[특허]Polymerase inhibitor and method of using same

[미국특허] Polymerase inhibitor and method of using same 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C07H-021/04 A61K-048/00
출원번호	UP-0551705 (2004-04-01)
등록번호	US-7820808 (2010-11-15)
국제출원번호	PCT/US2004/010029 (2004-04-01)
§371/§102 date	20060714 (20060714)
국제공개번호	WO04/090153 (2004-10-21)
발명자 / 주소	Moser, Michael James Marshall, David J. Prudent, James R. Van Hout, Christopher V. Larsen, Christine A.
출원인 / 주소	EraGen Biosciences, Inc.
대리인 / 주소	Foley & Lardner LLP
인용정보	피인용 횟수 : 0 인용 특허 : 41

초록 ▼

The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.

대표청구항 ▼

What is claimed is: 1. A polymerase inhibitor comprising a nucleic acid sequence a portion of which is double-stranded at or below the melting temperature of the nucleic acid sequence, wherein the double-stranded portion of the nucleic acid sequence is of sufficient length to be recognized by a polymerase as a template for extension except that the nucleic acid sequence is substantially incapable of being extended by the polymerase, wherein at least a 5′ nucleotide of the nucleic acid sequence is single stranded when the nucleic acid is at or below the melting temperature of the nucleic acid sequence. 2. The polymerase inhibitor of claim 1 wherein the polymerase inhibitor is not specific for a nucleic acid polymerase or family of polymerases related to the nucleic acid polymerase. 3. The polymerase inhibitor of claim 1 wherein the inhibitory activity of the polymerase inhibitor is not sequence specific. 4. The polymerase inhibitor of claim 1 wherein the nucleic acid sequence of the polymerase inhibitor does not act as a primer for an intended target nucleic acid. 5. The polymerase inhibitor of claim 1 wherein the nucleic acid sequence of the polymerase inhibitor does not form part of the nucleic acid that includes an intended target nucleic acid sequence. 6. The polymerase inhibitor claim 1 wherein the polymerase inhibitor consists essentially of a nucleic acid sequence. 7. The polymerase inhibitor of claim 1 wherein the 3′ terminal nucleic acid of the nucleic acid sequence comprises a blocking moiety that prevents extension of the 3′ terminal nucleic acid by the polymerase. 8. The polymerase inhibitor of claim 1 wherein the portion of the nucleic acid that is double-stranded at or below the melting temperature of the nucleic acid is formed by a single nucleic acid sequence that is capable of annealing to itself. 9. The polymerase inhibitor of claim 1 wherein the portion of the nucleic acid that is double-stranded at or below the melting temperature of the nucleic acid is formed by two separate nucleic acid sequences that at least partially anneal to one another. 10. The polymerase inhibitor of claim 1 wherein at least the 3′ terminal nucleic acid of the nucleic acid sequence is single stranded when the nucleic acid is at or below the melting temperature of the nucleic acid sequence. 11. The polymerase inhibitor of claim 1 wherein the nucleic acid portion of the polymerase inhibitor comprises DNA or a DNA mimetic. 12. The polymerase inhibitor of claim 1 wherein the nucleic acid comprises RNA or an RNA mimetic. 13. The polymerase inhibitor of claim 1 wherein the melting temperature of the double stranded portion of the nucleic acid portion of the polymerase inhibitor is in the range of about 25° C. to 80° C. 14. The polymerase inhibitor of claim 1 wherein the double stranded portion of the nucleic acid portion of the polymerase inhibitor is at least 10 bases in length. 15. A kit for inhibiting a nucleic acid polymerase comprising the polymerase inhibitor of claim 1. 16. The kit of claim 15 further comprising one or more additional reagents for carrying out a nucleic acid amplification reaction.

