초록 ▼

This invention relates to a method for decreasing the foam formation during cultivation of a microorganism and to a method for producing an enhanced amount of a product of interest. The method comprises that the microorganism is modified in such a way that the microorganism does not produce an essen

This invention relates to a method for decreasing the foam formation during cultivation of a microorganism and to a method for producing an enhanced amount of a product of interest. The method comprises that the microorganism is modified in such a way that the microorganism does not produce an essential amount of at least one of the proteins, polypeptides or peptides associated with foam formation during cultivation of the unmodified microorganism. In particular the method comprises that the microorganism is modified not to produce an essential amount of amphipathic or hydrophobic proteins, polypeptides or peptides.

대표청구항 ▼

The invention claimed is: 1. A method for decreasing the foam formation during cultivation of Trichoderma production host, characterized in that the process comprises the steps of genetically modifying the Trichoderma production host in such way that the Trichoderma production host produces at leas

The invention claimed is: 1. A method for decreasing the foam formation during cultivation of Trichoderma production host, characterized in that the process comprises the steps of genetically modifying the Trichoderma production host in such way that the Trichoderma production host produces at least 50% less Hydrophobin I (HFBI) or Hydrophobin II (HFBII) or both during cultivation, compared to the non-modified parent host strain thereby producing a modified Trichoderma production host; and cultivating the modified Trichoderma production host under suitable culture conditions. 2. The method of claim 1 characterized in that the genetic modification comprises genetic modification of a DNA sequence encoding the HFBI or HFBII polypeptide. 3. The method of claim 1, characterized in that the genetic modification comprises genetic modification of the regulatory region of a gene encoding the HFBI or HFBII polypeptide. 4. The method of claim 1, characterized in that the genetic modification comprises genetic modification of a DNA sequence encoding the HFBI or HFBII polypeptide. 5. The method of claim 4, characterized in that genetic modification comprises inactivation of a DNA sequence encoding the HFBI or HFBII polypeptide. 6. The method of claim 5, characterized in that the genetic modification comprises deletion of a DNA sequence encoding the HFBI or HFBII polypeptide. 7. The method of claim 1, wherein said cultivation is carried out in the presence of agitation and/or aeration. 8. The method of claim 1, wherein said cultivation occurs in a fermentor. 9. The method of claim 8, wherein said fermentor is an airlift fermentor. 10. The method of claim 1, wherein the Trichoderma production host produces 60-80% less HFBI or HFBII or both during cultivation, compared to the non-modified parent host strain. 11. The method of claim 1, wherein the Trichoderma production host produces 80-100% less HFBI or HFBII or both during cultivation, compared to the non-modified parent host strain. 12. The method of claim 1, wherein the Trichoderma production host is Trichoderma reesei. 13. The method of claim 12, wherein the Trichoderma production host is Trichoderma reesei selected from the group consisting of QM9414 (VTT-D-74075), Rut-C30 (VTT-D-86271) and QM9414 Δhfb1 (VTT-D-99724). 14. A method for decreasing the foam formation during cultivation of a Trichoderma reesei production host, characterized in that the process comprises the steps of genetically modifying the Trichoderma reesei production host in such a way that the Trichoderma reesei production host produces at least 50% less Hydrophobin I (HFBI) or Hydrophobin II (HFBII) or both during cultivation, compared to the non-modified parent host strain thereby producing a modified Trichoderma reesei production host; and cultivating the modified Trichoderma reesei production host under suitable culture conditions in the presence of agitation and/or aeration in an airlift fermentor. 15. The method according to claim 14, wherein the Trichoderma reesei production host is Trichoderma reesei selected from the group consisting of QM9414 (VTT-D-74075), Rut-C30 (VTT-D-86271) and QM9414 Δhfb1 (VTT-D-99724). 16. The method according to claim 14, wherein the Trichoderma reesei production host produces 80-100% less HFBI or HFBII or both during cultivation, compared to the non-modified parent host strain. 17. The method according to claim 14, wherein the cultivation is performed in the absence of antifoaming agent.