IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
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출원번호 |
UP-0456843
(2006-07-11)
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등록번호 |
US-7732663
(2010-06-29)
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우선권정보 |
CA-2235978(1998-06-08); CA-2256121(1998-12-31) |
발명자
/ 주소 |
- Wang, Hong
- Fowke, Larry C.
- Crosby, William L.
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출원인 / 주소 |
- Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food
- University of Saskatchewan Technologies, Inc.
- The National Research Council of Canada
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대리인 / 주소 |
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인용정보 |
피인용 횟수 :
0 인용 특허 :
19 |
초록
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Methods for using cyclin-dependent kinase (CDK) inhibitor genes, or anti-sense constructs complementary to such genes, to modify the growth and development of plant cells and organs are disclosed. Also provided are methods of modifying the development of plant cells and plants by transforming plant
Methods for using cyclin-dependent kinase (CDK) inhibitor genes, or anti-sense constructs complementary to such genes, to modify the growth and development of plant cells and organs are disclosed. Also provided are methods of modifying the development of plant cells and plants by transforming plant cells with nucleic acids encoding cyclin-dependent kinase inhibitor polypeptides, or anti-sense constructs complementary to such nucleic acids, to produce transformed plant cells, and then culturing the plant cells or regenerating a plant under conditions wherein the cyclin-dependent kinase inhibitor, or the anti-sense construct, is expressed. A variety of CDK inhibitor genes, and corresponding anti-sense constructs, are disclosed for use in a variety of plants. The nucleic acid encoding the cyclin-dependent kinase inhibitor may be operably linked to a tissue-specific promoter. Other provided aspects are modified transgenic plants and plant tissues. Also provided are methods of identifying nucleic acids that encode cyclin-dependent kinase inhibitors that are active in plants to modify the development of the plant.
대표청구항
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What is claimed is: 1. A method of modifying development of a plant by downregulating expression of an ICK1 polypeptide, wherein the plant comprises an ICK1 nucleic acid sequence encoding the ICK1 polypeptide, comprising: transforming a plant cell with a nucleic acid sequence complementary to the I
What is claimed is: 1. A method of modifying development of a plant by downregulating expression of an ICK1 polypeptide, wherein the plant comprises an ICK1 nucleic acid sequence encoding the ICK1 polypeptide, comprising: transforming a plant cell with a nucleic acid sequence complementary to the ICK1 nucleic acid sequence; and growing the transformed plant cell or progeny of the transformed plant cell to produce a transformed plant under conditions wherein the nucleic acid sequence complementary to the ICK1 nucleic acid is transcribed in a proliferative tissue of the transformed plant to inhibit expression of the ICK1 polypeptide in the proliferative tissue of the transformed plant, thereby modifying development of the plant, wherein the ICK1 nucleic acid sequence comprises the nucleic acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3, or a nucleic acid sequence having at least 95% sequence identity with the nucleic acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3, and wherein the nucleic acid sequence complementary to the ICK1 nucleic acid sequence is at least 95% complementary to the full length ICK1 nucleic acid sequence. 2. The method of claim 1, wherein the ICK1 polypeptide comprises an amino acid sequence that is at least 95% identical to the sequence set forth in SEQ ID NO: 10. 3. The method of claim 1, wherein the plant is a member of the Cruciferae family. 4. The method of claim 1, wherein the plant is a member of the Brassica genus. 5. The method of claim 1, wherein the nucleic acid sequence complementary to the ICK1 nucleic acid sequence is complementary to a region of the ICK1 nucleic acid sequence encoding the amino acid sequence selected from the group consisting of positions 1-72, 1-108, 1-152, 1-191, 1-162, 3-72, 73-108, 73-191, 109-153, 130-191, 153-162, 163-175, 163-191, and 176-191 of the amino acid sequence set forth in SEQ ID NO: 2, or the corresponding nucleic acid positions as set forth in SEQ ID NO: 1, SEQ ID NO: 3, or the corresponding nucleic acid positions of the nucleic acid sequence having at least 95% sequence identity with the nucleic acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3. 6. The method of claim 1, wherein the nucleic acid sequence complementary to the ICK1 nucleic acid sequence is operably linked to a constitutive promoter. 7. The method of claim 1, wherein the nucleic acid sequence complementary to the ICK1 nucleic acid sequence is operably linked to a tissue-specific promoter. 8. The method of claim 7, wherein the tissue-specific promoter is the AP3 promoter. 9. The method of claim 7, wherein the tissue-specific promoter mediates expression of the nucleic acid sequence complementary to the ICK1 nucleic acid in petal or stamen primordia. 10. The method of claim 1, wherein modifying development of the plant makes the plant male sterile. 11. The method of claim 1, wherein modifying development of the plant modifies development of a tissue in the plant so that petals on the transformed plant are altered or absent. 12. A method for down-regulating expression of an ICK1 polypeptide in a plant cell, comprising: expressing in the plant cell an anti-sense oligonucleotide having a DNA sequence, or an equivalent RNA sequence, as set forth in SEQ ID NO: 1 or SEQ ID NO: 3, or a nucleic acid sequence at least 95% identical thereto. 13. A method of modifying development of a plant by down regulating expression of an ICK1 polypeptide, wherein the plant comprises an ICK1 nucleic acid sequence encoding the ICK1 polypeptide, comprising: transforming a plant cell with a nucleic acid sequence complementary to the ICK1 nucleic acid sequence; and growing the transformed plant cell or progeny of the transformed plant cell to produce a transformed plant under conditions wherein the nucleic acid sequence complementary to the ICK1 nucleic acid is transcribed in a proliferative tissue of the transformed plant to inhibit expression of the ICK1 polypeptide in the proliferative tissue of the transformed plant, thereby modifying development of the plant, wherein the ICK1 polypeptide comprises the sequence set forth in SEQ ID NO: 2, or a sequence having at least 95% sequence identity thereto, and wherein the nucleic acid sequence complementary to the ICK1 nucleic acid sequence is at least 95% complementary to the full length ICK1 nucleic acid sequence encoding the ICK1 polypeptide. 14. A method for down-regulating expression of an ICK1 polypeptide in a plant cell, comprising: expressing in the plant cell an anti-sense oligonucleotide that is at least 95% identical to a nucleic acid sequence encoding the ICK1 polypeptide of SEQ ID NO: 2.
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