Kits for diagnosis and monitoring of pathogenic infection by analysis of cell-free pathogenic nucleic acids in urine
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07H-021/04
A61K-009/14
C12N-009/12
출원번호
UP-0137934
(2005-05-25)
등록번호
US-7803929
(2010-10-21)
우선권정보
IT-RM2005A0068(2005-02-17)
발명자
/ 주소
Melkonyan, Hovsep
Cannas, Angela
Tomei, Louis David
Umansky, Samuil R.
출원인 / 주소
TrovaGene, Inc.
대리인 / 주소
Mintz Levin Cohn Ferris Glovsky and Popeo, P.C.
인용정보
피인용 횟수 :
13인용 특허 :
8
초록▼
The present invention relates to a method for diagnosing and/or monitoring a bacterial or parasitic infection by detection and quantification of the transrenal nucleic acids, derived from bacterial pathogenic agents or from parasites, in urine. The detection method optionally includes the isolation
The present invention relates to a method for diagnosing and/or monitoring a bacterial or parasitic infection by detection and quantification of the transrenal nucleic acids, derived from bacterial pathogenic agents or from parasites, in urine. The detection method optionally includes the isolation and the purification of the nucleic acids from urine by methods known in the art including pairing with molecular probes that are specific for the pathogenic agents, PCR hybridization, PCR, nested PCR, SSCP, LCR, and SDA. Diagnostic kits based on these detection methods are also claimed.
대표청구항▼
The invention claimed is: 1. A kit, comprising: a) one or more reagents or materials for the isolation and purification of cell-free pathogen-related transrenal nucleic acids from a urine sample of a subject, wherein said reagents or materials comprise a matrix including a silica-based resin, where
The invention claimed is: 1. A kit, comprising: a) one or more reagents or materials for the isolation and purification of cell-free pathogen-related transrenal nucleic acids from a urine sample of a subject, wherein said reagents or materials comprise a matrix including a silica-based resin, wherein said silica-based resin isolates cell-free nucleic acids that are less than about 300 nucleotides; and wherein said reagents or materials comprise an agent that inhibits the degradation of the cell-free nucleic acids; and b) two or more primers for detecting said cell-free pathogen-related nucleic acids in said urine sample, wherein said two or more primers are selected from the group consisting of SEQ ID NOs: 1-3. 2. The kit according to claim 1, further comprising reagents for the performance of a technique selected from the group consisting of a hybridization of said cell-free pathogen-related nucleic acids, a cycling probe reaction, a polymerase chain reaction, a nested polymerase chain reaction, a ligase chain reaction, and a strand displacement amplification. 3. The kit according to claim 1, further comprising a chaotropic agent. 4. The kit according to claim 1, wherein said agent that inhibits the degradation of the nucleic acids is selected from the group consisting of a ion-chelating agent, a denaturing agent, and an ionic detergent. 5. The kit according to claim 4, wherein said ion-chelating agent is EDTA; said denaturing agent is guanidine HCL or guanidine isothiocyanate; and said ionic detergent is N-lauryl sarcosine or sodium dodecyl sulfate. 6. A kit, comprising: a) one or more reagents or materials for the detection and quantitation of cell-free pathogen-related nucleic acids from a urine sample of a subject, wherein said reagents or materials comprise a matrix including a silica-based resin, wherein said silica-based resin isolates cell-free nucleic acids that are less than about 300 nucleotides; and wherein said reagents or materials comprise an agent that inhibits the degradation of the cell-free nucleic acids; and b) two or more primers for detecting and quantitating said cell-free pathogen-related nucleic acids in said urine sample, wherein said two or more primers are selected from the group consisting of SEQ ID NOs: 1-3. 7. The kit according to claim 6 further comprising reagents for the performance of a technique selected from the group consisting of a hybridization of said cell-free pathogen related nucleic acids, a cycling probe reaction, a polymerase chain reaction, a nested polymerase chain reaction, a ligase chain reaction, and a strand displacement amplification. 8. The kit according to claim 7, further comprising a chaotropic agent. 9. The kit according to claim 6, wherein said agent that inhibits the degradation of nucleic acids is selected from the group consisting of a ion-chelating agent, a denaturing agent, and an ionic detergent. 10. The kit according to claim 9, wherein said ion-chelating agent is EDTA; said denaturing agent is guanidine HCL or guanidine isothiocyanate; and said ionic detergent is N-lauryl sarcosine or sodium dodecyl sulfate.
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이 특허에 인용된 특허 (8)
McDonough Sherrol H. ; Ryder Thomas B. ; Yang Yeasing, Detection of human immunodeficiency virus type 1.
Sandhu Gurpreet S. ; Kline Bruce C. ; Stockman Leslie ; Roberts Glenn D. ; Lewis Marcia E., IS6110 based molecular detection of mycobacterium tuberculosis.
Leckie Gregor W. (Highland Park IL) Davis Alan H. (Vernon Hills IL) Semple-Facey Ingrid E. (Beach Park IL) Manlove Matthew T. (Vernon Hills IL) Solomon Natalie A. (Buffalo Grove IL), Materials and methods for the detection of Mycobacterium tuberculosis.
Crawford Jack T. (Atlanta GA) Eisenach Kathleen D. (Little Rock AR) Cave M. Donald (Little Rock AR) Bates Joseph H. (Little Rock AR), Repetitive DNA sequence specific for mycobacterium tuberculosis to be used for the diagnosis of tuberculosis.
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