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A flow cell for optical detector includes a flow cell body, a face-seal window, and a pressed illumination window. The flow cell body is formed of an inert material and includes large and small bores, and inlet and outlet passageways in fluid communication with the small bore. The face-seal window

A flow cell for optical detector includes a flow cell body, a face-seal window, and a pressed illumination window. The flow cell body is formed of an inert material and includes large and small bores, and inlet and outlet passageways in fluid communication with the small bore. The face-seal window is affixed within the large area aperture to form a liquid tight seal, while the pressed illumination window is press-set into the small bore. The pressed illumination window is spaced away from the face-seal window to form a sample chamber having a pathlength distance γ. The face-seal window and the pressed window provide an optical path through the sample chamber. Preferably, the flow cell body is formed of PEEK. Methods of forming and using the flow cell are also disclosed.

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What is claimed is: 1. A flow cell for an optical detector, said flow cell comprising: a flow cell body formed of an inert material, the flow cell body including a large area aperture and a small area aperture, the flow cell body also including inlet and outlet passageways in fluid communication wi

What is claimed is: 1. A flow cell for an optical detector, said flow cell comprising: a flow cell body formed of an inert material, the flow cell body including a large area aperture and a small area aperture, the flow cell body also including inlet and outlet passageways in fluid communication with the small area aperture; a face-seal window affixed within the large area aperture to form a first liquid tight seal; and a pressed window press-set into the small area aperture to form a second liquid tight seal within the small area aperture and spaced away from the face-seal window a pathlength distance γ thereby forming a sample chamber between the face-seal window and the pressed window; and wherein the face-seal window and the pressed window provide an optical path through the sample chamber. 2. A flow cell according to claim 1, wherein the inert material is plastic. 3. A flow cell according to claim 2, wherein the inert material is a polyetheretherketone. 4. A flow cell according to claim 1, wherein the liquid-tight seals withstand 1500 psi static pressure. 5. A flow cell according to claim 1, wherein at least one of the face-seal window and the pressed window is a lens to change the optical focus of light entering and/or exiting the cell. 6. A flow cell according to claim 1, wherein both of the face-seal window and the pressed window are lenses having inner surfaces that are flat and are oriented parallel to one another. 7. A flow cell according to claim 1, wherein the inlet and outlet passageways have a minimum inner dimension, and wherein a distance separating the pressed window from the face-seal window is less than the minimum inner dimension of said passageways. 8. A flow cell according to claim 7, wherein the distance separating the pressed window from the face-seal window defines a pathlength distance γ, and the pressed window has a diameter Φ; and wherein the flow cell has an illuminated volume V substantially equal to γ*π* (Φ/2)2. 9. A flow cell according to claim 8, wherein the illuminated volume V is less than approximately 0.7 μL. 10. A flow cell according to claim 1, wherein the large area aperture is a large diameter bore and the small area aperture is a small diameter bore. 11. A flow cell according to claim 10, wherein the sample chamber is formed by a portion of the small diameter bore. 12. A flow cell according to claim 11, further comprising inlet and outlet pockets extending along a side wall of the small diameter bore respectively interconnecting the inlet and outlet passageways with the sample chamber. 13. A flow cell according to claim 10, wherein the flow cell body includes an illumination aperture extending outwardly from the small diameter bore. 14. A flow cell according to claim 10, wherein the flow cell body includes a mounting bore extending outwardly from the large diameter bore. 15. A flow cell according to claim 14, further comprising a retainer received within the mounting bore for removably affixing the face-seal window within the large diameter bore. 16. A flow cell according to claim 15, wherein the retainer threadably engages the mounting bore. 17. A flow cell according to claim 15, wherein the retainer includes a viewing aperture extending therethrough, the viewing aperture being colinear with the pressed window and the face-seal window. 18. A flow cell according to claim 14, wherein the face seal window is removably affixed within the large diameter bore by an external plate. 19. In combination, a chromatography system including an absorbance detector for detecting absorbance of a sample, the absorbance detector comprising the flow cell of claim 1. 20. A method of optically analyzing a sample, the method comprising the steps: providing an optical analysis system having an absorbance detector that includes the flow cell of claim 1; filling the sample chamber with a sample; directing light of a known wavelength through the pressed window and the face-seal window; and detecting absorbance of light by the sample within the sample chamber. 21. The method according to claim 20, wherein the filling step includes flowing sample through the sample chamber at a rate of 100 ml/min per minute. 22. A flow cell according to claim 1, wherein the sample chamber comprises two side walls, a first wall defined by a surface of the face-seal window and a second wall defined by a surface of the pressed window. 23. A method of forming a flow cell, the method comprising the steps: providing a flow cell body formed of an inert material, the flow cell body including large and small area apertures, and inlet and outlet passageways in fluid communication with the small area aperture, wherein the large area aperture has a bottom; press-setting a pressed window into the small area aperture to form a liquid-tight seal therein, wherein the pressed window is press-set into the small area aperture a distance away from bottom of the large area aperture a distance D; and affixing a face-seal window into the large area aperture, wherein the pressed window and the face-seal window provide an optical path through the flow cell body of the flow cell and define a sample chamber therebetween having a pathlength distance γ substantially equal to distance D. 24. A method according to claim 23, wherein the providing step includes forming the flow cell body of polyetheretherketone. 25. A method according to claim 23, the method further comprising the step: utilizing a stepped press setting fixture to determine the distance D. 26. A method according to claim 23, wherein the inserting step includes press-setting the pressed window into the small area aperture through the large area aperture. 27. A method according to claim 26, wherein the inlet and outlet passageways have a minimum inner dimension, and wherein the distance γ is less than the minimum inner dimension of said passageways. 28. A method according to claim 23, wherein the providing step includes providing the flow cell body with an illumination aperture extending outwardly from the small area aperture. 29. A method according to claim 23, wherein the providing step includes providing the flow cell body with a mounting bore extending outwardly from the large area aperture. 30. A method according to claim 29, wherein the providing step includes providing a retainer received within the large diameter portion for removably affixing the face-seal within the large area aperture. 31. A method according to claim 30, wherein the affixing step includes threadably engaging the retainer within the large area aperture. 32. A method according to claim 23, wherein the sample chamber comprises two side walls, a first wall defined by a surface of the face-seal window and a second wall defined by a surface of the pressed window.