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Capturing sequences adjacent to type-IIs restriction sites for genomic library mapping 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12Q-001/68
출원번호 US-0272680 (2008-11-17)
등록번호 US7985547 (2011-07-12)
발명자 / 주소
  • Sapolsky, Ronald J.
  • Lipshutz, Robert J.
  • Gingeras, Thomas R.
출원인 / 주소
  • Affymetrix, Inc.
대리인 / 주소
    Sandra E., Wells
인용정보 피인용 횟수 : 1  인용 특허 : 74

초록 ▼

The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs

대표청구항 ▼

What is claimed is: 1. A method for identifying sequences in a polynucleotide, comprising:(a) cleaving the polynucleotide with a first type-IIS endonuclease to obtain fragments;(b) ligating a first adapter sequence to the fragments generated in step (a) to obtain first adapter-ligated fragments, the

이 특허에 인용된 특허 (74)

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이 특허를 인용한 특허 (1)

  1. Du, Ye; Zhao, Meiru; Chen, Ying; Wu, Jinghua; Tian, Geng; Wang, Jun, DNA library, preparation method thereof, and device for detecting SNPs.
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