[특허]In situ heat induced antigen recovery and staining apparatus and method

[미국특허] In situ heat induced antigen recovery and staining apparatus and method 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	B01L-003/02 G01N-021/01
출원번호	US-0943546 (2004-09-17)
등록번호	US-7476362 (2009-01-13)
발명자 / 주소	Angros,Lee
출원인 / 주소	Angros,Lee
대리인 / 주소	Dunlap Codding, P.C.
인용정보	피인용 횟수 : 58 인용 특허 : 71

초록 ▼

An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide. The reagents are preferably held in a reagent dispensing strip similar to a "blister pack".

대표청구항 ▼

What is claimed is: 1. An apparatus for treating microscope slides, comprising: a slide support assembly comprising a first slide support element for supporting a single first microscope slide and at least one second slide support element for supporting a single second microscope slide, wherein the first slide support element and the at least one second slide support element are each independently movable relative to each other and wherein each first slide support element and each at least one second slide support element is movable by tilting; a first heating element in the first slide support element for heating the first microscope slide and a second heating element in the at least one second slide support element for heating the second microscope slide and wherein the first heating element can heat the first microscope slide independently of the heating of the second microscope slide by the second heating element; a treatment chamber able to contain the first slide support element having the first microscope slide disposed thereon and able to contain the at least one second slide support element having the second microscope slide disposed thereon; a first reagent dispenser for dispensing a first reagent to the first microscope slide from a position above the first microscope slide disposed on the first slide support element and a second reagent dispenser for independently dispensing a second reagent to the second microscope slide disposed on the at least one second slide support element from a position above the second microscope slide; and electromechanical means for controlling application of a first treatment protocol to the first microscope slide and application of a second treatment protocol to the second microscope slide and for controlling movement of the first slide support element into or out of the treatment chamber while the second treatment protocol is being applied to the second microscope slide within the treatment chamber and for controlling movement of the at least one second slide support element into and out of the treatment chamber while the first treatment protocol is being applied to the first microscope slide within the treatment chamber. 2. The apparatus of claim 1 wherein the electromechanical means comprises a microprocessor. 3. The apparatus of claim 1 wherein the treatment chamber comprises a first reaction compartment for containing the first slide support element and at least one second reaction compartment for containing the at least one second slide support element. 4. The apparatus of claim 1 wherein the first reagent dispenser dispenses at least one reagent which corresponds only to the first microscope slide on the first slide support element. 5. The apparatus of claim 1 wherein the second reagent dispenser dispenses at least one reagent which corresponds only to the second microscope slide on the at least one second slide support element. 6. The apparatus of claim 1 wherein the first treatment protocol can be completely applied to the first microscope slide simultaneously and independently of complete application of the second treatment protocol to the second microscope slide. 7. The apparatus of claim 1 further comprising a first reagent dispenser holder for holding a first reagent pack and for dispensing a reagent therefrom and a second reagent dispenser holder for holding a second reagent pack and for dispensing a reagent therefrom. 8. The apparatus of claim 7 wherein the first reagent pack on the first reagent dispenser holder and second reagent pack on the second reagent dispenser holder are operable and movable independently of each other and/or simultaneously with each other. 9. The apparatus of claim 7 comprising a first pressing mechanism for dispensing the reagent from the first reagent pack and a second pressing mechanism for dispensing the reagent from the second reagent pack, and wherein each first and second pressing mechanism is able to function independently of each other and/or simultaneously with each other. 10. The apparatus of claim 3 wherein the first reaction compartment and the at least one second reaction compartment can each be independently enclosed. 11. The apparatus of claim 10 wherein each first reaction compartment and each at least one second reaction compartment can be vented to release pressure, steam or vapors from an inner space thereof during heating of the microscope slide within the first reaction compartment or the at least one second reaction compartment. 12. The apparatus of claim 11 wherein the first reaction compartment and the at least one second reaction compartment each comprises a device to independently release vapor pressure. 13. An apparatus for treating microscope slides, comprising: a slide support assembly comprising a plurality of slide support elements each for sporting a single microscope slide thereon wherein each of the slide support elements is movable independently of each other and wherein each slide support element is movable by tilting; a heating element in each of the slide support elements for heating the microscope slide disposed upon each of the support elements wherein each microscope slide can be heated independently; a treatment chamber able to contain each of the plurality of slide support elements having the microscope slides disposed thereon; a plurality of reagent dispensers for dispensing reagents to the microscope slides from positions above the microscope slides disposed on the slide support elements; and electromechanical means for controlling application of a separate treatment protocol to each microscope slide disposed on each slide support element and for controlling movement of at least one slide support element into or out of the treatment chamber while at least one of the other microscope slides upon a corresponding slide support element is being treated with a corresponding treatment protocol within the treatment chamber. 14. The apparatus of claim 13 wherein the electromechanical means comprises a microprocessor. 15. The apparatus of claim 13 wherein the treatment chamber comprises a plurality of reaction compartments for containing the plurality of slide support elements. 16. The apparatus of claim 13 wherein each reagent dispenser dispenses at least one reagent which corresponds only to a particular microscope slide on a particular slide support element. 17. The apparatus of claim 13 wherein each treatment protocol can be completely applied to the microscope slide simultaneously and independently of complete application of the each other treatment protocol to the each other microscope slide. 18. The apparatus of claim 13 further comprising a plurality reagent dispenser holders for holding a reagent pack and for dispensing a reagent therefrom. 19. The apparatus of claim 18 wherein the each reagent pack on the each reagent dispenser holder is operable and movable independently of each other and/or simultaneously with each other. 20. The apparatus of claim 18 comprising a plurality of pressing mechanisms for dispensing the reagent from each corresponding reagent packs and wherein each pressing mechanism is able to function independently of each other pressing mechanism. 