[미국특허]
Method of preparing a conditioned medium from a confluent stromal cell culture
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-005/00
C12N-005/06
C12N-005/08
C12N-011/08
C12M-003/00
A61F-002/00
출원번호
UP-0102623
(2005-04-11)
등록번호
US-7678573
(2010-04-21)
발명자
/ 주소
Merchav, Shoshana
Meretski, Shai
Zipori, Dov
Kadouri, Avinoam
출원인 / 주소
Pluristem Ltd.
인용정보
피인용 횟수 :
9인용 특허 :
173
초록▼
A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under contin
A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding undifferentiated hemopoietic stem cells; and (b) when a desired stromal cell density has been achieved, collecting medium from the stationary phase plug-flow bioreactor, thereby obtaining the stromal cell conditioned medium useful in expanding the undifferentiated hemopoietic stem cells.
대표청구항▼
What is claimed is: 1. A method of preparing a conditioned medium from a confluent stromal cell culture, the method comprising: (a) culturing stromal cells to confluence in a stationary phase plug-flow bioreactor under continuous flow of medium on a substrate in the form of a sheet, said substrate
What is claimed is: 1. A method of preparing a conditioned medium from a confluent stromal cell culture, the method comprising: (a) culturing stromal cells to confluence in a stationary phase plug-flow bioreactor under continuous flow of medium on a substrate in the form of a sheet, said substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network to obtain a stationary phase three-dimensional confluent stromal cell culture, wherein said stromal cell culture is grown to a density of at least 5×106 cells per a cubic centimeter of said substrate; and (b) separating and collecting a stromal cell conditioned medium from said stationary phase confluent stromal cell culture; thereby obtaining the conditioned medium, said medium useful in expanding an undifferentiated hemopoietic stem cell culture to increase the number of the undifferentiated hemopoietic stem cells. 2. The method of claim 1, wherein said stromal cell culture is grown to a density of at least 107 cells per a cubic centimeter of said substrate. 3. The method of claim 1, wherein said fibrous matrix forms a pore volume as a percentage of total volume of from 40 to 95% and a pore size of from 10 microns to 100 microns. 4. The method of claim 1, wherein said matrix is made of fiber selected from the group consisting of flat, non-round, and hollow fibers and mixtures thereof, said fibers being of from 0.5 microns to 50 microns in diameter or width. 5. The method of claim 1, wherein said matrix is composed of ribbon formed fibers having a width of from 2 microns to 20 microns, and wherein the ratio of width to thickness of the fibers is at least 2:1. 6. The method of claim 1, wherein said matrix has a pore volume as a percentage of total volume of from 60 to 95%. 7. The method of claim 1, wherein the matrix has a height of 50-1000 μm. 8. The method of claim 1, wherein the matrix is formed from a material selected from the group consisting of polyesters, polyalkylenes, polyfluorochloroethylenes, polyvinyl chloride, polystyrene, polysulfones, cellulose acetate, glass fibers, and inert metal fibers. 9. The method of claim 1, wherein the matrix is in a shape selected from the group consisting of squares, rings, discs, and cruciforms. 10. The method of claim 1, wherein the matrix is in the form of a disc. 11. The method of claim 1, wherein the matrix is coated with poly-D-lysine. 12. The method of claim 1, wherein said stromal cell culture comprises a primary culture. 13. The method of claim 1, wherein said stromal cell culture comprises a culture of a stromal cell line. 14. The method of claim 1, wherein said stromal cell conditioned medium is devoid of added cytokines. 15. The method of claim 1, wherein a rate of said continuous flow is in a range of 0.1 to 25 ml/minute. 16. The method of claim 1, wherein a rate of said continuous flow is in a range of 1 to 10 ml/minute.
Koning Gerrit (Vries FL NLX) Schroeppel Edward A. (Miramar FL), Apparatus and method for adjusting heart/pacer rate relative to right ventricular systolic pressure to obtain a required.
