초록 ▼

There is disclosed novel mutant IGFBP-3 polypeptides and fragments thereof that have either no binding, or diminished binding to IGFs, yet retain their ability to bind to the human IGFBP-3 receptor (“P4.33”). The present invention provides novel mutant IGFBP-3 nucleic acid sequences, a

There is disclosed novel mutant IGFBP-3 polypeptides and fragments thereof that have either no binding, or diminished binding to IGFs, yet retain their ability to bind to the human IGFBP-3 receptor (“P4.33”). The present invention provides novel mutant IGFBP-3 nucleic acid sequences, and expression systems. Additional exemplary embodiments provide for screening assays for identifying IGFBP-3 receptor antagonists or agonists, methods for modulating IGF-independent IGFBP-3 responses of cells expressing IGFBP-3 receptors, methods for inducing or potentiating apoptosis of cells expressing IGFBP-3 receptors, methods for treating solid tumors having cells expressing IGFBP-3 receptors, and compositions comprising polypeptides having either no binding, or diminished binding to IGFs, yet retain their ability to bind to the IGFBP-3 receptor.

대표청구항 ▼

We claim: 1. A purified polypeptide, the amino acid sequence of which comprises SEQ ID NO: 7, wherein the polypeptide binds to the extracellular domain of human Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) receptor. 2. The purified polypeptide of claim 1, wherein the polypeptide is from

We claim: 1. A purified polypeptide, the amino acid sequence of which comprises SEQ ID NO: 7, wherein the polypeptide binds to the extracellular domain of human Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) receptor. 2. The purified polypeptide of claim 1, wherein the polypeptide is from 87 to about 264 amino acids in length. 3. The purified polypeptide of claim 1, wherein the polypeptide is, or comprises a recombinant variant of human IGFBP-3, wherein the variant consists of the double glycine substitutions at amino acid positions 56 and 80 of SEQ ID NO:7. 4. A composition comprising a polypeptide, the amino acid sequence of which comprises SE ID NO:7, wherein the polypeptide binds to the extracellular domain of the human IGFBP-3 receptor. 5. The composition of claim 4, wherein the polypeptide is from 87 to about 264 amino acids in length. 6. The composition of claim 4, wherein the polypeptide is, or comprises a recombinant variant of human IGFBP-3, wherein the variant consists of the double glycine substitutions at amino acid positions 56 and 80 of SEQ ID NO:7.