IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0139145
(2005-05-27)
|
등록번호 |
US-7442538
(2008-10-28)
|
우선권정보 |
ES-200202828(2002-11-28) |
발명자
/ 주소 |
- Bragos Bardia,Ramon
- Rosell Ferrer,Javier
- Riu Costa,Pere Joan
- Godia Casablancas,Francesc
- Cairo Badillo,Jordi Joan
- Paredes Mu��oz,Carles
- Rodriguez Omedes,Ferran
|
출원인 / 주소 |
- Hexascreen Culture Technologies, S.L.
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
3 인용 특허 :
4 |
초록
▼
The invention relates to a method and a device which are used to obtain and maintain multiple biological cultures, with environmental parameters similar to those of larger bioreactors (from 1 L) and with a degree of measurement automation similar to that currently obtained using microplates (1-2 mL)
The invention relates to a method and a device which are used to obtain and maintain multiple biological cultures, with environmental parameters similar to those of larger bioreactors (from 1 L) and with a degree of measurement automation similar to that currently obtained using microplates (1-2 mL). The aforementioned environmental parameters include at least the following: measurement and control of temperature, agitation and aeration, measurement and control of pH and dissolved oxygen and measurement of the biomass.
대표청구항
▼
The invention claimed is: 1. A device for substantially simultaneously and automatically carrying out a plurality of biological cultures at small volumes and with controlled conditions similar to those which can be obtained with large scale bioreactors, the device comprising: at least one plate whi
The invention claimed is: 1. A device for substantially simultaneously and automatically carrying out a plurality of biological cultures at small volumes and with controlled conditions similar to those which can be obtained with large scale bioreactors, the device comprising: at least one plate which comprises a plurality of minibioreactors for culturing in sterile conditions, each minibioreactor being sealed to the environment, to the rest of the minibioreactors and to a common thermostat bath, each minibioreactor including an individual stirring member for providing homogenization of any contents of the minibioreactor, and one or more sterile access points enabling one or more of filling, inoculation, gas exchange, liquid exchange and parameter measurement; and a support device intended for receiving the at least one plate therein, each of the at least one plates being fitted through the opening of at least one part of said support device, said support device comprising one or more means for controlled heat exchange with the thermostat bath, means for energy transmission to the individual stirring members, means for gas exchange through sterile filters and means for non-invasive monitoring and/or controlling of the culture parameters, said means for gas exchange and said means for monitoring and/or controlling the culture parameters each comprising individual probes and individual connections for each minibioreactor, which can be simultaneously engaged with said sterile access points of the minibioreactor. 2. A device as in claim 1, wherein each of the at least one plates further comprises a thermostat bath central stirrer. 3. A device as in claim 1, wherein the at least one plate comprises a number of individual minibioreactors in a polygonal arrangement, with a central area which facilitates common thermostating of said minibioreactors. 4. A device as in claim 1, wherein the device is connected to a computer system, either via a dedicated connection or through a data communication network. 5. A device as in claim 1, wherein each minibioreactor comprises an upper cover having an optical port including a tube that penetrates within the minibioreactor so that its lower end is immersed into a liquid contained in said minibioreactor. 6. A device as in claim 5, wherein an optical sensor and light source is placed in the optical port, at the bottom portion of the plate facing said optical port and/or on the lateral surface of the reservoir. 7. A device as in claim 5, wherein an optical fiber or light guide is placed in the optical port, at the bottom portion of the plate facing said optical port and/or on the lateral surface of the reservoir. 8. A device as in claim 5, wherein an additional port is fitted through the upper cover so that the end of said port is immersed into the liquid, said end being provided with a filter or semi-permeable membrane. 9. A device as in claim 5, wherein an additional port is fitted through the upper cover so that the end of said port is immersed into the liquid, said end containing a sensor or microsensor. 10. A device as in claim 1, wherein heat exchange between the support device and the thermostat bath of the plate is carried out by one or more of by means of a heating resistance that is in contact with a portion of the plate outer surface; by means of a Peltier cell that is in contact with a portion of the plate outer surface; by means of a heating resistance that is immersed into said bath; and by radio frequency heating performed from the outside. 11. A device as in claim 1 further comprising a temperature probe immersed into one of the thermostat bath and the contents of one or more of the minibioreactors. 12. A device as in claim 1 further comprising an additional minibioreactor surrounded by the thermostat bath, in which a temperature probe is immersed. 13. A device as in claim 1 further comprising a stator located below each individual stirring member, for energy transmission to each said stirring member. 14. A device as in claim 1 further comprising a rotating magnet located below one or more of the individual stirring members, for energy transmission to each respective stirring member, each said magnet being rotated by one of a mechanical driving system actuated by a common electric motor and a respective individual electric motor. 15. A device as in claim 2 further comprising a stator or a rotating magnet located below the thermostat bath central stirrer, for energy transmission to said stirrer. 16. A device as in claim 2 further comprising means for causing the common thermostat bath stirrer to be rotated from a drive providing controlled sequencing of the minibioreactors. 17. A device as in claim 1 wherein each minibioreactor has a volume ranging from 5 to 25 ml. 18. A device as in claim 1 wherein the plate is at least in part disposable. 19. A device as in claim 1 wherein the plate is formed of a base made of plastic material and at least one cover made of plastic material or other transparent material. 20. A method for substantially simultaneously and automatically carrying out a number of biological cultures at small volumes and with controlled conditions similar to those which can be obtained with large scale bioreactors, the method comprising: carrying out cultures in sterile conditions in a number of minibioreactors provided in a plate, each of said minibioreactors being sealed to the environment, to the rest of the minibioreactors and to a common thermostat bath, and each minibioreactor including an individual stirring member for allowing homogenization of its contents, and one or more sterile access points enabling one or more of filling, inoculation, gas exchange, liquid exchange and parameter measurement; fitting at least one plate into a support device which comprises one or more of means for controlled heat exchange with the thermostat bath, means for energy transmission to the individual stirring members, means for gas exchange through sterile filters and means for non-invasive monitoring and/or controlling of the culture parameters, said means for gas exchange and for monitoring or controlling of the culture parameters comprising individual probes and connections for each minibioreactor, which can be simultaneously engaged with said sterile access points of the minibioreactor; and non-invasive monitoring and/or controlling of the culture parameters.
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