Methods for treating mastitis in a milk producing animal
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-039/108
A61K-039/00
A61K-039/38
A61K-039/116
A61K-038/00
A61K-039/112
A01N-037/18
출원번호
US-0829838
(2004-04-22)
등록번호
US-7341732
(2008-03-11)
발명자
/ 주소
Emery,Daryll A.
Straub,Darren E.
출원인 / 주소
Epitopix, LLC
대리인 / 주소
Mueting, Raasch & Gebhardt, P.A.
인용정보
피인용 횟수 :
22인용 특허 :
33
초록▼
The present invention provides methods for treating mastitis in a milk producing animal. The methods include administering compositions including siderophore receptor polypeptides and porins from gram negative microbes, and preferably, lipopolysaccarhide at a concentration of no greater than about 1
The present invention provides methods for treating mastitis in a milk producing animal. The methods include administering compositions including siderophore receptor polypeptides and porins from gram negative microbes, and preferably, lipopolysaccarhide at a concentration of no greater than about 10.0 endotoxin units per milliliter.
대표청구항▼
What is claimed is: 1. A method for treating mastitis in a milk producing animal, the method comprising administering to a milk producing animal having or at risk of having mastitis an effective amount of a composition comprising: at least two siderophore receptor polypeptides isolated from a gram
What is claimed is: 1. A method for treating mastitis in a milk producing animal, the method comprising administering to a milk producing animal having or at risk of having mastitis an effective amount of a composition comprising: at least two siderophore receptor polypeptides isolated from a gram negative microbe; at least two porins isolated from the gram negative microbe; lipopolysaccarhide at a concentration of no greater than about 10.0 EU/ml; and a pharmaceutically acceptable carrier. 2. The method of claim 1 wherein the gram negative microbe is an enteropathogen. 3. The method of claim 1 wherein the gram negative microbe is a member of the family Enterobacteriaceae. 4. The method of claim 1 wherein the gram negative microbe is a member of the tribe Escherichieae or Salmonelleae. 5. The method of claim 1 wherein the gram negative microbe is Salmonella spp. or Escherichia coli. 6. The method of claim 1 wherein the at least two siderophore receptor polypeptides have molecular weights of between about 60 kDa and about 100 kDa as determined by separation by sodium dodecyl-polyacrylamide gel electrophoresis. 7. The method of claim 1 wherein the at least two porins have molecular weights of between about 30 kDa and about 43 kDa as determined by separation by sodium dodecyl-polyacrylamide gel electrophoresis. 8. The method of claim 1 wherein the animal is a bovine. 9. The method of claim 1 wherein the mastitis is caused by a gram negative microbial infection. 10. A method for treating mastitis in a milk producing animal, the method comprising administering to a milk producing animal having or at risk of having mastitis an effective amount of a first composition comprising: at least two siderophore receptor polypeptides isolated from a gram negative microbe; at least two porins isolated from the gram negative microbe; lipopolysaccarhide; and a pharmaceutically acceptable carrier, wherein the concentration of lipopolysaccharide present in the first composition is no greater than the concentration of lipopolysaccharide in a second composition comprising: the at least two siderophore receptor polypeptides isolated from the gram negative microbe; the at least two porins isolated from the gram negative microbe; lipopolysaccarhide; wherein the second composition is produced by a process comprising: providing the gram negative microbe; disrupting the gram negative microbe in a buffer; solubilizing the disrupted gram negative microbe for greater than about 24 hours in a solution comprising sarcosine to result in solubilized and insoluble cellular material, wherein a ratio of the sarcosine to gram weight of disrupted gram negative microbe is between about 0.8 gram sarcosine per about 4.5 grams of disrupted gram negative microbe and about 1.2 grams sarcosine per about 4.5 grams of disrupted gram negative microbe; and isolating molecules of the gram negative microbe, wherein the isolated molecules comprise the at least two siderophore receptor polypeptides, the at least two porins, and lipopolysaccarhide. 11. The method of claim 10 wherein the gram negative microbe is an enteropathogen. 12. The method of claim 10 wherein the gram negative microbe is a member of the family Enterobacteriaceae. 13. The method of claim 10 wherein the gram negative microbe is a member of the tribe Escherichieae or Salmonelleae. 14. The method of claim 10 wherein the gram negative microbe is Salmonella spp. or Eseherichia coli. 15. The method of claim 10 wherein the at least two siderophore receptor polypeptides have molecular weights of between about 60 kDa and about 100 kDa as determined by separation by sodium dodecyl-polyacrylamide gel electrophoresis. 16. The method of claim 10 wherein the at least two porins have molecular weights of between about 30 kDa and about 43 kDa as determined by separation by sodium dodecyl-polyacrylamide gel electrophoresis. 17. The method of claim 10 wherein the animal is a bovine. 18. The method of claim 10 wherein the mastitis is caused by a gram negative microbial infection. 19. The method of claim 9 wherein the gram negative microbial infection is E. coli, Enterobacter aerogenes, Aerobacter spp., Bacteroides spp., Campylobacter spp., Citrobacter spp., Enterobacter spp., Erwinia spp., Escherichia spp., Fusobacaterium spp., Kiebsiella spp., Leptospira spp., Mycoplasma spp., Pasteurella spp., Providencia spp., Pseudomonas spp., Proteus spp., Serratia spp., Salmonella spp., or Yersinia spp. 20. The method of claim 18 wherein the gram negative microbial infection is E. coli, Enterobacter aerogenes, Aerobacter spp., Bacteroides spp., Campylobacter spp., Citrobacter spp., Enterobacter spp., Erwinia spp., Eseherichia spp., Fusobacaterium spp., Kiebsiella spp., Leptospira spp., Mycoplasma spp., Pasteurella spp., Providencia spp., Pseudomonas spp., Proteus spp., Serratia spp., Salmonella spp., or Yersinia spp. 21. The method of claim 10 wherein the LPS concentration of the first composition and the second composition is no greater than about 10.0 EU/ml. 22. The method of claim 10 wherein the providing comprises incubating the gram negative microbe in media comprising an iron chelator. 23. The method of claim 22 wherein the iron chelator is 2,2'-dipyridyl.
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이 특허에 인용된 특허 (33)
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Emery,Daryll A.; Straub,Darren E.; Zammert,Donavan E.; Kallevig,Gayla K., Methods for making compositions including gram negative microbial polypeptides.
Cochrum Kent C. (Davis CA) Dorian Randel E. (Orinda CA) Jemtrud Susan A. (San Francisco CA), Multiple layer alginate coatings of biological tissue for transplantation.
Tam, Patricia E.; Harris, Michael Nathan; Tripp, Timothy James; Lobo, Sandra, Polypeptides and immunizing compositions containing Bacillus polypeptides and methods of use.
Emery, Daryll A.; Straub, Darren E.; Herron-Olson, Lisa L.; Wonderling, Laura, Polypeptides and immunizing compositions containing gram positive polypeptides and methods of use.
Emery, Daryll A.; Straub, Darren E.; Herron-Olson, Lisa L.; Wonderling, Laura, Polypeptides and immunizing compositions containing gram positive polypeptides and methods of use.
Emery, Daryll A.; Straub, Darren E.; Herron-Olson, Lisa L.; Wonderling, Laura, Polypeptides and immunizing compositions containing gram positive polypeptides and methods of use.
Emery, Daryll A.; Straub, Darren E.; Wonderling, Laura; Herron Olson, Lisa L., Polypeptides and immunizing compositions containing gram positive polypeptides and methods of use.
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