Nappholz Tibor A. (Englewood CO) Koestner Ken (Englewood CO), Apparatus and method for detecting abnormal cardiac rhythms in dual chamber arrhythmia control system.
Carney James K. (Eden Prairie MN), Apparatus and method for determining a plurality of hemodynamic variables from a single, chroniclaly implanted absolute.
Roline, Glenn M.; Nichols, Lucy M.; Bennett, Tommy D.; Thompson, David L., Automatic cardiac capture restoration and threshold-seeking method and apparatus.
Lu Richard M. T. (Highlands Ranch CO) Steinhaus Bruce M. (Parker CO) Crosby Peter A. (Greenwood Village CO) Nolan Janice (Conifer CO), Automatic ventricular pacing pulse threshold determination utilizing an external programmer and a surface electrocardiog.
Alt Eckhard (Eichendorffstrasse 52 8012 Ottobrunn DEX), Cardiac and pulmonary physiological analysis via intracardiac measurements with a single sensor.
Salo Rodney W. (Fridley MN) Spinelli Julio C. (Shoreview MN) Tockman Bruce A. (Minneapolis MN), Cardiac stimulating apparatus and method for heart failure therapy.
Mirowski Mieczyslaw (Owings Mills MD) Mower Morton M. (Baltimore MD) Denniston ; III Rollin H. (Anoka MN), Cardioverting device having single intravascular catheter electrode system and method for its use.
Mirowski Mieczyslaw (Rte. 3 ; Velvet Valley Way Owings Mills MD 21117) Mower Morton M. (Baltimore MD) Langer Alois A. (Pittsburgh PA), Cardioverting device with stored energy selecting means and discharge initiating means, and related method.
Stephanopoulos Gregory (Winchester MA) Singhvi Rahul (Cambridge MA) Park Seujeung (Somerville MA) Flytzani-Stephanopoulos Maria (Winchester MA) Applegate Mark A. (San Diego CA), Cell-culturing apparatus and method employing a macroporous support.
Langer Alois A. (2405 Velvet Valley Way Pittsburgh PA) Heilman Marlin S. (2405 Velvet Valley Way Gibsonia PA) Mower Morton M. (2405 Velvet Valley Way Baltimore MD) Mirowski Mieczyslaw (2405 Velvet Va, Circuit for monitoring a heart and for effecting cardioversion of a needy heart.
Edwards Stuart D. (San Ramon CA) Franz Michael R. (Washington DC) Thompson Russel B. (San Leandro CA) Stern Roger A. (Cupertino CA), Combination monophasic action potential/ablation catheter and high-performance filter system.
Zacouto Fred (16 rue de la Convention 75 015 Paris FRX), Device for protection against blood-related disorders, notably thromboses, embolisms, vascular spasms, hemorrhages, hemo.
Shapland James E. (Shoreview MN) Knudson Mark B. (Shoreview MN) Shimada Jin (Falcon Heights MN) Hildebrand Keith R. (Houlton WI), Drug delivery apparatus and method.
Dempsey Michael K. (Acton MA) Kotfila Mark S. (Chelmsford MA) Snyder Robert J. (Westford MA), Flexible patient monitoring system featuring a multiport transmitter.
Wands Jack R. (Waban MA) Zurawski ; Jr. Vincent R. (West Chester PA) Schoemaker ; Hubert J. P. (Newton MA), Immunoassay utilizing monoclonal high affinity IgM antibodies.
Bocchi David E. (Vadnais Heights MN) Laackman Jeffrey T. (Isanti MN) Bach ; Jr. Stanley M. (Shoreview MN), Implantable N-phasic defibrillator output bridge circuit.
Salo Rodney W. (Fridley MN) Tockman Bruce A. (Minneapolis MN) Mower Morton M. (Edina MN), Implantable cardiac function monitor and stimulator for diagnosis and therapy delivery.
Duncan James L. (Alpharetta GA), Implantable cardiac stimulating device and method for administering synchronized cardioversion shock therapy to provide.
