Methods of making and using composite arrays for the detection of a plurality of target analytes
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/53
G01N-033/566
G01N-033/00
출원번호
US-0767249
(2004-01-28)
등록번호
US-7510841
(2009-03-31)
발명자
/ 주소
Stuelpnagel,John
Chee,Mark
Auger,Steven
출원인 / 주소
Illumina, Inc.
대리인 / 주소
Knobbe Martens Olson & Bear LLP
인용정보
피인용 횟수 :
16인용 특허 :
149
초록▼
The invention relates to sensor compositions comprising a composite array of individual arrays, to allow for simultaneous processing of a number of samples. The invention further provides methods of making and using the composite arrays. The invention further provides a hybridization chamber for use
The invention relates to sensor compositions comprising a composite array of individual arrays, to allow for simultaneous processing of a number of samples. The invention further provides methods of making and using the composite arrays. The invention further provides a hybridization chamber for use with a composite array.
대표청구항▼
We claim: 1. A method of detecting the presence or absence of a plurality of different target analytes, comprising (a) providing a first substrate with a surface comprising a plurality of assay wells, wherein said assay wells contain sample solutions each having a plurality of different target anal
We claim: 1. A method of detecting the presence or absence of a plurality of different target analytes, comprising (a) providing a first substrate with a surface comprising a plurality of assay wells, wherein said assay wells contain sample solutions each having a plurality of different target analytes; (b) providing a second substrate comprising a plurality of array locations, each array location comprising a plurality of discrete sites on a projection, wherein said sites comprise different bioactive agents; (c) dipping the projections of said second substrate into said assay wells such that each array location of said second substrate contacts sample solution in a different well of said first substrate under conditions suitable for binding of said different target analytes to said different bioactive agents, thereby processing said sample solutions in parallel; and (d) detecting the presence or absence of said target analytes. 2. The method of claim 1, wherein said target analytes comprise nucleic acids or nucleic acid analogs. 3. The method of claim 2, wherein said nucleic acids comprise single nucleotide polymorphisms. 4. The method of claim 3, wherein said nucleic acids comprise single nucleotide polymorphisms obtained by multiplex PCR amplification. 5. The method of claim 2, wherein said nucleic acids are labeled with fluorochromes during PCR amplification. 6. The method of claim 1, wherein said bioactive agents are selected from the group consisting of peptides, peptide structural analogs, saccharides, fatty acids, steroids, purines, and pyrimidines. 7. The method of claim 1, wherein said array locations comprise from 10,000,000 to 2,000,000,000 bioactive agents per square centimeter. 8. The method of claim 1, wherein said array locations comprise from 100,000 to about 10,000,000 bioactive agents per square centimeter. 9. The method of claim 1, wherein said array locations comprise from 10,000 to about 100,000 bioactive agents per square centimeter. 10. The method of claim 1, wherein said bioactive agents are directly coupled to said array locations. 11. The method of claim 1, wherein said bioactive agents are attached to microspheres and wherein said microspheres. are associated with said array locations. 12. The method of claim 1, wherein said target analytes comprise decoder binding ligands. 13. The method of claim 1, wherein said target analyte is labeled. 14. The method of claim 13, wherein said label comprises an optical label. 15. The method of claim 14, wherein said optical label comprises. a fluorochrome. 16. The method of claim 1, wherein said detecting is done through the use of a change in optical signature. 17. The method of claim 1, further comprising quantitating differences in concentrations of said target analytes. 18. The method of claim 17, further comprising quantitating a specific mRNA. 19. The method of claim 18, comprising quantitating said specific mRNA in the presence of total cellular mRNA. 20. The method of claim 1, wherein said assay wells comprise wells of a microtiter plate. 21. The method of claim 1, wherein said plurality of assay wells comprises 96 wells. 22. The method of claim 1, wherein said plurality of assay wells comprises 384 wells. 23. The method of claim 1, wherein said plurality of assay wells comprises 1536 wells. 24. The method of claim 1, wherein optical signals generated at said discrete sites upon binding of said different target analytes to said different bioactive agents are detected. 25. The method of claim 24, wherein said different target analytes comprise labels and wherein said optical signal occurs as a result of said labels recruited to said sites by said target analytes binding said different bioactive agents. 26. The method of claim 24, wherein an enzyme generates species at said discrete sites that are optically detectable. 27. The method of claim 1, further comprising using said projections to stir said sample solutions in said assay wells.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (149)
Walt David R. (Lexington MA), Absorbance modulated fluorescence detection methods and sensors.
