[특허]Assays for detecting modulators of cytoskeletal function

[미국특허] Assays for detecting modulators of cytoskeletal function 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/53 C12N-009/14 C12N-009/16
출원번호	US-0031819 (1998-09-03)
등록번호	US-7252950 (2007-08-07)
국제출원번호	PCT/US98/018368 (1998-09-03)
§371/§102 date	20020812 (20020812)
국제공개번호	WO99/011814 (1999-03-11)
발명자 / 주소	Vale,Ronald Pierce,Daniel Spudich,James
출원인 / 주소	The Regents of the University of California The Board of Trustees of The Leland Stanford Junior University
대리인 / 주소	Medlen & Carroll, LLP
인용정보	피인용 횟수 : 1 인용 특허 : 30

초록 ▼

Described herein are methods of identifying compounds which modulate the activity of the cytoskeletal system. The methods are rapid, convenient and sensitive. Preferably, the method is used to identify lead compounds that can be used as therapeutics, diagnostics and agricultural agents. Generally, test compounds are added to two cytoskeletal components which bind to one another, to determine whether the binding is affected by the test compound. Wherein the binding is affected, a compound which modulates the cytoskeletal system is identified.

대표청구항 ▼

What is claimed is: 1. A method of identifying a therapeutic lead compound that modulates activity of a cytoskeletal system, said method comprising: i) providing an assay mixture comprising a first component of said cytoskeletal system and a second component of said cytoskeletal system, wherein said cytoskeletal system is a microtubule system, and wherein said first component comprises a kinesin motor protein and said second component comprises a tubulin protein that specifically bind to each other; ii) contacting said assay mixture with a test compound to be screened for the ability to inhibit or enhance binding between said first component and said second component; iii) detecting a change in coupling between ATP hydrolysis and force generation; wherein said change indicates that said test compound modulates activity said cytoskeletal system. 2. The method of claim 1, further comprising step iv) testing said test compound on a variety of ATPases. 3. The method of claim 1, wherein said assay mixture comprises a cell lysate. 4. The method of claim 1, wherein at least 50 test compounds are screened. 5. The method of claim 2, wherein said variety of ATPases comprises CENP-E. 6. The method of claim 2, wherein said variety of ATPases comprises kinesin. 7. The method of claim 2, wherein said variety of ATPases comprises TI-gamma.

이 특허에 인용된 특허 (30) 인용/피인용 타임라인 분석

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Goldstein Lawrence S. B. ; Faulkner David John ; Sakowicz Roman ; Berdelis Michael S. ; Blackburn Christine L. ; Hopmann Cordula,DEX, Kinesin motor modulators derived from the marine sponge Adocia.
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Gallop Mark A. (Los Altos CA) Murphy Martin A. (Mountain View CA), Methods for synthesizing diverse collections of pyrrolidine compounds.
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Holmes Christopher P. (Sunnyvale CA), Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof.
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Summerton James E. (Corvallis OR) Weller Dwight D. (Corvallis OR), Morpholino-subunit combinatorial library and method.
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Ts\o Paul O. P. (2117 Folkstone Rd. Lutherville MD 21093) Miller Paul S. (225 Hopkins Rd. Baltimore MD 21212), Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof.
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Benner Steven A. (Grossmannstrasse 16 ; #7 CH-8049 Zurich CHX), Oligonucleotide analogs containing sulfur linkages.
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Cook Phillip D. (Carlsbad CA) Kiely John (San Diego CA) Sprankle Kelly (Vista CA), Peptide nucleic acid combinatorial libraries and improved methods of synthesis.
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Ellman Jonathan A. (Berkeley CA), Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support.
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Steinert Peter M. (Rockville MD) Goldman Robert D. (Wilmette IL) DiGiovanna John J. (Bethesda MD), Use of synthetic peptides to disrupt the cytoskeleton.
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[미국특허] Assays for detecting modulators of cytoskeletal function 원문보기