Haluska Edward A. (Angleton TX) Whistler Stephen J. (Lake Jackson TX) Baker ; Jr. Ross G. (Houston TX) Calfee Richard V. (Houston TX), Implantable cardiac stimulator for detection and treatment of ventricular arrhythmias.
Engle William R. (Blaine MN) Moore ; Jr. E. Neil (Wallingford PA) Spear ; Jr. Joseph F. (Philadelphia PA) Rockland Ronald H. (Parsippany NJ), Implantable cardioverter.
Adams Theodore P. (Edina MN) Kroll Mark W. (Minnetonka MN), Implantable defibrillator system and method having successive changeable defibrillation waveforms.
Obel Israel W. P. (Johannesburg ZAX) Bourgeois Ivan (Verviers BEX), Implantable electrical nerve stimulator/pacemaker with ischemia for decreasing cardiac workload.
Heilman Marlin S. (Gibsonia PA) Langer Alois A. (Pittsburgh PA) Mirowski Mieczyslaw (Owings Mills MD) Mower Morton M. (Baltimore MD) Reilly David M. (Pittsburgh PA), Implantable electrodes for accomplishing ventricular defibrillation and pacing and method of electrode implantation and.
Langer Alois A. (2405 Velvet Valley Way Pittsburgh PA) Kolenik Steve A. (2405 Velvet Valley Way Leechburg PA) Heilman Marlin S. (2405 Velvet Valley Way Gibsonia PA) Mirowski Mieczyslaw (2405 Velvet V, Implantable heart stimulator and stimulation method.
Prutchi David (Lake Jackson TX) Paul Patrick J. (Lake Jackson TX), Implantable medical device with enclosed physiological parameter sensors or telemetry link.
Dahl Roger W. (Andover MN) Heil ; Jr. Ronald W. (Roseville MN) Beatty Graydon E. (New Brighton MN) Mower Morton M. (Lutherville MD), Low energy defibrillation electrode.
Stroetmann Brigitte (Uttenreuth DEX) Holmstrom Nils (Jarfalla SEX) Kallert Siegfried (Erlangen DEX) Bowald Staffan (Almunge SEX), Method and apparatus for cardiac therapy by stimulation of a physiological representative of the parasympathetic nervous.
Hahn Stephen J. (Roseville MN) Swanson David K. (Mountain View CA), Method and apparatus for defibrillation using a multiphasic truncated exponential waveform.
Langer Alois A. (2405 Velvet Valley Way Pittsburgh PA) Heilman Marlin S. (2405 Velvet Valley Way Gibsonia PA) Mower Morton M. (2405 Velvet Valley Way Baltimore MD) Mirowski Mieczyslaw (2405 Velvet Va, Method and apparatus for monitoring heart activity, detecting abnormalities, and cardioverting a malfunctioning heart.
Borst Cornelius,NLX ; Mansvelt Beck Hendricus J.,NLX ; Grundeman Paul F.,NLX ; Jansen Erik W. L.,NLX, Method and apparatus for temporarily immobilizing a local area of tissue.
Combs William J. (Eden Prairie MN) Duffin Edwin G. (New Brighton MN) Rao T. V. (Coon Rapids MN), Method and apparatus for treatment of atrial fibrillation.
Mower Morton M., Method and apparatus to allow cyclic pacing at an average rate just above the intrinsic heart rate so as to maximize inotropic pacing effects at minimal heart rates.
Hill Michael R. S. ; Jonkman Kenneth R., Method and device for electronically controlling the beating of a heart using venous electrical stimulation of nerve fibers.
Forester George V. (Ottawa CAX) Mortimer Alan J. (Gloucester CAX) Roy Orest Z. (Ottawa CAX), Method and device for enhancement of cardiac contractility.
Pohndorf Peter J. (Miami Shores FL) Schroeppel Edward A. (Miramar FL), Method and means of electrode selection for pacemaker with multielectrode leads.