Hayes Donald J. (Plano TX) Wallace David B. (Dallas TX) Verlee Donald J. (Libertyville IL) Houseman Kenneth R. (Racine WI), Apparatus and process for reagent fluid dispensing and printing.
Honda Mitsuru (Tokyo JPX) Kawamura Takahisa (Yokohama JPX) Koike Atsushi (Chiba JPX) Murai Keiichi (Kashiwa JPX), Apparatus for producing a substrate having a surface with a plurality of spherical dimples for photoconductive members.
Palva Airi M. (Helsinki FIX) Ranki Tuula M. (Espoo FIX) Sderlund Hans E. (Espoo FIX), Arrays of alternating nucleic acid fragments for hybridization arrays.
Clark Frederic L. (Plano TX) Clift Gilbert (Mesquite TX) Hendrick Kendall B. (Southlake TX) Kanewske ; III William J. (Dallas TX) Lagocki Peter A. (Park Ridge IL) Martin Richard R. (Irving TX) Mitche, Automated continuous and random access analytical system.
Rutenberg Mark R. (Monsey NY) Hall Thomas L. (Pasadena CA) Domes Robert (Newbury Park CA) Chaban Richard (Ventura CA) Dulak Thomas (Newhall CA) Herriman James M. (New York NY) Porzio John (New York N, Automated cytological specimen classification system and method.
Andrus William A. (San Francisco CA) McCollum Christie D. (Foster City CA) Zon Gerald (San Carlos CA), Automated system for polynucleotide synthesis and purification.
Ekins Roger P. (Department of Molecular Endocrinology University College and Middlesex School of Medicine Mortimer Street London W1N 8AA GBX) Chu Frederick W. (Department of Molecular Endocrinology U, Binding assay employing labelled reagent.
Cunningham David D. (Lakemoor IL) Brontman Susan B. (Buffalo Grove IL) Geist Jill M. (Ingleside IL) Helgren R. Hayes (Mundelein IL) Henning Timothy P. (Vernon Hills IL) Johnson Kenneth S. (Buffalo Gr, Biosensor with a membrane containing biologically active material.
Hillman Robert S. (Cupertino CA) Cobb Michael E. (Sunnyvale CA) Allen Jimmy D. (Los Altos CA) Gibbons Ian (Menlo Park CA) Ostoich Vladimir E. (San Jose CA) Winfrey Laura J. (Belmont CA), Capillary flow device.
Hillman Robert S. (Cupertino CA) Cobb Michael E. (Sunnyvale CA) Allen Jimmy D. (Los Altos CA) Gibbons Ian (Menlo Park CA) Ostoich Vladimir E. (San Jose CA) Winfrey Laura J. (Belmont CA), Capillary flow device.
Still W. Clark (Clinton NY) Wigler Michael H. (Lloyd Harbor NY) Ohlmeyer Michael H. J. (Plainsboro NJ) Dillard Lawrence W. (Plainsboro NJ) Reader John C. (Princeton NJ), Complex combinatorial chemical libraries encoded with tags.
Goodman Barry D. (Baldwin NY) Wynne James J. (Mount Kisco NY) Kaufman Hershall W. (Setauket NY) Jacobs Jason M. (Baldwin NY), Dental procedures and apparatus using ultraviolet radiation.
Overbeck James W. ; Flowers Peter T. ; Montagu Jean I. ; Mace Myles L. ; Honkanen Peter, Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays.
Stapleton Marilyn J. (Durham NC) Thorpe Villette S. (Goldsboro NC) Jewett Warren R. (Cary NC), Devices for containing biological specimens for thermal processing.
