IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0864219
(2004-06-09)
|
등록번호 |
US-7381559
(2008-06-03)
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발명자
/ 주소 |
- Ellis,Samuel A.
- Harlan,Jeffrey L.
|
출원인 / 주소 |
- Scientific Plastic Products, Inc.
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
9 인용 특허 :
15 |
초록
▼
A container is provided for cell growth by artificial cultivation of selected biological material in a growth liquid for use in an oscillating incubator. The container has a circular bottom with rounded corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the contain
A container is provided for cell growth by artificial cultivation of selected biological material in a growth liquid for use in an oscillating incubator. The container has a circular bottom with rounded corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the container to define a container volume. The bottom has six equally spaced baffles extending inward from the corners and upward toward a large diameter opening of the container centered on the longitudinal axis. The baffles have a triangular cross-sectional shape with an included angle of about 28-40��, and a height measured parallel to the longitudinal axis of about 15-25% of the usable container volume. The baffles end before the longitudinal axis. The container and baffles are blow molded of a polymer suitable for blow molding. An air permeable filter is placed over the container opening, with the filter having an adhesive on one side to stick to the container. A finger tab on the periphery of the filter helps position and remove the filter.
대표청구항
▼
What is claimed is: 1. A container for cell growth by artificial cultivation of selected biological material in a growth liquid for use in an oscillating incubator, comprising: the container having a circular bottom with rounded corners joining a sidewall that is inclined at an angle toward a longi
What is claimed is: 1. A container for cell growth by artificial cultivation of selected biological material in a growth liquid for use in an oscillating incubator, comprising: the container having a circular bottom with rounded corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the container to define a usable container volume, the bottom having a diameter with six equally spaced baffles extending inward from the corners toward the longitudinal axis and upward toward a large diameter opening of the container centered on the longitudinal axis, the baffles having a triangular cross-sectional shape with an apex having an included angle of about 28-40��, and a height measured parallel to the longitudinal axis of about 15-25% of the usable container volume, the baffles ending before the longitudinal axis but extending to within a distance from that axis that is at least about 1/6 the diameter of the bottom, the container and baffles being blow molded of a polymer suitable for blow molding. 2. The container of claim 1, wherein the baffles extend radially inward and end before the longitudinal axis at a distance of about 1/6 to 1/7 the diameter of the bottom. 3. The container of claim 1, further comprising a pipette port formed in the sidewall. 4. The container of claim 3, wherein the port is within about six inches or less of the intersection of the longitudinal axis and the bottom and sized to allow the insertion of a pipette. 5. The container of claim 3, further comprising a filter over the port. 6. The container of claim 1, further comprising a flat filter over the opening. 7. The container of claim 1, further comprising a flat filter over the opening, the filter having an adhesive surface thereon adhered to the container. 8. The container of claim 7, further comprising a finger tab extending from a periphery of the filter. 9. The container of claim 7, further comprising a plurality of slits extending inward from the periphery of the filter. 10. The container of claim 1, wherein the baffles have an included angle of 34��. 11. The container of claim 1, wherein the baffles have an apex that curves toward the bottom in approximation to a quadrant of an ellipse where the ellipse is inclined at about 8-15��. 12. The container of claim 1, wherein the baffles have a maximum height of about 3/4 inch. 13. The container of claim 1, wherein the bottom is curved upward slightly from the corners to the intersection of the bottom with the longitudinal axis. 14. The container of claim 1, wherein a biological material and growth liquid are placed in the container to a level that is about 0.8 to 1.25 times the height of the baffles measured at the corners of the container. 15. The container of claim 1, wherein the one side of each baffle is inclined and the opposing side is substantially perpendicular. 16. The container of claim 1, wherein at least one of the baffles has a side that is concave. 17. The container of claim 1, wherein the bottom comprises a sheet of material that is deformed to define the shape of the baffle. 18. A kit for cell growth by artificial cultivation of selected biological material in a growth liquid for use in an oscillating incubator, the kit comprising: a container having a large opening centered on a longitudinal axis, the container having a circular bottom with rounded corners joining a sidewall enclosing a usable volume, the bottom having six equally spaced baffles extending inward from the corners toward a central, longitudinal axis, the baffles extending upward toward a large diameter opening of the container that is centered on the longitudinal axis and connected to the sidewall, the baffles having a triangular cross-sectional shape with an included angle of about 28-40��, the baffles ending before the longitudinal axis and having the sides of the baffles join at an apex that curves or approximates a curve, the container and baffles being blow molded of a polymer suitable for blow molding; and a first air permeable filter sized to cover the large opening of the container. 19. The kit of claim 18, wherein the container is placed in a bag, sealed and sterilized. 20. The kit of claim 18, wherein the container and filter are each placed in a same bag, sealed and sterilized. 21. The kit of claim 18, wherein the container is placed in a first bag, sealed and sterilized and the filter is placed in a second bag, sealed and sterilized. 22. The kit of claim 18, wherein the container has a pipette access port in the sidewall, and the kit further comprises a second filter sized to cover the access port. 23. The kit of claim 18, wherein the baffles have an included angle of about 34�� or less and end before the longitudinal axis a distance of about 1/6 to about 1/7 the diameter of the bottom. 24. The kit of claim 18, wherein the filter has a finger tab extending from a periphery of the filter. 25. The kit of claim 18, wherein the baffles have a height of about one inch or less and wherein the baffles curve toward the bottom in approximation to a quadrant of an ellipse where the ellipse is inclined at about 8-15�� and end before the longitudinal axis at a distance of about 1/6 to 1/7 the diameter of the bottom. 26. The kit of claim 18, wherein each baffle has a leading side and an opposing side each of which is inclined about the same angle. 