[특허]Nucleotide sequences for regulating gene expression in plant trichomes and constructs and methods utilizing same

[미국특허] Nucleotide sequences for regulating gene expression in plant trichomes and constructs and methods utilizing same 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C07H-021/04 C07H-021/00 C12N-015/82 A01H-005/00
출원번호	US-0561220 (2004-06-20)
등록번호	US-7498428 (2009-03-03)
국제출원번호	PCT/IL04/000549 (2004-06-20)
§371/§102 date	20060404 (20060404)
국제공개번호	WO04/111183 (2004-12-23)
발명자 / 주소	Ronen,Gil Rabinovich,Larisa Meissner,Rafael Karchi,Hagai
출원인 / 주소	Evogene Ltd.
인용정보	피인용 횟수 : 0 인용 특허 : 23

초록 ▼

A novel tomato plant derived regulatory sequence which comprises the nucleic acid set forth in SEQ ID NO:23 and which is active in trichomes is provided. Also provided are nucleic acid constructs comprising the tomato plant promoter and methods of using same for directing expression of the exogenous polynucleotide sequences in trichomes and transgenic plant cells and transgenic plants which comprise the nucleic acid constructs.

대표청구항 ▼

What is claimed is: 1. An isolated polynucleotide comprising the nucleic acid sequence as set forth in SEQ ID NO: 23. 2. A nucleic acid construct comprising the isolated polynucleotide of claim 1. 3. The nucleic acid construct of claim 2, wherein the nucleic acid construct further comprises at least one heterologous polynucleotide operably linked to the isolated polynucleotide. 4. The nucleic acid construct of claim 3, wherein the nucleic acid construct further comprises, a nucleic acid sequence encoding a peptide capable of directing transport of a polypeptide fused thereto into a subcellular compartment of a trichome. 5. The nucleic acid construct of claim 4, wherein said subcellular compartment of a trichome is a leucoplast. 6. A transgenic plant cell comprising the nucleic acid construct of claim 2. 7. A transgenic plant comprising the nucleic acid construct of claim 2. 8. A method of producing a polypeptide of interest in plant trichomes, the method comprising transforming a plant with the construct of claim 3; wherein the heterologous polynucleotide encodes said polypeptide of interest, and growing the plant so that the polypeptide is produced. 9. The method of claim 8, wherein the polypeptide encoded by said heterologous polynucleotide further comprises a peptide capable of directing transport of the polypeptide of interest into a subcellular compartment of the plant trichomes. 10. The method of claim 9, wherein said subcellular compartment of the plant trichomes is a leucoplast. 11. A method of producing a molecule of interest in plant trichomes, the method comprising transforming a plant with the construct of claim 3; wherein the heterologous polynucleotide encodes a polypeptide capable of directly or indirectly increasing the level of the molecule of interest, and growing the plant, thereby producing the molecule in the plant trichomes. 12. The method of claim 11, wherein said polypeptide is endogenously expressed in the plant trichomes. 13. The method of claim 11, wherein the polypeptide encoded by said heterologous polynucleotide further comprises a peptide capable of directing transport of the polypeptide into a subcellular compartment of the plant trichomes. 14. The method of claim 13, wherein said subcellular compartment of the plant trichomes is a leucoplast.

이 특허에 인용된 특허 (23) 인용/피인용 타임라인 분석

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Frossard Philippe M. (Palo Alto CA), Apo AI/CIII genomic polymorphisms predictive of atherosclerosis.
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Uzgiris Egidijus E. (Schenectady NY), Electrophoretic method of detecting antigen-antibody reaction.
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Joyce Gerald F. ; Breaker Ronald R., Enzymatic DNA molecules.
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Umbeck Paul F. (Madison WI), Genetic engineering of cotton plants and lines.
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Umbeck Paul F. (Madison WI), Genetic engineering of cotton plants and lines.
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Wands Jack R. (Waban MA) Zurawski ; Jr. Vincent R. (West Chester PA) Schoemaker ; Hubert J. P. (Newton MA), Immunoassay utilizing monoclonal high affinity IgM antibodies.
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Simons Malcolm J. (Fryerstown AUX), Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes.
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Hinchee Maud A. (Manchester MO) Connor-Ward Dannette (Olivette MO), Method for soybean transformation and regeneration.
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Hinchee Maud A. (Manchester MO) Connor-Ward Dannette (Olivette MO), Method for soybean transformation and regeneration.
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Corbin David R. (Chesterfield MO) Greenplate John T. (Manchester MO) Jennings Michael G. (Chesterfield MO) Purcell John P. (Ballwin MO) Sammons Robert D. (Defiance MO), Method of controlling insects.
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Smulson Mark E. (Washington DC) Bhatia Kishor (Arbutus MD) Huppi Konrad (Reston VA), Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for hu.
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Trojanowski John Q. (Philadelphia PA) Lee Virginia M-Y. (Philadelphia PA), Modified avidin-biotin technique.
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Bellet Dominique (Paris MA FRX) Wands Jack R. (Waban MA), Multiepitopic immunometric assay.
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Benfey Philip N. (New York NY) Chua Nam-Hai (Scarsdale NY), Plant promoters.
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Wagner, George J.; Gan, Susheng; Wang, Erming; Wang, Rui, Plant trichome gland specific promoter sequence.
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Mullis Kary B. (Kensington CA), Process for amplifying nucleic acid sequences.
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Piasio ; Roger N. ; Ryan ; James W. ; Woiszwillo ; James E., Reverse sandwich immunoassay.
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Benfey Philip N. (New York NY) Chua Nam-Hai (Scarsdale NY), Selective gene expression in plants.
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Gusella James F. (Framingham MA), Test for Huntington\s disease.
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Miles Laughton E. M. (Stanford CA), Universal reagent 2-site immunoradiometric assay using labelled anti (IgG).
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Nucleotide sequences for regulating gene expression in plant trichomes and constructs and methods utilizing same 원문보기