Robinson David (Folkestone GB2) Bate Ernest (Great Chart GB2) Kellard Simon (Ashford GB2) Watson Mark (Ashford MA GB2) Mahan Donald E. (Grafton MA) Shimei Thomas M. (Franklin MA) Kearney Kevin R. (Wo, Diagnostics instrument.
Whitehead Thomas P. (70 Northumberland Road Leamington Spa ; Warwickshire GB2) Thorpe Gary H. G. H. (84 Newcombe Road Handsworth ; Birmingham GB2) Kricka Larry J. (16 Newent Road Northfield ; Birming, Dispensing device and recording apparatus.
Sutton Richard C. (Rochester NY) Ponticello Ignazio S. (Pittsford NY) Cummins Thomas J. (Rochester NY) Zander Dennis R. (Penfield NY) Donish William H. (Rochester NY), Element and method for nucleic acid amplification and detection using adhered probes.
Walt, David R.; Barnard, Steven M., Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample.
Gerdt David W. (P.O. Box 8175 Charlottesville VA 22906) Herr John C. (P.O. Box 8175 Charlottesville VA 22906), Fiber optic evanscent wave sensor for immunoassay.
Walt David R. (Lexington MA) Bernard Steven M. (Basel CHX), Fiber optic sensor, apparatus, and methods for detecting an organic analyte in a fluid or vapor sample.
Walt David R. (Lexington MA), Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held wi.
Walt David R. (Lexington MA), Fiber optic sensors, apparatus, and detection methods using fluid erodible controlled release polymers for delivery of r.
Pinkel Daniel ; Segraves Richard L. ; Zhai Ye Yz ; Albertson Donna G. ; Gray Joe, High density array fabrication and readout method for a fiber optic biosensor.
Kercso Joseph E. ; Sundberg Steven A. ; Wolk Jeffrey A. ; Toth Andrew W. ; Chow Calvin Y. H. ; Parce J. Wallace, High throughput microfluidic systems and methods.
Walt David R. (Lexington MA) Barnard Steven M. (Medford MA), Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a.
Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof.
Stabile Paul J. ; Ludington David Norman ; York Pamela Kay ; Rosen Arye ; Cherukuri Satyam Choudary ; Zanzucchi Peter John ; Heaney Paul, Massively parallel detection.
Sanford John C. (Geneva) DeVit Michael J. (Geneva NY) Bruner Ronald F. (Sewell NJ) Johnston Stephen A. (Durham NC), Method and apparatus for introducing biological substances into living cells.
Kolehmainen Seppo (P.O. Box 2805 Titusville FL 32780) Tarkkanen Veikko (Krijgersberglaan 25 6371 CA Schaesberg NLX 4), Method and apparatus for measurement of samples by luminescence.
John R. Stuelpnagel ; Mark S. Chee ; Richard J. Pytelewski ; Todd Alan Dickinson ; Gan G. Wang, Method and apparatus for retaining and presenting at least one microsphere array to solutions and/or to optical imaging systems.
Smyczek Peter J. (1837 N. Himount Blvd. Milwaukee WI 53208) Thompson John A. (2908 Ward Kline Rd. Myersville MD 21771), Method for conducting nucleic acid hybridization in chamber with precise fluid delivery.
Seifert Kevin R. (Forest Lake MN) Lynch Laurie (Eden Prairie MN) Johnson Debra K. (Andover MN) Kalla Jonathan (St. Louis Park MN) Fowler William (Minneapolis MN), Method for manufacturing fiber optic sensors.
Sutton Richard Calvin ; Ponticello Ignazio Salvatore ; Cummins Thomas Joseph ; Zander Dennis Roland ; Donish William Harold ; Chen Paul Hong-Dze ; Findlay John Bruce, Method for nucleic acid amplification and detection using adhered probes.
Walt David R. (Lexington MA) Barnard Steven M. (Medford MA), Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample.