27. The kit of claim 18, wherein each baffle has a leading side and an opposing side one of which is substantially vertical and the other of which is inclined at an angle of about 17��. 28. The kit of claim 18, wherein the filter comprises an air permeable tape with releasable adhesive on one side of the tape. 29. A container for cell growth by artificial cultivation of selected biological material in a growth liquid for use in an oscillating incubator, comprising: the container having a large opening centered on a longitudinal axis, the container having a circular bottom with rounded corners joining a sidewall enclosing a usable volume, the bottom having four to eight equally spaced baffles extending inward from the corners toward a central, longitudinal axis and ending before that axis at a distance of about 1/6 to 1/7 the diameter of the bottom, the baffles extending upward toward a large diameter opening of the container that is centered on the longitudinal axis and connected to the sidewall, the baffles having a triangular cross-sectional shape with a leading and trailing side each upwardly inclined and intersecting at an apex having an included angle, θ, the baffles ending before the longitudinal axis and having apex curve or approximates a curve, the container and baffles being blow molded of a polymer suitable for blow molding; and a first air permeable filter sized to cover the large opening of the container. 30. The container of claim 29, wherein the included angle θ is about 28-40��. 31. The container of claim 29, wherein the included angle θ is about 17��. 32. The container of claim 29, wherein one of the sides of a plurality of the baffles is inclined and the other side of that plurality is substantially vertical. 33. The container of claim 29, further comprising a pipette access port formed in the sidewall. 34. The container of claim 29, further comprising a pipette access port formed in the sidewall and a second filter covering that access port. 35. The container of claim 29, wherein the baffles have a height of about 0.5 inches to about one inch. 36. The container of claim 29, wherein the filter comprises a flat disk with releasable adhesive on one side of the filter. 37. The container of claim 29, wherein the filter comprises an air permeable adhesive tape. 38. A container for cell growth by artificial cultivation of selected biological material in a growth liquid, the bottle being configured for use in an oscillating incubator, comprising: the cell growth container having a circular bottom with rounded corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the container to define a usable container volume, the bottom having a diameter and having a plurality of spaced baffles extending inward from the corners toward the longitudinal axis and upward toward a large diameter opening of the container centered on the longitudinal axis, the baffles extending from the corners to within a distance of that axis that is at least 1/6 the diameter of the bottom, the plurality of baffles having first and second sides, with at least one of the first and second sides being inclined relative to a plane in which the longitudinal axis lies and inclined in an orientation to allow fluid in the flask to flow up and over the incline during oscillation, wherein the baffles end before the longitudinal axis and have distal ends that extend away from the opening to join a central portion of the bottom centered on the longitudinal axis, the central portion of the bottom being further from the opening than the adjacent ends of the baffles. 39. The container of claim 38, wherein the baffles having a height measured parallel to the longitudinal axis of about 15-25% of the usable container volume, the baffles ending before the longitudinal axis, the container and baffles being blow molded of a polymer suitable for blow molding. 40. The container of claim 38, wherein the baffles extend radially inward. 41. The container of claim 38, wherein the first face is inclined and the second face is perpendicular to the bottom. 42. The container of claim 38, wherein both the first and second faces are inclined. 43. A container for cell growth by artificial cultivation of selected biological material in a growth liquid, the bottle being configured for use in an oscillating incubator, comprising: the cell growth container having a circular bottom with rounded corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the container to define a usable container volume, the bottom having a diameter with a plurality of spaced baffles extending inward from the corner toward the longitudinal axis and upward toward a large diameter opening of the container centered on the longitudinal axis, the baffles extending from the corners to within a distance of that axis that is at least 1/6 the diameter of the bottom, the plurality of baffles having first and second sides, with at least one of the first and second sides being inclined relative to a plane in which the longitudinal axis lies and inclined in an orientation to allow fluid in the flask to flow up and over the incline during oscillation, wherein the baffles have a height measured parallel to the longitudinal axis of about 15-25% of the usable container volume, the baffles ending before the longitudinal axis, the container and baffles being blow molded of a polymer suitable for blow molding. 44. A container for cell growth by artificial cultivation of selected biological material in a growth liquid for use in an oscillating incubator, comprising: a circular bottom on the cell growth container, the bottom having corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the container to define a container volume, the bottom having baffle means for agitating the biological material and liquid during use, wherein interior ends of the baffle means end at a central portion of the bottom that is lower than the adjacent ends of the baffle means and that is centered on the longitudinal axis. 45. A container for cell growth by artificial cultivation of selected biological material in a growth liquid for use in an oscillating incubator, comprising: a circular bottom on the cell growth container, the bottom having corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the container to define a container volume, the bottom having a diameter and having baffle means for agitating the biological material and liquid during use, wherein interior ends of the baffle means and that is centered on the longitudinal axis, the baffles means extending from corners of the container to within a distance of that axis that is at least 1/6 the diameter of the bottom. 46. The container of claim 1, wherein the baffles have a length and an apex that is not straight for its entire length. 47. The container of claim 1, wherein the baffles have a length and height, and a plurality of the baffles have a height of about .5 to about 1 inches for more than half the length of the baffle. 48. The container of claim 1, wherein the baffles have a length and a plurality of the baffles have an apex which inclines at an angle to the horizontal of about 11 degrees below horizontal.
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