IPC분류정보
| 국가/구분 |
United States(US) Patent
등록
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| 국제특허분류(IPC7판) |
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| 출원번호 |
US-0448613
(2006-06-06)
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| 등록번호 |
US-7322927
(2008-01-29)
|
|
발명자
/ 주소 |
- Buechler,Kenneth F.
- Noar,Joseph B.
- Tadesse,Lema
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| 출원인 / 주소 |
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| 대리인 / 주소 |
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| 인용정보 |
피인용 횟수 :
2 인용 특허 :
36 |
초록
▼
Water soluble hybrid phthalocyanine derivatives useful in competitive and noncompetitive assays immunoassays, nucleic acid and assays are disclosed and claimed having (1) at least one donor subunit with a desired excitation peak, and (2) at least one acceptor subunit with a desired emission peak, wh
Water soluble hybrid phthalocyanine derivatives useful in competitive and noncompetitive assays immunoassays, nucleic acid and assays are disclosed and claimed having (1) at least one donor subunit with a desired excitation peak, and (2) at least one acceptor subunit with a desired emission peak, wherein said derivative(s) is/are capable of intramolecular energy transfer from said donor subunit to said acceptor subunit. Such derivatives also may contain an electron transfer subunit. Axial ligands may be covalently bound to the metals contained in the water soluble hybrid phthalocyanine derivatives. Ligands, ligand analogues, polypeptides, proteins and nucleic acids can be linked to the axial ligands of the dyes to form dye conjugates useful in immunoassays and nucleic acid assays.
대표청구항
▼
What is claimed is: 1. A method for determining the presence or amount of an analyte of interest, said method comprising: forming a reaction mixture comprising said analyte and a population of fluorescent particles comprising receptors specific for said analyte; capturing fluorescent particles boun
What is claimed is: 1. A method for determining the presence or amount of an analyte of interest, said method comprising: forming a reaction mixture comprising said analyte and a population of fluorescent particles comprising receptors specific for said analyte; capturing fluorescent particles bound to said analyte on a solid phase; and generating a fluorescent signal from captured fluorescent particles, wherein said fluorescent particles comprise: a first compound selected from the group consisting of silicon phthalocyanine bis(dimethylhexylvinylsilyloxide) and silicon phthalocyanine bis(trihexylsilyloxide); and a second compound that is a bis(dimethylhexylvinylsilyloxide)-substituted or bis(trihexylsilyloxide)-substituted phthalocyanine, naphthalocyanine, or anthranylocyanine derivative, or a bis(dimethylhexylvinylsilyloxide)-substituted or bis(trihexylsilyloxide)-substituted hybrid phthalocyanine derivative, wherein the size of said fluorescent particle is between 0.1 nm and 5000 nm, and wherein said first compound differs in structure from said second compound. 2. The method according to claim 1, wherein said fluorescent particle comprises a latex matrix. 3. The method according to claim 1, wherein said fluorescent particle comprises a silica matrix. 4. The method according to claim 1, wherein said first compound and said second compound are each bis(dimethylhexylvinylsilyloxide)-substituted compounds. 5. The method according to claim 1, wherein said first compound is silicon phthalocyanine bis(dimethylhexylvinylsilyloxide), and said second compound is silicon 2,3-naphthalocyanine bis(dimethylhexylvinylsilyloxide). 6. The method according to claim 1, wherein said first compound is silicon phthalocyanine bis(dimethylhexylvinylsilyloxide), and said second compound is silicon phthalocyanine bis(trihexylsilyloxide). 7. The method according to claim 1, wherein said first compound is silicon phthalocyanine bis(dimethylhexylvinylsilyloxide), and said second compound is silicon [di(1,6-diphenyl-2,3-naphthalocyanine)](2,3-naphthalocyanine) phthalocyanine bis(dimethylhexylvinylsilyloxide). 8. The method according to claim 1, wherein said first compound is silicon phthalocyanine bis(dimethylhexylvinylsilyloxide), and said second compound is silicon [di(1,6-diphenyl-2,3-naphthalocyanine)][di(2,3-tert-butylphthalocyanine)] bis(dimethylhexylvinylsilyloxide). 9. The method according to claim 1, wherein said first compound is silicon phthalocyanine bis(dimethylhexylvinylsilyloxide), and said second compound is silicon [di(2,3-naphthalocyanine)][di(1,4-diphenylphthalocyanine)]bis (dimethylhexylvinylsilyloxide). 10. The method according to claim 1, wherein said first compound is silicon phthalocyanine bis(dimethylhexylvinylsilyloxide), and said second compound is silicon [di(1,6-diphenyl-2,3-naphthalocyanine)]diphthalocyanine bis(dimethylhexylvinylsilyloxide). 11. The method according to claim 1, wherein said first compound is silicon phthalocyanine bis(dimethylhexylvinylsilyloxide), and said second compound is silicon [di(1,6-diphenyl-2,3-naphthalocyanine)][di(2,3-dicyanophthalocyanine)]bis (dimethylhexylvinylsilyloxide). 12. The method according to claim 1, wherein said first compound is silicon phthalocyanine bis(dimethylhexylvinylsilyloxide), and said second compound is silicon 2,3-naphthalocyanine bis(dimethylhexylvinylsilyloxide). 13. The method according to claim 1, wherein said first compound is silicon phthalocyanine bis(dimethylhexylvinylsilyloxide), and said second compound is silicon [di(1,6-diphenylnaphthalocyanine)]diphthalocyanine bis(dimethylhexylvinylsilyloxide). 14. The method according to claim 1, wherein said receptor specific for said analyte is an antibody. 15. The method according to claim 1, wherein said receptor specific for said analyte is a nucleic acid. 16. The method according to claim 1, wherein the size of said fluorescent particle is between 1 nm and 1000 nm. 17. The method according to claim 1, wherein said signal is generated by exciting said fluorescent particles at a wavelength above 600 nm and detecting a fluorescent signal generated thereby. 18. The method according to claim 1, wherein said analyte in said reaction mixture is from a biological fluid. 19. The method according to claim 18, wherein said biological fluid is urine, blood, serum, or plasma.
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