Parce John W. (Palo Alto CA) McConnell Harden M. (Palo Alto CA) Humphries Gillian M. K. (Los Altos CA) Kercso Karen M. (Menlo Park CA) Owicki John C. (Palo Alto CA) Kercso Josef E. (Palo Alto CA), Methods for detecting the effect of cell affecting agents on living cells.
Rava Richard P. (San Jose CA) Fodor Stephen P. A. (Palo Alto CA) Trulson Mark (San Jose CA), Methods for making a device for concurrently processing multiple biological chip assays.
Lebl, Michal; Chee, Mark S.; Barnard, Steven M.; Heiner, David L.; Pokorny, Vit; Nibbe, Mark J.; Jones, Aaron Christopher, Oligonucleotide synthesizer.
Walt David R. ; Michael Karri L ; Chadha Suneet, Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations.
Walt David R. (Lexington MA) Kauer John S. (Weston MA), Optical sensor, optical sensing apparatus, and methods for detecting an analyte of interest using spectral recognition p.
Kirk Gregory L. (Skillman NJ) Brzezinski ; Jr. Joseph J. (Bangor PA) Chelsky Daniel (Moylan PA) Nichols Thirleen G. (Winterville NC) Ramaraj T. C. (Dayton NJ), Partitioning device.
Kearns Edward G. (North Haven CT) Roessler Norbert (Naperville IL) van Cauter Gustaaf (Middetown CT) Ukraincik Kreso (Cromwell CT), Radiometric analysis system for solid support samples.
Holen, James T.; Hicaro, Jr., Enrique; Putterman, Caryn G.; Huang, Tung-Ming; Merkh, Carl W., Reaction cartridge and carousel for biological sample analyzer.
Walt David R. (Lexington MA) Pantano Paul (Everett MA), Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy.
Dower William J. (Menlo Park CA) Barrett Ronald W. (Sunnyvale CA) Gallop Mark A. (Palo Alto CA) Needels Michael C. (Oakland CA), Synthesizing and screening molecular diversity.
Merkh Carl W. (Lindenhurst IL) Defreese James D. (Wildwood IL) Durley ; III ; deceased Benton A. (late of Antioch IL by Robert W. Durley ; executrix), Test array for performing assays.
Walt David R. (Lexington MA) Bronk Karen S. (Somerville MA), Thin film fiber optic sensor array and apparatus for concurrent viewing and chemical sensing of a sample.
Zarling David A. (Menlo Park CA) Rossi Michel J. (Lausanne CHX) Peppers Norman A. (Belmont CA) Kane James (Lawrenceville NJ) Faris Gregory W. (Menlo Park CA) Dyer Mark J. (San Jose CA) Ng Steve Y. (S, Up-converting reporters for biological and other assays using laser excitation techniques.
Winkler James L. (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Buchko Christopher J. (Palo Alto CA) Ross Debra A. (Fremont CA) Aldwin Lois (San Mateo CA), Very large scale immobilized polymer synthesis using mechanically directed flow paths.
Cozzette Stephen N. (Nepean CAX) Davis Graham (Plainsboro NJ) Itak Jeanne A. (North Brunswick NJ) Lauks Imants R. (Yardley PA) Mier Randall M. (Morrisville PA) Piznik Sylvia (Jackson NJ) Smit Nicolaa, Wholly microfabricated biosensors and process for the manufacture and use thereof.
Chou, Stephen Y.; Ding, Wei, Device and system for collecting and analyzing vapor condensate, particularly exhaled breath condensate, as well as method of using the same.
Rothberg, Jonathan M.; Hinz, Wolfgang; Johnson, Kim L.; Bustillo, James; Leamon, John; Schultz, Jonathan, Methods and apparatus for measuring analytes using large scale FET arrays.
Rajasekaran, John J.; Jayaraman, Vasanth; Wang, Tianhao; Bei, Kang; Krishnamurthy, Hari Krishnan, Methods, systems, and arrays for biomolecular analysis.
Rajasekaran, John J.; Jayaraman, Vasanth; Wang, Tianhao; Bei, Kang; Krishnamurthy, Hari Krishnan, Methods, systems, and arrays for biomolecular analysis.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.