The invention relates to a method of producing hyaluronic acid including (1) a step of transforming a plant cell using an expression recombinant vector including (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence having one or more amino ac
The invention relates to a method of producing hyaluronic acid including (1) a step of transforming a plant cell using an expression recombinant vector including (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence having one or more amino acid deletions, substitutions, additions or insertions in an amino acid sequence of the hyaluronic acid synthase and having an activity of synthesizing the hyaluronic acid, (2) a step of growing a transformant obtained by transformation, and (3) a step of separating the hyaluronic acid produced by the transformant.
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The invention claimed is: 1. A method of producing hyaluronic acid comprising (1) a step of transforming a plant cell using an expression recombinant vector comprising (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic ac
The invention claimed is: 1. A method of producing hyaluronic acid comprising (1) a step of transforming a plant cell using an expression recombinant vector comprising (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase, (2) a step of growing a transformant obtained by transformation of step (1), and (3) a step of separating the hyaluronic acid produced by the transformant, wherein the hyaluronic acid synthase synthase is derived from a chlorella virus. 2. A method of producing hyaluronic acid comprising (1) a step of transforming a plant using an expression recombinant vector comprising (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase, (2) a step of growing a transformant obtained by transformation of step (1), and (3) a step of separating the hyaluronic acid produced by the transformant, wherein the hyaluronic acid is derived from a chlorella virus. 3. A method of making a transformed plant cell having an ability of producing hyaluronic acid comprising a step of transforming a plant cell using an expression recombinant vector comprising (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase, wherein the hyaluronic acid synthase is derived from a chlorella virus. 4. A method of making a transformed plant having an ability of producing hyaluronic acid comprising a step of transforming a plant cell using an expression recombinant vector comprising (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase, wherein the hyaluronic acid synthase is derived from a chlorella virus. 5. The method of claim 4 wherein the expression recombinant vector further comprises an organ-specific or tissue-specific promoter, wherein the resulting transformed plant has the ability of producing the organ-specific or tissue-specific hyaluronic acid. 6. A transformed plant cell having an ability of producing hyaluronic acid, obtained by transforming a plant cell using an expression recombinant vector comprising (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase, wherein the hyaluronic acid synthase is derived from a chlorella virus. 7. A transformed plant, or a progeny thereof or an organ thereof or a tissue thereof, having the ability of producing hyaluronic acid, wherein said plant is obtained by transforming a plant using an expression recombinant vector comprising (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase, wherein the hyaluronic acid synthase is derived from a chlorella virus. 8. The transformed plant or the progeny thereof or the organ thereof or the tissue thereof of claim 7, wherein the plant is selected from the group consisting of angiosperm, gymnosperm, pteridophyte and bryophyte. 9. The transformed plant or the progeny thereof or the organ thereof or the tissue thereof of claim 7, wherein the organ is one or more organs selected from the group consisting of a root, a stem, a rootstock, a leaf, a flower, a root truncation, a seed, and a shoot apex. 10. The transformed plant or the progeny thereof or the organ thereof or the tissue thereof of claim 7, wherein the tissue is one or more tissues selected from the group consisting of an epidermis, a phloem, a parenchyma, a xylem, and a vascular bundle. 11. The transformed plant or the progeny thereof or organ thereof or the tissue thereof of claim 7, wherein the expression recombinant vector further comprises an organ-specific or tissue-specific promoter, wherein the resulting transformed plant has the ability of producing the organ-specific or tissue-specific hyaluronic acid.
Smeekens Josephus Christianus Maria,NLX ; Ebskamp Michael Johannes Marcus,NLX ; Geerts Hendrikus Andrianus Maria,NLX ; Weisbeek Petrus Jacobus,NLX, Production of oligosaccharides in transgenic plants.
Delgado González, Raquel; Soley Astals, Albert; Courtois, Anthony; Thollas, Bertrand, Exopolysaccharide for the treatment and/or care of the skin, mucous membranes and/or nails.
Wetcholowsky, Ingo; Rieck, Heiko; Labourdette, Gilbert; Geraldes, Jose Augusto, Use of succinate dehydrogenase inhibitors for controlling Sclerotinia ssp.
Wetcholowsky, Ingo; Rieck, Heiko; Labourdette, Gilbert; Geraldes, Jose Augusto, Use of succinate dehydrogenase inhibitors for controlling Sclerotinia ssp.
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