초록 ▼

A method of detecting a target nucleic acid is disclosed, the method comprising detecting the presence of a fluorescent covalent crosslinked product from non-fluorescent precursors. The fluorescent covalent crosslinked product comprises a novel fluorophore structure. Also described are methods of sy

A method of detecting a target nucleic acid is disclosed, the method comprising detecting the presence of a fluorescent covalent crosslinked product from non-fluorescent precursors. The fluorescent covalent crosslinked product comprises a novel fluorophore structure. Also described are methods of synthesizing probe molecules that can form fluorescent covalent crosslinked products with nucleic acid targets and arrays comprising such probes.

대표청구항 ▼

What is claimed is: 1. A method of determining the presence of a target nucleic acid molecule in a sample, said method comprising:contacting a probe molecule with a sample comprising a target nucleic acid molecule, wherein the probe molecule comprises one or more 5-halo-4-thiouracil nucleobases, whe

What is claimed is: 1. A method of determining the presence of a target nucleic acid molecule in a sample, said method comprising:contacting a probe molecule with a sample comprising a target nucleic acid molecule, wherein the probe molecule comprises one or more 5-halo-4-thiouracil nucleobases, wherein each of the one or more 5-halo-4-thiouracil nucleobases is a 5-chloro-4-thiouracil nucleobase or a 5-fluoro-4-thiouracil nucleobase, and wherein the target nucleic acid comprises a thymidine;irradiating the sample for a period of time at a wavelength at which the one or more 5-halo-4-thiouracil nucleobases is selectively photoexcited to form between a 5-halo-4-thiouracil nucleobase of the probe molecule and the thymidine of the target nucleic acid a fluorescent crosslinked product comprising a fluorophore having the structure of Formula (I):