21. The apparatus of claim 14 wherein each reaction compartment is independently encloseable. 22. The apparatus of claim 21 wherein each reaction compartment can be vented to release pressure, steam or vapors from an inner space thereof during heating of the microscope slide within the reaction compartment. 23. The apparatus of claim 22 wherein each reaction compartment comprises a device to independently release vapor pressure. 24. An apparatus for treating microscope slides, comprising: a slide support assembly comprising a first slide support element for supporting a single first microscope slide and at least one second slide support element for supporting a single second microscope slide, wherein the first slide support element and the at least one second slide support element are each independently movable relative to each other and wherein each first slide support element and each at least one second slide support element is movable horizontally; a first heating element in the first slide support element for heating the first microscope slide and a second heating element in the at least one second slide support element for heating the second microscope slide and wherein the first heating element can heat the first microscope slide independently of the heating of the second microscope slide by the second heating element; a treatment chamber able to contain the first slide support element having the first microscope slide disposed thereon and able to contain the at least one second slide support element having the second microscope slide disposed thereon; a first reagent dispenser for dispensing a first reagent to the first microscope slide from a position above the first microscope slide disposed on the first slide support element and a second reagent dispenser for independently dispensing a second reagent to the second microscope slide disposed on the at least one second slide support element from a position above the second microscope slide; and electromechanical means for controlling application of a first treatment protocol to the first microscope slide and application of a second treatment protocol to the second microscope slide, and for controlling movement of the first slide support element into or out of the treatment chamber while the second treatment protocol is being applied to the second microscope slide within the treatment chamber, and for controlling movement of the at least one second slide support element into or out of the treatment chamber while the first treatment protocol is being applied to the first microscope slide within the treatment chamber. 25. The apparatus of claim 24 wherein the electromechanical means comprises a microprocessor. 26. An apparatus for treating microscope slides, comprising: a slide support assembly comprising a plurality of slide support elements each for supporting a single microscope slide thereon wherein each of the slide support elements is movable independently of each other and wherein each slide support element is movable horizontally; a heating element in each of the slide support elements for heating the microscope slide disposed upon each of the support elements wherein each microscope slide can be heated independently; a treatment chamber able to contain each of the plurality of slide support elements having the microscope slides disposed thereon; a plurality of reagent dispensers for dispensing reagents to the microscope slides from positions above the microscope slides disposed on the slide support elements; and electromechanical means for controlling application of a separate treatment protocol to each microscope slide disposed on each slide support element and for controlling movement of at least one slide support element into or out of the treatment chamber while at least one of the other microscope slides upon a corresponding slide support element is being treated with a corresponding treatment protocol within the treatment chamber. 27. The apparatus of claim 26 wherein the electromechanical means comprises a microprocessor. 28. An apparatus for treating microscope slides, comprising: a slide support assembly comprising a first slide support element for supporting a single first microscope slide and at least one second slide support element for supporting a single second microscope slide, wherein the first slide support element and the at least one second slide support element are each independently movable relative to each other and wherein each first slide support element and each at least one second slide support element is movable upwardly and downwardly; a first heating element in the first slide support element for heating the first microscope slide and a second heating element in the at least one second slide support element for heating the second microscope slide and wherein the first heating element can heat the first microscope slide independently of the heating of the second microscope slide by the second heating element; a treatment chamber able to contain the first slide support element having the first microscope slide disposed thereon and able to contain the at least one second slide support element having the second microscope slide disposed thereon; a first reagent dispenser mechanism for dispensing a first reagent to the first microscope slide from a position above the first microscope slide disposed on the first slide support element and a second reagent dispenser for independently dispensing a second reagent to the second microscope slide disposed on the at least one second slide support element from a position above the second microscope slide; and electromechanical means for controlling application of a first treatment protocol to the first microscope slide and application of a second treatment protocol to the second microscope slide, and for controlling movement of the first slide support element into or out of the treatment chamber while the second treatment protocol is being applied to the second microscope slide within the treatment chamber, and for controlling movement of the at least one second slide support element into or out of the treatment chamber while the first treatment protocol is being applied to the first microscope slide within the treatment chamber. 29. The apparatus of claim 28 wherein the electromechanical means comprises a microprocessor. 30. An apparatus for treating microscope slides, comprising: a slide support assembly comprising a plurality of slide support elements each for supporting a single microscope slide thereon wherein each of the slide support elements is movable independently of each other and wherein each slide support element is movable upwardly and downwardly; a heating element in each of the slide support elements for heating the microscope slide disposed upon each of the support elements wherein each microscope slide can be heated independently; a treatment chamber able to contain each of the plurality of slide support elements having the microscope slides disposed thereon; a plurality of reagent dispensers for dispensing reagents to the microscope slides from positions above the microscope slides disposed on the slide support elements; and electromechanical means for controlling application of a separate treatment protocol to each microscope slide disposed on each slide support element and for controlling movement of at least one slide support element into or out of the treatment chamber while at least one of the other microscope slides upon a corresponding slide support element is being treated with a corresponding treatment protocol within the treatment chamber. 31. The apparatus of claim 30 wherein the electromechanical means comprises a microprocessor.

