[특허]Process for making transplantable cardiomyocytes from human embryonic stem cells

[미국특허] Process for making transplantable cardiomyocytes from human embryonic stem cells 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12N-005/08
출원번호	UP-0805099 (2004-03-19)
등록번호	US-7732199 (2010-06-29)
발명자 / 주소	Xu, Chunhui Gold, Joseph D.
출원인 / 주소	Geron Corporation
대리인 / 주소	Mittler, E. Stewart
인용정보	피인용 횟수 : 9 인용 특허 : 15

초록 ▼

This invention provides populations human cells of the cardiomyocyte lineage. The cells are obtained by causing cultures of pluripotent stem cells to differentiate in vitro, and then harvesting cells with certain phenotypic features. Differentiated cells bear cell surface and morphologic markers characteristic of cardiomyocytes, and a proportion of them undergo spontaneous periodic contraction. Highly enriched populations of cardiomyocytes and their replicating precursors can be obtained, suitable for use in a variety of applications, such as drug screening and therapy for cardiac disease.

대표청구항 ▼

The invention claimed is: 1. A method of generating a cell composition containing cells of cardiomyocyte lineage from human embryonic stem (hES) cells, comprising: a) initiating differentiation of the hES cells in suspension culture by forming embryoid bodies in a media comprising serum; b) culturing the initiated cells so that they differentiate into cells that undergo spontaneous contraction; c) harvesting differentiated cells that demonstrate spontaneous contraction; d) separating the harvested cells into fractions based on density wherein the cells are present in a fraction having a density of about 1.05 g/ml, a fraction having a density of about 1.075 g/ml, a fraction including an upper interface of a gradient comprising a fraction having a density of about 1.05 g/ml, and a fraction having a density of about 1.075 g/ml, and a fraction including a lower interface of a gradient comprising a fraction having a density of about 1.05 g/ml, and a fraction having a density of about 1.075 g/ml; and e) isolating the cell fractions containing cells that express cardiac troponin I (cTnI), cardiac troponin T (cTnT), or atrial natriuretic factor (ANF) from an endogenous gene; thereby generating a cell composition comprising cardiomyocyte lineage cells. 2. The method of claim 1, wherein the embryoid bodies are plated onto a surface coated with gelatin or extracellular matrix. 3. The method of claim 1, wherein the cells are differentiated in the presence of a nucleotide analog that affects DNA methylation. 4. The method of claim 1, wherein the cells are differentiated in a growth environment comprising activin, an insulin-like growth factor and a member of the TGFIβ family. 5. The method of claim 1, wherein the cells are differentiated in a growth environment containing 20% serum or serum substitute. 6. The method of claim 1, wherein the harvested cells are separated by density centrifugation. 7. The method of claim 1, further comprising culturing the collected cells for at least 1 week in a medium containing a compound capable of forming a high energy phosphate bond, an acyl group carrier molecule, and a cardiomyocyte calcium channel modulator. 8. The method of claim 7, further comprising culturing the collected cells for at least 1 week in a medium containing creatine, carnitine, or taurine.

이 특허에 인용된 특허 (15) 인용/피인용 타임라인 분석

Walsh,Kenneth, Akt compositions for enhancing survival of cells.
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Xiao, Yong-Fu; Morgan, James P., Cardiac function comprising implantation of embryonic stem cell in which differentiation has been initiated.
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Mark F. Pittenger ; Stephen L. Gordon ; Alastair Morgan Mackay, Cardiac muscle regeneration using mesenchymal stem cells.
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Xu,Chunhui, Cardiomyocyte precursors from human embryonic stem cells.
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Gold,Joseph D.; Hassanipour,Mohammad; Collins,Lila R.; Xu,Chunhui, Direct differentiation method for making cardiomyocytes from human embryonic stem cells.
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Franz Wolfgang-Michael,DEX ; Wobus Anna M.,DEX, Embryonal cardiac muscle cells, their preparation and their use.
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Gearhart John D. ; Shamblott Michael Joseph, Human embryonic germ cell line and methods of use.
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Smith David A. (3244 Belle Ct. Royal Oak MI 48073) Townsend Laurace E. (868 Whittier Grosse Pointe Park MI 48230), Method of isolation, culture and proliferation of human atrial myocytes.
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Field Loren J., Myocardial grafts and cellular compositions.
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Field Loren J., Myocardial grafts and cellular compositions.
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Loren J. Field, Myocardial grafts and cellular compositions useful for same.
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Thomson James A., Primate embryonic stem cells.
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Cech Thomas R. ; Lingner Joachim,CHX ; Nakamura Toru ; Chapman Karen B. ; Morin Gregg B. ; Harley Calvin B. ; Andrews William H., Telomerase.
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Mickle Donald A. G.,CAX ; Li Ren-Ke,CAX ; Weisel Richard D.,CAX, Transplants for myocardial scars.
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Mickle Donald A. G.,CAXITX M5M 2X9 ; Li Ren-Ke,CAXITX M1V 1B4 ; Weisel Richard D.,CAXITX M4X 1S8, Transplants for myocardial scars and methods and cellular preparations.
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이 특허를 인용한 특허 (9) 인용/피인용 타임라인 분석

Colton, Clark K.; Gleason, Karen K.; Coclite, Anna M.; Dilenno, Amanda R., Articles and methods for stem cell differentiation.
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Terzic, Andre; Behfar, Atta, Compositions and methods for obtaining cells to treat heart tissue.
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Terzic, Andre; Behfar, Atta, Compositions and methods for obtaining cells to treat heart tissue.
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Colton, Clark K.; Dilenno, Amanda; Millman, Jeffrey R., Method for differentiating human embryonic stem cells into β-cells for the treatment of type I diabetes.
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Colton, Clark K.; Powers, Daryl E.; Millman, Jeffrey R., Methods and compositions for enhanced differentiation from embryonic stem cells.
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Colton, Clark K.; Millman, Jeffrey R., Methods and compositions for increased safety of stem cell-derived populations.
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Colton, Clark K.; Millman, Jeffrey R., Methods and compositions for increased safety of stem cell-derived populations.
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Terzic, Andre; Behfar, Atta, Methods and materials for providing cardiac cells.
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Terzic, Andre; Behfar, Atta, Treating cardiovascular tissue.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Process for making transplantable cardiomyocytes from human embryonic stem cells 원문보기