초록 ▼

The invention relates to analytical methods in which the partition of a labeled substance between a liquid and a solid phase is determined. The assays include solid-phase reagents which can be particulate or monolithic such as, for example, a coated tube. Assays of this type are known per se to the

The invention relates to analytical methods in which the partition of a labeled substance between a liquid and a solid phase is determined. The assays include solid-phase reagents which can be particulate or monolithic such as, for example, a coated tube. Assays of this type are known per se to the person skilled in the art and include immunoassays and immunometric assays.

대표청구항 ▼

The invention claimed is: 1. A heterogeneous immunochemical method for the determination of an analyte in a sample in a liquid medium, comprising: a) incubating the sample in a reaction vessel with a particulate solid-phase on which a first binding partner which specifically binds to the analyte is

The invention claimed is: 1. A heterogeneous immunochemical method for the determination of an analyte in a sample in a liquid medium, comprising: a) incubating the sample in a reaction vessel with a particulate solid-phase on which a first binding partner which specifically binds to the analyte is immobilized; wherein the particles of the particulate solid phase have a size of 0.1-5.0 μm; and with a second binding partner which specifically binds to the analyte, wherein a fluorescent or chemiluminescent label is conjugated to the second binding partner, and wherein after addition of the solid-phase and the second binding partner the solid-phase is suspended in the liquid medium, whereby the second binding partner is partitioned between the liquid medium and the particulate solid-phase and the solid-phase is subjected to magnetic or gravitational attraction to attract the solid-phase in the liquid medium to an interior surface of the reaction vessel; b) removing the first liquid medium from the solid-phase prior to adding a second liquid medium to the solid-phase; c) resuspending the solid-phase in the second liquid medium; d) adding a reagent A, which causes a separation of the solid-phase and the label by elimination of at least one intermolecular linkage which is essentially composed of hydrogen bonds and ionic, hydrophobic and van der Waals interactions; e) removing the solid-phase by magnetic or gravitational attraction; and f) determining the amount of analyte in the sample, wherein the reagent A contains in aqueous solution one substance selected from the group of reagents consisting of: a chaotropic agent, and a buffer for adjusting the pH to a range of 1.5 to 3; wherein a) through f) are performed in sequential order. 2. The method of claim 1, wherein the particulate solid-phase is magnetically attractable. 3. The method of claim 1, wherein the label is chemiluminescent. 4. The method of claim 1, wherein the reagent A contains said buffer for adjusting the pH to a range of pH 1.5 to 3 in a concentration of 0.1 to 1 mol/l; and an ionic detergent. 5. The method of claim 4, wherein the ionic detergent has a concentration of 0.01 to 10% (w/v). 6. The method of claim 4, wherein the ionic detergent is sodium dodecylsulfate. 7. The method of claim 4, wherein the buffer is glycine. 8. The method of claim 5, wherein said detergent concentration is 1% (w/v). 9. The method of claim 1, wherein the particles of the particulate solid phase have a size of 0.2-2.5 μm. 10. The method of claim 1, wherein the particles of the particulate solid phase have a size of 0.2-1.0 μm. 11. A heterogeneous immunochemical method for the determination of an analyte in a sample in a liquid medium, comprising: a) incubating the sample in a reaction vessel with a particulate solid-phase on which a first binding partner which specifically binds to the analyte is immobilized; wherein the particles of the particulate solid phase have a size of 0.1-5.0 μm; and with a second binding partner which specifically binds to the analyte, wherein a fluorescent or chemiluminescent label is conjugated to the second binding partner, and wherein after addition of the solid-phase and the second binding partner the solid-phase is suspended in the liquid medium, whereby the second binding partner is partitioned between the liquid medium and the particulate solid-phase and the solid-phase is subjected to magnetic or gravitational attraction to attract the solid-phase in the liquid medium to an interior surface of the reaction sample; b) removing the first liquid medium from the solid-phase prior to adding a second liquid medium to the solid-phase; c) resuspending the solid-phase in the second liquid medium; d) adding a reagent A, which causes a separation of the solid-phase and the label by elimination of at least one intermolecular linkage which is essentially composed of hydrogen bonds and ionic, hydrophobic and van der Waals interactions; e) removing the solid-phase by magnetic or gravitational attraction; and f) determining the amount of analyte in the sample, wherein the reagent A contains in an aqueous solution one substance selected from the group of reagents consisting of: KSCN, guanidinium hydrochloride, NaClO4, urea, propionic acid, dimethylformamide, and glycine for adjusting the pH to a range of 1.5 to 3; wherein a) through f) are performed in sequential order.