted Post NY) Zine ; Jr. Anthony R. (Corning NY), Separation of lymphocytes and monocytes from blood samples.
Chow Calvin Y. H. (Portola Valley CA) Humphries Gillian M. (Los Altos CA) Kung Viola T. (Menlo Park CA) Lacy Michael M. (Ben Lomand CA), Single source multi-site photometric measurement system.
Fodor Stephen P. A. (Palo Alto CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Stryer Lubert (Stanford CA), Synthesis and screening of immobilized oligonucleotide arrays.
Zarling David A. ; Rossi Michel J.,CHX ; Peppers Norman A. ; Kane James ; Faris Gregory W. ; Dyer Mark J. ; Ng Steve Y. ; Schneider Luke V., Up-converting reporters for biological and other assays using laser excitation techniques.
Kim, Ji Won; Lee, Beom Seok; Kim, In Wook; Kim, Kui Hyun, Article for assaying target, comprising solid surface on which first binding member, blocking material, and second binding member are immobilized, and use thereof.
Cho, Yoon-Kyoung; Park, Ji-Woon, Centrifugal force-based microfluidic device available for reliability verification, and analyzing method using the same.
Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J., Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation.
Schaff, Ulrich Y.; Koh, Chung-Yan; Sommer, Gregory J., Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation.
McDevitt, John T.; Ballard, Karri L.; Floriano, Pierre N.; Christodoulides, Nick J.; Neikirk, Dean; Anslyn, Eric; Shear, Jason, Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems.
Koh, Chung-Yan; Light, Yooli Kim; Piccini, Matthew Ernest; Singh, Anup K., Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid.
Harding, Philip H.; Beatty, Christopher C.; Ward, Kenneth J., Method of detecting analytes in a microfluidic sample and a system for performing the same.
McDevitt, John T.; Christodoulides, Nicolaos J.; Floriano, Pierre N.; Douglas, Gary N.; Rogers, Patrick E., Methods and compositions related to determination and use of white blood cell counts.
Koh, Chung-Yan; Piccini, Matthew E.; Singh, Anup K., System and method for detecting components of a mixture including a valving scheme for competition assays.
Koh, Chung-Yan; Piccini, Matthew E.; Singh, Anup K., System and method for detecting components of a mixture including a valving scheme for competition assays.
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