Mower Morton M. (5501 Village Dr. ; #302 Edina MN 55435) Chen Peng S. (474 Paulette Pl. La Canada CA 91011), Method for establishing defibrillation threshold for a cardiac defibrillator.
Shapland James E. (Shoreview MN) Hildebrandt Keith R. (Houlton WI) Knudson Mark B. (Shoreview MN), Method for iontophoretically delivering drug adjacent to a heart.
Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI), Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultur.
Smulson Mark E. (Washington DC) Bhatia Kishor (Arbutus MD) Huppi Konrad (Reston VA), Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for hu.
Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI), Methods for the ex vivo replication of human stem cells and/or expansion of human progenitor cells.
Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI), Methods, compositions and devices for growing human hematopoietic cells.
Addiego William P. (Corning NY) Swaroop Srinivas H. (Painted Post NY) Williams Jimmie L. (Painted Post NY) Wusirika Raja R. (Painted Post NY), Multi-stage TWC system.
Fayram Timothy A. (Gilroy CA) Benedict George J. (Santa Cruz CA), Optical blood flow measurement apparatus and method and implantable defibrillator incorporating same.
Walters Robert A. (Murrysville PA), Pacemaker with circuit for pulse width modulating stimulus pulses in accordance with programmed parameter control states.
Brownlee Robert R. (State College PA) Hughes ; Jr. Howard C. (Cornwall PA) Neff Paul H. (Bellefonte PA) Tyers G. Frank O. (Vancouver CAX), Pacer internal cardiac electrogram sensing system.
Racchini Joel R. (Edina MN) Shapland James E. (Shoreview MN) Knudson Mark B. (Shoreview MN) Shimada Jin (Falcon Heights MN) Hildebrand Keith R. (Houlton WI), Porous balloon for selective dilatation and drug delivery.
Bennett Tom D. (Shoreview MN) Lundstrom ; Jr. Russell L. (Minneapolis MN) Stein Paul M. (Maple Grove MN), Post-extrasystolic potentiation stimulation with physiologic sensor feedback.
Spano Mark L. (Laurel MD) Ditto William L. (Wooster OH) Garfinkel Alan (Venice CA) Weiss James N. (Encino CA), Real time stabilizing system for pulsating activity.
Taylor Charles S. ; Benetti Federico J.,ARX ; Matheny Robert G., Surgical devices for imposing a negative pressure to stabilize cardiac tissue during surgery.
Clare Christopher R. (Los Altos CA) Imran Mir A. (Palo Alto CA), Switch apparatus and method for switching between multiple electrodes for diagnostic and therapeutic procedures.
Bornzin Gene A. (Camarillo CA) Arambula Elia R. (Artesia CA) Florio Joseph J. (Sunland CA), System and method for automatically determining the slope of a transfer function for a rate-responsive cardiac pacemaker.
Bigagli Roberto (Milan ITX) Grillenzoni Gianni (Milan ITX) Landi Mauro (Milan ITX) Arcari Augusto (Milan ITX), Two-component device for the administration of drugs.
Bach ; Jr. Stanley M. (Shoreview MN) Shapland J. Edward (Shoreview MN) Lang Douglas J. (Arden Hills MN) Dahl Roger W. (Andover MN), Unitary intravascular defibrillating catheter with separate bipolar sensing.
Warren Jay A. (Camino CA) Millerhagen Jay O. (Shoreview MN) Spinelli Julio C. (Shoreview MN), Variable rate pacemaker having upper rate limit governor based on hemodynamic performance.
Kepler Charles E. (Manchester CT) Deblois Raymond L. (Tolland CT) Spadaccini Louis J. (Manchester CT), Wake stabilized supersonic combustion ram cannon.
Meiron, Moran; Toren, Amir; Ofir, Rachel; Aberman, Zami; Drori-Carmi, Nirit, Adherent cells from adipose or placenta tissues and use thereof in therapy.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.