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| 국가/구분 | United States(US) Patent 등록 |
|---|---|
| 국제특허분류(IPC7판) |
|
| 출원번호 | US-0612445 (2006-12-18) |
| 등록번호 | US8030066 (2011-09-19) |
| 발명자 / 주소 |
|
| 출원인 / 주소 |
|
| 인용정보 | 피인용 횟수 : 2 인용 특허 : 402 |
The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In s
The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
What is claimed is: 1. A linear vector comprising:(a) an origin of replication,(b) a selection marker,(c) two att recombination sites which do not recombine with each other, wherein one of the att recombination sites has the seven base pair overlap sequence TTTGTAC and wherein the vector also contai
What is claimed is: 1. A linear vector comprising:(a) an origin of replication,(b) a selection marker,(c) two att recombination sites which do not recombine with each other, wherein one of the att recombination sites has the seven base pair overlap sequence TTTGTAC and wherein the vector also contains the sequence CCCTT at a terminus.
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