이 특허에 인용된 특허 (41) 인용/피인용 타임라인 분석

Martinez, Tomas Ramon, 3 blocked nucleic acid amplification primers.
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Jones Roger A. (Glenside PA) Fathip Reza (Newark NJ) Gaffney Barbara L. (Neshanic NJ), 6-O-substituted guanosine derivatives.
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Southern Edwin,GBX, Analying polynucleotide sequences.
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Rappaport Harry P. (Philadelphia PA), Artificial DNA base pair analogues.
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Steven Albert Benner, Chimeras of sulfur-linked oligonucleotide analogs and DNA and RNA.
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Kaiser Michael W. ; Lyamichev Victor I. ; Lyamichev Natasha, Cleavage of nucleic acid acid using thermostable methoanococcus jannaschii FEN-1 endonucleases.
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Nolan John P. ; White P. Scott ; Cai Hong, DNA polymorphism identity determination using flow cytometry.
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Wang Chao-huei Jeffrey ; Ammons Harryl ; Jolley Michael E., Detection of DNA/RNA by fluorescence polarization.
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Dale James Langham,AUX ; Timms Peter,AUX ; Walsh Terence Patrick,AUX, Detection of a nucleic acid sequence or a change therein.
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Brow Mary Ann D. ; Hall Jeff Steven Grotelueschen ; Lyamichev Victor ; Olive David Michael ; Prudent James Robert, Detection of nucleic acid sequences by invader-directed cleavage.
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Nadeau James G. ; Pitner J. Bruce ; Linn C. Preston ; Schram James L., Detection of nucleic acids by fluorescence quenching.
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Chandler Van S., Diode laser based measurement apparatus.
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Heller Michael J. (Poway CA) Jablonski Edward J. (San Diego CA), Fluorescent stokes shift probes for polynucleotide hybridization.
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Lee, Martin A; Fuerst, Roderick, Fluorimetric detection system of a nucleic acid.
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Gelfand David H. (Oakland CA) Holland Pamela M. (Oakland CA) Saiki Randall K. (Richmond CA) Watson Robert M. (Berkeley CA), Homogeneous assay system using the nuclease activity of a nucleic acid polymerase.
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Nikiforov Theo (Baltimore MD) Karn Jonathan (Little Shelord GBX) Goelet Philip (Cockeysville MD), Ligase/polymerase mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis.
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Fulton R. Jerrold, Method and compositions for flow cytometric determination of DNA sequences.
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Boyce-Jacino Michael T. ; Rogers Yu-Hui ; Goelet Philip, Method for analyzing the nucleotide sequence of a polynucleotide by oligonucleotide extension on an array.
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Kurn Nurith ; Liu Yen Ping ; Lishanski Alla ; Taylor Marc, Method for controlling the extension of an oligonucleotide.
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Benner Steven A. (Hadlaubstrasse 151 CH-8006 Zurich CHX), Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases.
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Benner Steven Albert, Method for selecting functional deoxyribonucleotide derivatives.
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Benner, Steven Albert, Method for selecting functional deoxyribonucleotide derivatives.
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Rust Stephan (Munster DEX) Funke Harald (Munster DEX) Assmann Gerd (Munster DEX) Kessler Christoph (Dorfen DEX) Rueger Rudiger (Seeshaupt DEX), Method of detecting variant nucleic acids.
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Fulton R. Jerrold, Methods and compositions for flow cytometric determination of DNA sequences.
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Sorge, Joseph A., Methods for detection of a target nucleic acid sequence.
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Benner, Steven Albert, Methods for preparing oligonucleotides containing non-standard nucleotides.
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Brenner Sydney (Cambridge GB2), Methods for sorting polynucleotides using oligonucleotide tags.
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Ravikumar Vasulinga T., Oligomeric phosphite, phosphodiester, Phosphorothioate and phosphorodithioate compounds and intermediates for preparing.
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Benner Steven Albert, Oligonucleotides with non-standard bases and methods for preparing same.
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Benner Steven Albert, Precursors for deoxyribonucleotides containing non-standard nucleosides.
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Benner Steven Albert,CHX, Predicting folded structures of proteins.
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Mullis Kary B. (Kensington CA), Process for amplifying nucleic acid sequences.
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Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences.
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Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
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Caruthers Marvin H. (Boulder CO) Matteucci Mark D. (Boulder CO), Process for preparing polynucleotides.
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Benner Steven Albert, Recognition of oligonucleotides containing non-standard base pairs.
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Collins Mark L. (Walnut Creek CA) Horn Thomas (Berkeley CA) Sheridan Patrick J. (San Leandro CA) Warner Brian D. (Martinez CA) Urdea Michael S. (Alamo CA), Reduction of nonspecific hybridization by using novel base-pairing schemes.
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Collins Mark L. ; Horn Thomas ; Sheridan Patrick J ; Warner Brian D. ; Urdea Michael S., Reduction of nonspecific hybridization by using novel base-pairing schemes.
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Grenier, Jennifer K.; Marshall, David J.; Prudent, James R.; Richmond, Craig S.; Roesch, Eric B.; Scherrer, Christopher W.; Sherrill, Christopher B.; Ptacin, Jerod L., Solid support assay systems and methods utilizing non-standard bases.
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Bodor Nicholas S. (Gainesville FL), Targeted drug delivery via phosphonate derivatives.
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Summerton James (Corvallis OR) Weller Dwight (Corvallis OR) Stirchak Eugene (Corvallis OR), Uncharged polynucleotide-binding polymers.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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[미국특허] Polymerase inhibitor and method of using same 원문보기