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Angros, Lee, In situ heat induced antigen recovery and staining apparatus and method.
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Merrow, Brian S.; Akers, Larry W., Storage device testing system with a conductive heating assembly.
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Merrow, Brian S., Temperature control within disk drive testing systems.
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Merrow, Brian S., Temperature control within disk drive testing systems.
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Merrow, Brian S., Temperature control within storage device testing systems.
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Merrow, Brian S.; Krikorian, Nicholas C., Test slot cooling system for a storage device testing system.
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Merrow, Brian S.; Krikorian, Nicholas C., Test slot cooling system for a storage device testing system.
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Merrow, Brian S., Thermal control system for test slot of test rack for disk drive testing system with thermoelectric device and a cooling conduit.
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Evans, Robert E.; Hartman, Anthony L.; Suchter, Richard A.; Lefebvre, Gilles, Traceability for automated staining system.
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Evans, Robert E.; Hartman, Anthony L.; Suchter, Richard A.; Lefebvre, Gilles, Traceability for automated staining system.
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Polyakov, Evgeny; Garcia, Edward; Truebenbach, Eric L.; Merrow, Brian S.; Whitaker, Brian J., Transferring disk drives within disk drive testing systems.
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Polyakov, Evgeny; Garcia, Edward; Truebenbach, Eric L.; Merrow, Brian S.; Whitaker, Brian J., Transferring disk drives within disk drive testing systems.
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Polyakov, Evgeny; Garcia, Edward; Truebenbach, Eric L.; Merrow, Brian S.; Whitaker, Brian J., Transferring storage devices within storage device testing systems.
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Toscano, John; Polyakov, Evgeny; Garcia, Edward; Truebenbach, Eric L.; Merrow, Brian S.; Whitaker, Brian J., Transferring storage devices within storage device testing systems.
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Toscano, John; Polyakov, Evgeny; Garcia, Edward; Truebenbach, Eric L.; Merrow, Brian S.; Whitaker, Brian J., Transferring storage devices within storage device testing systems.
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Merrow, Brian S., Vibration isolation within disk drive testing systems.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표IPC 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 공고번호, 공고일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표출원인, 출원인국적, 출원인주소, 발명자, 발명자E, 발명자코드, 발명자주소, 발명자 우편번호, 발명자국적, 대표IPC, IPC코드, 요약, 미국특허분류, 대리인주소, 대리인코드, 대리인(한글), 대리인(영문), 국제공개일자, 국제공개번호, 국제출원일자, 국제출원번호, 우선권, 우선권주장일, 우선권국가, 우선권출원번호, 원출원일자, 원출원번호, 지정국, Citing Patents, Cited Patents
저장형식	Text(ASCII format) Excel format PIAS분석(.xls)
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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[미국특허] In situ heat induced antigen recovery and staining apparatus and method 원문보기

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[미국특허] In situ heat induced antigen recovery and staining apparatus and method 원문보기

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대표청구항 ▼

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